禽大肠杆菌的分离与16S rRNA的鉴定

Abstract

Abstract: Feces samples were collected from sick and dead chicken likely caused by Escherichina coli in a chicken farm，thepathogens were isolated and biochemically identificated, 6 bacterium were isolated from 8 feces samples. According to the highly conservative nature of bacterial 16S rRNA gene, a set of primers with broad range for Escherichina coli was designed，and 1 strain of bacteria was detected by polymerase chain reaction (PCR) and compared with 16S rRNA sequence of Escherichina coli from GenBank.The resembling rate can exceed 99%.The established PCR method is specific ,simple , easy, and available to identify Escherichina coli.

Key words: Escherichia coli; biochemical identification; 16S rRNA; PCR

CLC Number:

S852.61⁺2

SHI Fushan;ZHAO Deming. Isolation and Identification of 16S rRNA of Escherichia coli from Chicken〖[J]. , 2009, 36(2): 111-113.

[1]	WANG Yongfei, DENG Bowen, LIU Xiaoyan, HAERLEHA·Amantai, GUO Jiadong, ZHOU Zhengguo, CAI Jiang, LI Youwen. Prokaryotic Expression of EF-Tu Protein of Mycoplasma ovipneumoniae and Preparation of Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 689-699.
[2]	CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.
[3]	GAO Ying, YU Yongtao, MA Yun, MA Zhengbing, YUAN Shuangjie, CUI Shengwei. Comparative Analysis of Diversity of Milk Microbiota in Cows with Mastitis and Healthy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5043-5055.
[4]	GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.
[5]	MA Baoyi, SHENG Chenyan, LIU Hongying, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, DU Rui, LENG Xue. Establishment of a Duplex TaqMan Real-time PCR Method for Detection of Bovine Viral Diarrhea Virus Types 1 and 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2326-2335.
[6]	LIANG Qian, BAO Taotao, XIAN Simei, YANG Qian, LI Pengfei, GU Qinglin, ZHENG Weihao, DU Peng, QING Chengxin. Establishment of PMA-PCR Method for Detection of Orf Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1524-1531.
[7]	JIANG Changjin, CHEN Yun, PAN Pengcheng, CHEN Baojian, GUAN Zhihui, LU Huilin, XIE Bingkun, QIN Zhaoxian. Fecal Microflora Structure and Its Correlation with Growth Rate in Finishing Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 924-931.
[8]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[9]	LYU Xiaonan, XU Lipu, ZHANG Wen, WANG Na, CAO Huan, WANG Xiaoliang, WANG Shu, WANG Jingbo. Research on Histopathological Changes and Virus Distribution Situation of Mirror Carps Infected with Carp Edema Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1077-1084.
[10]	QIAO Hongxing, DING Yong, ZHANG Liheng, SONG Yuzhen, DONG Qing, BIAN Chuanzhou, ZHAO Junqiang. Isolation, Identification, 16S rRNA Gene Sequencing and Genetic Evolution Analysis of Aeromonas aeruginosa from Duck [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 435-442.
[11]	ZHANG Yuying, LIU Shujie, FENG Yuzhe, ZHANG Xiaowei, CUI Zhanhong. Bacterial Flora Composition of Normal and Diarrhea Yak Calves Based on 16S rRNA Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3293-3302.
[12]	ZHANG Junfang, SUN Jianfu, SUN Bin, TANG Lin, WANG Ying, WANG Enze, CUI Yan, LI Qiang, YAN Changguo, LI Xiangzi. Cloning and Bioinformatics Analysis of PPARγ Gene of Yanbian Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2695-2704.
[13]	MIAO Manning, GUO Yiwen, HU Debao, ZENG Yuhan, LI Xin, ZHANG Linlin, DING Xiangbin, GUO Hong. Transcriptome Sequencing and Bioinformatics Analysis of MSTN Gene-edited Bovine Muscles [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2271-2281.
[14]	YANG Xiaobin, HE Lili, ZHANG Yang, ZHOU Ruifeng, HOU Kangli, ZHANG Yunyun, WANG Yueli, LI Qingqing, SHI Bingang, HU Jiang. Polymorphisms of LF Gene and Their Association with Milk Quality Traits in Yak [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1655-1663.
[15]	LI Heng, ZI Xiangdong. Cloning and Expression in Cumulus-oocyte Complexes of Yak TEDDM1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1284-1293.

Isolation and Identification of 16S rRNA of Escherichia coli from Chicken〖

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments