不同重悬细胞法在洗涤过程中对水牛外周血单个核细胞活率的影响

Abstract

Abstract: The peripheral blood mononuclear cell (PMBC) activity of buffalo was comported by different resuspension methods during washing process.The PBMC was separated by density gradient centrifugation，then cell was resuspended by transferpettor-spirting method ＆ centrifuge tube-sloshing method, when cell was washed three times the activity was evaluated. The cell activity by transferpettor-spirting method ＆ centrifuge tube-sloshing method was（74.9±2.6）% and（98.5±0.5）% respectively, the difference of cell activity was significant(P<0.01). The activity of PBMC was higher by centrifuge tube-sloshing, which could provide an useful method for further study that requiring higher cell activity. 

Key words: buffalo peripheral blood mononuclear cell; resuspension methods; cell activity

CLC Number:

S823.8⁺3

HAN Zhong-yan;ZHANG Bao-hong;HUANG Hai-yu;ZHANG Wei-yu;HUANG Wei-yi;ZHONG Heng-lu. Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process[J]. , 2009, 36(4): 100-102.

[1]	LIANG Shasha, YU Nongqi, LU Ying, LI Tingting, YAN Shengfei, HUANG Jian, TAN Zhengzhun, HUANG Rongchun, LI Hui, PAN Weijun, QIN Guangsheng. Screening of Key Genes Related to Intramuscular Fat Deposition in Buffaloes Based on GEO Database and Bioinformatics Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4359-4369.
[2]	XU Yuanyuan, ZHENG Haiying, YANG Chunyan, DENG Tingxian, HUANG Chenqian, FENG Chao, LU Xingrong, DUAN Anqin, MO Xia, MA Xiaoya, SHANG Jianghua. Effects of Inhibition of BMP/Smad Signaling Pathway on the Growth and Steroid Hormone Synthesis of Buffalo Ovarian Granulosa Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2354-2362.
[3]	XIAO Peng, SHANG Jianghua, YANG Chunyan, LI Mengqi, DUAN Anqin, MA Xiaoya, FENG Chao, HUANG Chenqian, ZHANG Bo, ZHOU Jinchen, WEI Kelong, ZHENG Wei, ZHENG Haiying. Effects of Alpha-linolenic Acid on Buffalo Ovarian Granulosa Cells Cultured in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2380-2387.
[4]	LIANG Shasha, PANG Chunying, DENG Tingxian, LU Xingrong, DUAN Anqin, MA Xiaoya, FANG Yanyan, LIANG Xianwei. Polymorphism of MFSD2A Gene and Its Association with Milk Production Traits in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3856-3865.
[5]	ZHENG Haiying, YANG Chunyan, LI Lingyu, TANG Liping, ZHENG Wei, SHANG Jianghua. Effects of Resveratrol on Proliferation of Cumulus Cells Cultured in vitro and mRNA Expression Levels of Related Genes in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 208-215.
[6]	TANG Na, JIANG Juncai, WANG Mengdi, CHA Xuedian, ZHANG Yongyun, YANG Zhong, LI Weizhen. Expression of Genes Related to Lipid Droplet Formation in Mammary Tissue of Dehong Dairy Buffalo with Different Milk Fat Contents [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4545-4553.
[7]	AIRIXIATI Dilixiati, ZHOU Zhengna, ZHANG Ye, LIU Zhiqiang, ZHANG Yafeng, ZHAO Xi, ABULIZI Wusiman. Effects of Crocin on the in vitro Maturation and Embryonic Development of Mouse Oocytes [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3734-3740.
[8]	DUAN Anqin, LU Xingrong, MA Xiaoya, LIANG Shasha, DENG Tingxian. Effect of LPIN1 Gene on Triglyceride and Fatty Acid Synthesis in Buffalo Mammary Epithelial Cell [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3140-3148.
[9]	ZHAO Xi, NURIBIYAMU·Maimaitituoheti, SONG Yukun, AIRIXIATI·Dilixiati, ZHANG Bo, AERMAN·Haire, ABULIZI·Wusiman. Effect of GDF9 on Sheep Cumulus Cell Proliferation [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3289-3296.
[10]	DENG Tongwei, XUE Yanan, LU Jianzhou, XIA Yanxun, CHEN Lulu, GUO Yiwen, PENG Liming, JIANG Zenghai, QIAO Hongxing, XU Yaohui, ZHANG Xiaozhan. Diagnosis and Pathogen Analysis of Mixed Infection of Porcine Reproductive and Respiratory Syndrome Virus,Porcine Circovirus Type 2 and Haemophilus parasuis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3401-3409.
[11]	LI Mengwei, PENG Lijuan, PENG Kaiping, XIE Fang, LIANG Xin, GUO Yangxia, YANG Chengjian. Effects of Inhibition of Different Rumen Microbial Activity on Rumen Fermentation and Fatty Acid Metabolism in Buffaloes [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2767-2778.
[12]	TANG Zhenhua, LI Kaikun, GUO Yanxia, LI Mengwei, XIE Fang, PENG Kaiping, LIANG Xin, YANG Chengjian. Research of Enrichmen Condition of Methanomassiliicoccales in Buffalo Rumen [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2464-2471.
[13]	JIAN Xinrui, QIAN Furong, WANG Jun, LI Xiaoxia, ZHANG Shaoxuan, ZHANG Zhibin, YU Yongsheng, GAO Kai, DIAO Yunfei, ZHANG Shumin, LI Zhaohua. Effect of Polyvinylpyrrolidone on Cryopreservation of Boar Semen [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2553-2560.
[14]	GUO Yanxia, LI Mengwei, PENG Lijuan, PENG Kaiping, TANG Zhenhua, LIANG Xin, XIE Fang, YANG Chengjian. Effects of Sodium Nitrate on Methane Production and Fatty Acid Hydrogenation Process of Buffalo in vitro Fermentation [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2071-2080.
[15]	HUA Liping, LI Zipeng, LI Jun, LIU Shuanghang, YANG Liguo, LIANG Aixin. Correlation Analysis Between Udder Morphology and Lactation Performance in Mediterranean Buffaloe [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2104-2112.

Influence of Buffalo PBMC Activity by Different Resuspension Methods During Washing Process

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments