免疫磁性分离技术在病原微生物检测中的应用

doi:10.16431/j.cnki.1671-7236.2024.05.042

Abstract

Abstract: Immunomagnetic separation technology is a pre-treatment technology that separates the target adsorbed on the surface of the magnetic bead from the sample under the magnetic field.After the immune magnetic bead captures the target,the immunemagnetic bead-target complex is magnetically fixed on the tube wall,and the target can be obtained by elution of impurities.The enrichment effect of immunomagnetic separation technology is mainly affected by antibody specificity,magnetic bead diameter,magnetic bead addition amount,incubation time and sample pH,in which antibody specificity is the key to affect the enrichment effect of magnetic beads.Using the characteristics of immunomagnetic beads to adsorb antibodies,the coating step can be replaced in the enzyme-linked immunosorbent assay method,which can greatly reduce the detection time.As a pre-treatment method,immunomagnetic separation technology can improve the sensitivity and efficiency of the detection method when combined with other detection methods.In this review,the technical principle,general separation steps,common influencing factors and the application in enzyme-linked immunosorbent assay,immunochromatography,PCR,Real-time quantitative PCR,loop-mediated isothermal amplification reaction,flow cytometry,electrochemical sensors,liquid chromatography were described,so as to provide reference for the research and application of immunomagnetic separation technology.

Key words: immunomagnetic separation technology; sample preparation; pathogenic microorganism

CLC Number:

ZHANG Junhao, KUANG Lei, LEI Yifei, ZHAO Tianrui, XU Haojun, CHEN Bin, WANG Dan, WEI Yangfei, HONG Deng, HU Changmin. Application of Immunomagnetic Separation Technology in the Detection of Pathogenic Microorganisms[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 2219-2227.

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Application of Immunomagnetic Separation Technology in the Detection of Pathogenic Microorganisms

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