Abstract: Matured ovine oocytes were activated by 5 μmol/L A23187 for 5 min an
d then 2 mmol/L 6-DMAP for 4 h,afterwards cultured in SOFaa and M-199 medium supplemented with BSA and serum of different concentrations, respectively. The cleavage rate of group M-199+FCS(29.9%) was significantly lower than SOFaa+BSA(52.7%) and SOFaa+FCS (52.8%) groups (P<0.05). And the blastocystrate of SOFaa+BSA(0.8%) group was significantily lower than M-199+BSA(7.6%),SOFaa+FCS(11.8%),M-199+FCS(10.2%) groups (P<0.05). The cleavage rate and blastocyst rate of oocyte treated with SOFaa+10% FCS（50.7%,11.3%）and SOFaa+20% FCS（59.2%,9.9%）were not significantly different, and those data of M-199+10% FCS（29.6%,5.6%） and M-199+20% FCS（32.4%,9.9%）were also same. These results showed that SOFaa medium is suitable for the development of ovine parthenogenetic embryos cultured in vitro , and SOFaa added with10% FCS can improve its function on those IVC embryos.

Key words: ovine; parthenogenesis; in vitro culture 