番茄红素对顺铂所致大鼠脏器损伤的保护效果评价

doi:10.16431/j.cnki.1671-7236.2024.08.003

Abstract

Abstract: 【Objective】 The aim of this study was to evaluate the protective effect of lycopene (LCP) on organ injury induced by cisplatin (CDDP) in rats.【Method】 Thirty male Wistar rats were randomly divided into 5 groups (n＝6),which were control group (CON),CDDP and different LCP treatment groups (5 mg/kg,LLCP group;10 mg/kg,MLCP group;50 mg/kg,HLCP group).Rats in CON and CDDP groups were given 1 mL/kg corn oil per day,and rats in LLCP,MLCP and HLCP groups were given the corresponding concentration of LCP per day,and consecutive for 28 days.Except CON group,the other groups were given a single intraperitoneal injection of CDDP (7 mg/kg) on day 26.After the experiment,samples of stomach,small intestine (duodenum,jejunum and ileum),liver,kidney and blood were collected.The protective effects of different concentrations of LCP on CDDP-induced acute injury in rats were evaluated by physiological and biochemical indexes detection,HE staining,ELISA and Western blotting.【Result】 Compared with CON group,body weight,feed intake and water intake of rats in CDDP group were extremely significantly or significantly decreased (P＜0.01 or P＜0.05).Serum levels of aspartate aminotransferase (AST),urea nitrogen (BUN),creatinine (CRE) and malondialdehyde (MDA),interleukin-8 (IL-8),IL-6 and tumor necrosis factor-α (TNF-α) were significantly or extremely significantly increased (P＜0.05 or P＜0.01).The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were extremely significantly decreased (P＜0.01),and the histopathological injuries of stomach,small intestine,liver and kidney were serious.Compared with CDDP group,the body weight,feed intake and water intake of rats in MLCP group were significantly increased (P＜0.05),the levels of 6 biochemical indexes such as AST and ALT and the contents of MDA,IL-8,TNF-α and IL-6 in serum were significantly decreased (P＜0.05),and the organ histomathological damage was alleviated.Western blotting results showed that compared with CON group,the expression of TNF-α and IL-6 proteins in different tissues of rats in CDDP group were extremely significantly increased (P＜0.01),while the expression of IL-10 protein was extremely significantly decreased (P＜0.01).Compared with CDDP group,the expression of TNF-α and IL-6 proteins in all tissues of MLCP group were extremely significantly or significantly decreased (P＜0.01 or P＜0.05),the expression of IL-10 protein was extremely significantly or significantly increased (P＜0.01 or P＜0.05).【Conclusion】 LCP could improve the stomach,small intestine,liver and kidney injury in CDDP-induced rats by inhibiting oxidative stress and inflammation.Under this experiment conditions,10 mg/kg LCP had the best effect.

Key words: lycopene; cisplatin; rat; organ injury; inflammation

CLC Number:

S853

SUN Yue, TIAN Xue, YANG Chunxue, XU Enshuang, ZHENG Jiasan. Evaluation of the Protective Effect of Lycopene on Organ Injury Induced by Cisplatin in Rats[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3247-3255.

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Evaluation of the Protective Effect of Lycopene on Organ Injury Induced by Cisplatin in Rats

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