China Animal Husbandry & Veterinary Medicine ›› 2009, Vol. 36 ›› Issue (6): 30-33.

• 生理生化 • Previous Articles     Next Articles

Effect of Baicalin and Forsythiaside on the Excretion of IFN-α and IFN-γ in Pulmonary Microvascular Endothelial Cells

LIN Hong1，HU Ge1， YI Peng-fei2，JIANG Xiao-lin1，MU Xiang3   

  1. （1.Department of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China; 2.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 3.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing 102206, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20
  • Contact: MU Xiang

Abstract: To study the effect of baicalin and forsythiaside to induce rat pulmonary microvascular endothelial cells to express IFN-α and IFN-γ, the model was based on cultured PMVECs in vitro. With the method of ELISA, we detected the secretory volume of IFN-α and IFN-γ after PMVECs was induced by baicalin and forsythiaside at different concentration and time. The results indicated that PMVECs produced the most IFN-α and IFN-γ after induced 24 h by baicalin at 10 μg/mL, while forsythiaside was 20 μg/mL at 12 h and 5 μg/mL at 24 h， respectively. The result provided references for screening Chinese herbal medicinal constituents that could induce PMVECs to express IFN, and laid the foundation to further study the anti-virus mechanism of medicinal constituents.

Key words: baicalin; forsythiaside; pulmonary microvascular endothelial cells; IFN-α; IFN-γ

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