Abstract: Experimental objective was to establish a model of oleic acid (OA)- acute lung injury (ALI) in pigs. The injection dosage and slaying methods were compared and the excellent one was selected. Then twelve and fifty-day pigs were randomly divided into two groups -control group and OA group. The breathing status of pigs were observed. The pigs were slaughtered in 1, 3 and 6 h, and the changes of structure and the quantity of containing water of lung were observed and compared. After injecting OA, the breathing manner of pigs changed from thorax-abdominal to pressing abdominal breath, and the frequency of breath became higher. And there were typical ALI pathological changes in the OA group when the pigs were examined in 1, 3 and 6 h after injection. It is successful to establish the model of acute lung injure in pigs via intravenous injection of 0.05 ml/kg avoirdupois OA by a few each time and more times. It contributes to the further study on porcine ALI and innate immune of the lung.

Key words: pigs; oleic acid; acute lung injury; animal model