水牛瘤胃Methanomassiliicoccales富集培养条件研究

doi:10.16431/j.cnki.1671-7236.2020.08.014

Abstract

Abstract: The aim of this study was to investigate the enrichmen condition of Methanomassiliicoccales from buffalo rumen,methanol,monomethylamine,dimethylamine or trimethylamine were used as substrates in BRN culture medium respectively.Methane production,population and diversity indices of Methanomassiliicoccales and methanogen,wasmeasure by gas chromatography,Real-time quantitative PCR and high-throughput sequencing of the 16S rRNA gene.The results showed that:The methane production of trimethylamine group was higher than other group significantly (P<0.05).The copy of Methanomassiliicoccales and methanogen of methanol group and trimethylamine group were higher than control group significantly(P<0.05) in 1st generation.The copy of Methanomassiliicoccales and methanogen of trimethylamine group were higher than control group,monomethylamine group,dimethylamine group significantly (P<0.05) in 7th generation.The copy of Methanomassiliicoccales was detected in trimethylamine group and dimethylamine group only in 23rd generation,and the copy of methanogen of trimethylamine group was highest.The result of Alpha diversity indices showed that,the Ace and Chao1 index of trimethylamine group were higher than control group significantly (P<0.05).The relative abundance of methanogen at order level were more than 90%.The methane production,population and relative abundance of Methanomassiliicoccales were increased while trimethylamine was used as substratesduring transfers.

Key words: buffalo; rumen; Methanomassiliicoccales; methane

CLC Number:

S823.8⁺3

TANG Zhenhua, LI Kaikun, GUO Yanxia, LI Mengwei, XIE Fang, PENG Kaiping, LIANG Xin, YANG Chengjian. Research of Enrichmen Condition of Methanomassiliicoccales in Buffalo Rumen[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2464-2471.

