大肠杆菌肠菌素对空肠弯曲菌的促生长作用研究

doi:10.16431/j.cnki.1671-7236.2024.08.035

Abstract

Abstract: 【Objective】 The purpose of this experiment was to investigate the levels of enterobactin secreted by Escherichia coli isolated from the chicken intestines and the effects of enterobactin on Campylobacter jejuni growth promotion.【Method】 The indirect competitive ELISA (ic-ELISA) method was used to detect the production of enterobactin in 30 chicken enteric Escherichia coli isolates.The λRed homologous recombination system was used to construct the entB gene deletion mutant strain of Escherichia coli,and the enterobactin production of the parental and mutant strains was determined by ic-ELISA.The growth curves of the parental and mutant strains were determined in different iron-limited media.The supernatants from the parental and mutant strains were used to inoculate Campylobacter jejuni for investigating the effects of entericin secreted by Escherichia coli on the growth of Campylobacter jejuni.【Result】 The levels of enterobactin secreted by 30 chicken intestinal Escherichia coli isolates ranged from 11 to 126 μmol/L.2 entB gene deletion mutant strains of Escherichia coli were successfully constructed using λRed homologous recombination system. The qualitative results showed that Escherichia coli parental strains produced the yellow halo in the CAS medium,while no halo was observed for the entB gene deletion strain.ic-ELISA results showed that Escherichia coli parental strains produced enterobactin in the culture supernatants with a concentration of 100-150 μmol/L,while no enterobactin was detected for the entB gene deletion strain.In the iron-rich medium,there was no significant difference in the growth of Escherichia coli parental and entB gene deletion strains (P＞0.05).The D_{600 nm} values that reached the plateau in iron-limited medium were 0.6 and 0.3,respectively.The growth promotion results showed that the colony numbers of Campylobacter jejuni in the culture supernatant of Escherichia coli parental strain reached 7.8 and 8.2 lg CFU/mL at 24 and 36 h of inoculation,respectively.By comparison, the colony numbers in the culture supernatant of the entB gene deletion strain were 7.0 and 7.3 lg CFU/mL,respectively.【Conclusion】 Escherichia coli had the ability to secrete enterobactin for promoting the growth of Campylobacter jejuni in iron-limited environments.This study laid a foundation for further investigation of enterobactin-mediated interactions between intestinal bacteria.

Key words: Escherichia coli; enterobactin; entB gene; Campylobacter jejuni

CLC Number:

S831.7

SHI Chenxin, CUI Yifang, SHEN Xueyang, JIAO Xiaoli, GUO Fangfang, DING Baoan, XU Fuzhou. Effects of Escherichia coli Enterobactin on Growth Promotion of Campylobacter jejuni[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3585-3594.

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Effects of Escherichia coli Enterobactin on Growth Promotion of Campylobacter jejuni

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