芪蓝复方对妊娠母猪免疫增强作用研究

doi:10.16431/j.cnki.1671-7236.2024.01.007

Abstract

Abstract: 【Objective】 This experiment was aimed to reveal the effect of Qilan compound on immune function of pregnant sows, provide reference for relieving immune suppression of pregnant sows, and provide data support for clinical application of Qilan compound.【Method】 The extract of Qilan compound was prepared by reflux heating and lyophilization, and its main active components were detected by phenol-sulfuric acid method, HPLC and UV spectrophotometry.25 Landrace binary sows (30-40 gestural age) were selected and divided 5 groups:Negative control group (group N), 0.1%, 0.2% and 0.4% Qilan compound groups (groups L, M and H) and positive control group (group A), 5 sows in each group.The experiment lasted for 30 days, and blood samples were collected on 1, 15 and 29 days.Serum biochemical indices (total protein, albumin, urea nitrogen, glucose, calcium, phosphorus and triglyceride), serum antioxidant function (total antioxidant capacity, glutathione peroxidase, superoxide dismutase and malondialdehyde), immunoglobulin (IgG, IgM and IgA), inflammatory factors (IL-6 and IL-1β) and PRRSV antibody levels were determined.【Result】 The contents of polysaccharide, epigoitrin and icariin in Qilan compound were 56.0%, 0.035% and 0.39%, respectively.On days 15 and 29 of gestation, compared with group N, the contents of serum albumin, IgM and IgA, total antioxidant capacity and glutathione peroxidase activity in group A were significantly increased (P<0.05).The activities of glutathione peroxidase and superoxide dismutase in groups L and H were significantly increased (P<0.05). The contents of malondialdehyde and IL-1β were significantly decreased in all groups (P<0.05). The S/P values in groups M and H were significantly decreased (P<0.05). Compared with group A, on day 15 of gestation, the levels of serum total protein, calcium, total antioxidant capacity and IgM and IgA contents in group L were significantly decreased (P<0.05). The activity of superoxide dismutase in group H was significantly increased, and the levels of serum total protein, calcium, triglyceride, IgM, IL-6 contents and S/P value were significantly decreased (P<0.05). On day 29 of gestation, serum total antioxidant capacity, serum calcium level and IgG, IgA contents in group L were significantly decreased (P<0.05). Serum calcium level, superoxide dismutase activity and IL-1β content in group M were significantly decreased (P<0.05).【Conclusion】 Qilan compound could significantly promote the immune function of pregnant sows, and it could be used for the prevention and control of immunosuppressive disease of pregnant sows.

Key words: Qilan compound; pregnant sows; immune function; immunosuppression

CLC Number:

S852.4

YANG Liyu, LI Yanling, FAN Shuyu, LI Jian. Study on Immunoenhancing Effect of Qilan Compound on Pregnant Sows[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 64-73.

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Study on Immunoenhancing Effect of Qilan Compound on Pregnant Sows

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