蒙古绵羊Bcl-2基因3’端 cDNA的克隆及序列分析

Abstract

Abstract: In order to amplify the end of 3’of the Bcl2 gene of Mongolia sheep , we designed two pair of primers according to the reported Bos taurus Bcl-2 gene sequence in the GenBank .Total RNA was extracted from the spleen of Mongolia sheep and the cDNA encoding Mongolia sheep bcl-2 was amplified by the reverse transcription PCR(RT-PCR).The purified RT-PCR product was cloned in pBlueselect T vector.The results of restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing demonstrated that the Bcl-2 is belong to the family of Bcl-2 because the cDNA contain a open reading frame (ORF) of 261 bases which encoded 83 amino acid . The nucleotide sequences of Mongolia sheep Bcl-2 gene were compared with the counterpart sequences of Bos taurus , and the nucleotide homology was 95.8%.

Key words: sheep; Bcl-2 gene; cloning; sequence analysis; cDNA

CLC Number:

Q785

GUO Hongru;CAO Guifang;XI LIN Gaowa. he Cloning and Sequence Analysis of the end of 3’cDNA of Mongolia Sheep Bcl-2 Gene[J]. , 2008, 1(11): 28-31.

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he Cloning and Sequence Analysis of the end of 3’cDNA of Mongolia Sheep Bcl-2 Gene

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