H7亚型禽流感病毒灭活疫苗的研制及其免疫效果研究

Abstract

Abstract: The H7N3 strain of avian influenza virus (AIV) was inoculated into chicken embryo, collected 72 h embryo fulid, after which it was inactivated and made into oil adjuvant vaccine.The antigen, physical characteristic, safety,efficacy, dosage were tested, according to the requirement of new biological product. Results showed the inactived vaccine was safe; its physical characterization was also fit for the requirement of the oil-adjuvant vaccine. 6 weeks later, the HI antibody rose to 29.4.The all vaccined poultry acquired protection challenged with AIV-H7N3 strain when HI antibody rose to 25, and the best immue dosage was 0.5 mL for each chick.The above results suggest that the vaccine can be used to prevent the epidemic of AIV-H7N3.

Key words: avian influenza; H7 subtype; inactivated vaccine; immune efficacy

CLC Number:

S858.3

SHI Hui;;LIAO Ming;LUO Kai-jian;REN Tao;ZHANG Gui-hong. Studies on Preparation and Immune Efficacy of H7 Subtype Avian Influenza Virus Inactivated Vaccine [J]. , 2009, 36(4): 154-156.

[1]	LIAO Siyu, ZHENG Xin, DAI Qi, XU Shiyi, ZHANG Tianxu, ZHANG Xiuqiao, GUI Chun. Study on the Anti-AIV-H9N2 Activity of the Ethyl Acetate Extract from Alternanthera philoxeroides in vitro and in vivo [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 759-769.
[2]	HE Ya, WANG Zhaohe, CHEN Bo, SONG Yuping, ZHOU Mingguang, XU Gaoyuan. Preparation of Inactivated Vaccine Against Porcine Pasteurella multocida Serotype A and Evaluation of Its Immune Effect [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3752-3761.
[3]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[4]	XIE Shouyu, WANG Ruimin, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, LONG Feng, QU Sujie, LU Wenjun, HUANG Jinshan, HUANG Fengmei, WEN Xinrui, YIN Yanwen, DENG Guohua. Molecular Characteristics of Complete Genomes of Two H3 Subtype Avian Influenza Virus Isolates Derived from Sea Duck in Guangxi Province [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1319-1328.
[5]	LIU Xin, PAN Jimai, HU Andong, ZENG Chengrong, YANG Ying, CHENG Zhentao, CHEN Jiangfeng, JIANG Haibo, WEN Ming. Effect of Astragalus Polysaccharides on Inactivated Aeromonas hydrophila Vaccine for Hybrid Sturgeon [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1177-1184.
[6]	LI Qi, TIAN Ye, HUANG Tao, LIU Cunxia. Research Progress of Nano-vaccine in the Prevention and Control of Avian Influenza [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 684-692.
[7]	JIN Huan, TU Min, SHI Aihua, ZHAO Lei, SHEN Jia, XI Shuo, ZHANG Jianwei, ZHANG Zhenhua. Isolation,Identification of H9 Subtype Avian Influenza Virus and Immune Protection Effect of Inactivated Trivalent Vaccine of Newcastle Disease,Avian Influenza (H9 Subtype),and Avian Adenovirus Disease Against the Viruses [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5013-5021.
[8]	WANG Yuchen, TIAN Guangyuan, ZHOU Yaping, GUO Ting, ZHAO Hongmei, SUN Yajie, ZHAO Fengmiao, BIAN Yuchen, YU Jialiang, HAO Yongqing. Prokaryotic Expression of BPIV3 NP Protein and Its Immunoenhancing Effect on the BPIV3 Inactivated Vaccine [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3122-3130.
[9]	MA Guangqiang, LIU Lijuan, CHEN Guoquan, WU Liangtao, YANG Jun, LIU Junze, LI Xiaomeng, PAN Chengwen, ZHOU Bijun. Preparation of Propolis Inactivated Vaccine of Riemerella anatipestifer Epidemic Strain in Guizhou [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3163-3170.
[10]	LI Qianqian, LONG Yunzhi, LIANG Gong, JIN Qing, LIU Jinjin, YANG Liu, HUANG Ying, YU Daobing, SONG Wenbo, HUANG Chao, TANG Xibiao. Screening of Vaccine Candidate Strains of Streptococcus suis Serotype 3 and Evaluation of the Immune Effect of the Bivalent Inactivated Vaccine Streptococcus suis Serotype 3+9 on Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2307-2317.
[11]	CHEN Siyu, SUI Zhuojun, WANG Zhanxin, LI Qiang, CHEN Yi, WANG Fei, CHEN Shuang, LIU Xinyi, YAN Zhuanqiang, HE Cheng. Immune Efficacy of Novel Nasal Vaccine of Inactivated EB Against Chlamydia psittaci Challenge [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1951-1959.
[12]	ZHAO Jing, CHU Ying, LUO Jun, GUO Xiaofeng. Construction and Immunogenicity of Recombinant Rabies Virus with Canine Distemper Virus H Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4776-4785.
[13]	YANG Jing, ZHANG Xinyu, LIANG Zhipeng, CHENG Qing, WANG Congying, CHI Shihong, YUAN Sheng, GUO Jinyue, HUANG Shujian, WEN Feng. Prokaryotic Expression of NP Protein of H9N2 Subtype Avian Influenza Virus and Preparation of Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3963-3971.
[14]	JIANG Ning, YIN Hang, ZHANG Yanwei, LI Zixin, CHI Xiaojuan, WANG Song. Research Progress on Co-infection of H9N2 Subtype Avian Influenza Virus with Other Pathogens [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3447-3455.
[15]	YIN Yanwen, SHI Kaichuang, SUN Wenchao, XIE Shouyu, QU Sujie, LU Wenjun, WANG Luxia, QIN Yong, PEI Xingbiao, LING Dan. Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 3002-3009.

Studies on Preparation and Immune Efficacy of H7 Subtype Avian Influenza Virus Inactivated Vaccine

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments