›› 2008, Vol. 1 ›› Issue (12): 89-92.

• 遗传繁育 • Previous Articles     Next Articles

Polymorphism Analysis of Exon 4 of EstrogenReceptor Gene in Sheep

DI Ran1, JIA Lihua2, CHU Mingxing1, CHEN Hongquan2, FANG Li1   

  1. 1. The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-20 Published:2008-12-20

Abstract: The estrogen receptor (ESR) gene was studied as a candidate gene for the high prolificacy of Small Tail Han sheep. A pair of primers was designed by sequence of exon 4 of ESR gene in human, chicken and rat. Single nucleotide polymorphisms (SNPs) of exon 4 of ESR gene were detected in both high prolificacy breeds (Small Tail Han and Hu sheep) and low prolificacy breeds (Texel, Chinese Merino, Corriedale and Dorper sheep) by polymerase chain reaction (PCR)single strand conformation polymorphism (SSCP). No polymorphism was detected in the amplified region in the six sheep breeds tested. The results indicated that the sequence of exon 4 of ESR gene was rather conserved, and exon 4 may not be the functional domain that affects the high prolificacy in sheep.

Key words: sheep; prolificacy; estrogen receptor gene; PCRSSCP