禽大肠杆菌的分离与16S rRNA的鉴定

Abstract

Abstract: Feces samples were collected from sick and dead chicken likely caused by Escherichina coli in a chicken farm，thepathogens were isolated and biochemically identificated, 6 bacterium were isolated from 8 feces samples. According to the highly conservative nature of bacterial 16S rRNA gene, a set of primers with broad range for Escherichina coli was designed，and 1 strain of bacteria was detected by polymerase chain reaction (PCR) and compared with 16S rRNA sequence of Escherichina coli from GenBank.The resembling rate can exceed 99%.The established PCR method is specific ,simple , easy, and available to identify Escherichina coli.

Key words: Escherichia coli; biochemical identification; 16S rRNA; PCR

CLC Number:

S852.61⁺2

SHI Fushan;ZHAO Deming. Isolation and Identification of 16S rRNA of Escherichia coli from Chicken〖[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(2): 111-113.

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Isolation and Identification of 16S rRNA of Escherichia coli from Chicken〖

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