Abstract: The paper had reported the effect of COCs in vitro fertilization that were developed by three methods which one was sperm to be centrifuged in two different ways, and the second was sperm to be swimmingup in two different temperatures, and the third was COCs to be fertilized in three different sperm concentration. The experiment of sperm centrifuged had indicated that the sperm capacitation and oocyte in vitro fertilization rate of second upliquid were higher significantly than the second depositliquid’s.And it was a advancement method to centrifuge sperm in vitro fertilization at the low speed. The experiment of sperm swimmingup had indicated that the sperm capacitation and oocyte in vitro fertilization rate of constant temperature were higher significantly than the roomtemperature’s.The experiment of sperm concentration had indicated that the sperm capacitation and oocyte in vitro fertilization rate of middle sperm concentration（1.5×106/ml）were higher significantly than the high and low sperm concentration’s.

Key words: bovine; oocyte; sperm capacitation; in vitro fertilization; in vitro culture