References

[1] CIAIS P,SABINE C,MANYOTHERS I,et al.IPCC AR5 WG1,chapter 6:Carbon and other biogeochemical cycles[R].2014.
[2] KUMAR S,INDUGU N,VECCHIARELLI B,et al.Associative patterns among anaerobic fungi,methanogenic archaea,and bacterial communities in response to changes in diet and age in the rumen of dairy cows[J].Frontiers in Microbiology,2015,6:781.
[3] JANSSEN P H,KIRS M.Structure of the archaeal community of the rumen[J].Applied and Environmental Microbiology,2008,74(12):3619-3625.
[4] DRIDI B,FARDEAU M L,OLLIVIER B,et al.Methanomassiliicoccus luminyensis gen.nov.,sp.nov.,a methanogenic archaeon isolated from human faeces[J].International Journal of Systematic and Evolutionary Microbiology,2012,62(8):1902-1907.
[5] POULSEN M,SCHWAB C,BORG JENSEN B,et al.Methylotrophic methanogenic thermoplasmata implicated in reduced methane emissions from bovine rumen[J].Nature Communications,2013,4:14-28.
[6] 成艳芬.厌氧真菌与产甲烷菌共培养系统的建立及其代谢与菌群变化的研究[D].南京:南京农业大学,2008. CHEN Y F.Establishment of consecutive batch cocultures of anaerobicfungi and methanogens from the rumen and study of themetabolism and microbial diversity in the co-cultures[D].Nanjing:Nanjing Agricultural University,2008.(in Chinese)
[7] HENDERSON G,COX F,GANESH S,et al.Rumen microbial community composition varies with diet and host,but a core microbiome is found across a wide geographical range[J].Scientific Reports,2015,5:145-167.
[8] LI Y,LEAHY S C,JEYAMALAR J.The complete genome sequence of the methanogenic archaeon iso4-h5 provides insights into the methylotrophic lifestyle of a ruminal representative of the Methanomassiliicoccales[J].Standards in Genomic Sciences,2016,11(1):59.
[9] DENMAN S E,TOMKINS N W,MCSWEENEY C S.Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane[J].FEMS Microbiol Ecology,2007,62(3):313-322.
[10] JIN W,CHENG Y,ZHU W.The community structure of methanomassiliicoccales in the rumen of Chinese goats and its response to a high-grain diet[J].Journal of Animal Science and Biotechnology,2017,8(4):906-915.
[11] LIU C,LI X H,CHEN Y X,et al.Age-related response of rumen microbiota to mineral salt and effects of their interactions on enteric methane emissions in cattle[J].Microbial Ecology,2016,73(3):590-601.
[12] LAN W,YANG C.Ruminal methane production:Associated microorganisms and the potential of applying hydrogen-utilizing bacteria for mitigation[J].Science of Total Environ,2019,654:1270-1283.
[13] MCALLISTER T A,MEALE S J,VALLE E,et al.Ruminant nutrition symposium:Use of genomics and transcriptomics to identify strategies to lower ruminal methanogenesis[J].Journal of Animal Science,2015,93(4):14-31.
[14] LANG K,SCHULDES J,KLINGL A,et al.New mode of energy metabolism in the seventh order of methanogens as revealed by comparative genome analysis of "Candidatus methanoplasma termitum"[J].Appl Environ Microbiology,2015,81(4):1338-1352.
[15] BORREL G,GACI N,PEYRET P,et al.Unique characteristics of the pyrrolysine system in the 7th order of methanogens:Implications for the evolution of a genetic code expansion cassette[J].Archaea,2014,21(37):41-46.
[16] MILLER T L.Methanosphaera stadtmaniae gen.nov.,sp.nov.:A species that forms methane by reducing methanol with hydrogen[J].Archives of Microbiology,1985,141:116-122.
[17] REA S,BOWMAN J P,POPOVSKI S,et al.Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov.,methanogens from the ovine and bovine rumen that can utilize formate for growth[J].International Journal of Systematic and Evolutionary Microbiology,2007,57(3):450-456.
[18] FRANZOLIN R,ST-PIERRE B,WRIGHT K N A A.Analysis of rumen methanogen diversity in water buffaloes (Bubalus bubalis) under three different diets[J].Microbial Ecology,2012,64(1):131-139.
[19] KHEJORNSART P,WANAPAT M.Diversity of rumen anaerobic fungi and methanogenic archaea in swamp buffalo influenced by various diets[J].Journal of Animal and Veterinary Advances,2010,9(24):3062-3069.
[20] MIN B R,SANDRA S,RAYMON S,et al.Gastrointestinal bacterial and methanogenic archaea diversity dynamics associated with condensed tannin-containing pine bark diet in goats using 16S rDNA amplicon pyrosequencing[J].International Journal of Microbio-logy,2014,18(23):141909.
[21] BORREL G,MCCANN A,DEANE J,et al.Genomics and metagenomics of trimethylamine-utilizing archaea in the human gut microbiome[J].The Isme Journal,2017,11(9):2059-2074.
[22] KELLY W J,LI D,LAMBIE S C,et al.Complete genome sequence of methanogenic archaeon iso4-g1,a member of the methanomassiliicoccales,isolated from a sheep rumen[J].Genome Announcements,2016,4(2):216-221.
[23] NARIHIRO T,KAMAGATA Y.Cultivating yet-to-be cultivated microbes:The challenge continues[J].Microbes and Environmerts,2013,28(2):163-165.
[24] BELANCHE A,DE LA FUENTE G,NEWBOLD C J.Study of methanogen communities associated with different rumen protozoal populations[J].FEMS Microbiol Ecology,2014,90(3):663-677.
[25] JIN W,CHENG Y F,MAO S Y,et al.Discovery of a novel rumen methanogen in the anaerobic fungal culture and its distribution in the rumen as revealed by Real-time PCR[J].BMC Microbiol,2014,14:104-114.
[26] ANDREA S,CLARISSA S,THOMAS W,et al.Phylogenetic and genomic analysis of Methanomassiliicoccales in wetlands and animal intestinal tracts reveals clade-specific habitat preferences[J].FEMS Microbiology Ecology,2015,92(1):fiv149.
[27] NOEL S J,HØJBERG O,URICH T,et al.Draft genome sequence of "candidatus methanomethylophilus" sp.1R26,enriched from bovine rumen,a methanogenic archaeon belonging to the methanomassiliicoccales order[J].Genome Announcements,2016,4(1):1715-1734.

Research of Enrichmen Condition of Methanomassiliicoccales in Buffalo Rumen

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