China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (11): 4424-4432.doi: 10.16431/j.cnki.1671-7236.2023.11.011

• Physiological and Biochemical • Previous Articles     Next Articles

Effect of LPS-induced Inflammatory Time on IAP and NF-κB Expression in Small Intestine of Mice

TAO Yanzi, WANG Qiuju, YUAN Ling, CUI Yizhe, JIA Junfeng   

  1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2023-03-08 Online:2023-11-05 Published:2023-10-27

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Abstract: 【Objective】 The purpose of this experiment was to investigate the changes of the expression levels of intestinal alkaline phosphatase (IAP) and nuclear transcription factor-κB (NF-κB) in mice with the prolonged inflammatory time induced by lipopolysaccharide (LPS), and provide a theoretical basis for reducing intestinal mucosal stress damage.【Method】 A total of 35 healthy male ICR mice with similar body weight and age were randomly divided into 7 groups with 5 mice in each group.The mice were intraperitoneally injected with 1 mL/mouse LPS (5 mg/kg).Before (0 h) and after injection (3, 6, 12, 24, 48 and 72 h) of LPS, the mice in each group were killed by neck dissection after anesthesia, and duodenum, jejunum and ileum tissues were collected.Real-time quantitative PCR and Western blotting were used to detect the mRNA and protein expression of IAP and NF-κB in each intestinal segment.The interaction between IAP and NF-κB was detected by co-immunoprecipitation.【Result】 Compared with 0 h, IAP gene expression in the small intestine of mice showed a wave change with the prolongation of LPS inflammatory time, the highest level was reached in duodenum and jejunum at 6 h (P < 0.01), and the highest level was reached in ileum at 48 h (P < 0.01).At 24 h, the expression of NF-κB gene in duodenum and jejunum were extremely significantly or significantly increased (P <0.01 or P < 0.05), there were no significant differences in expression levels at other time points (P > 0.05).After LPS treatment, the expression of IAP and NF-κB protein in each intestinal segment of mice showed a trend of first increasing and then decreasing, and the protein expression reached the highest level at 6 h (P < 0.05).The results of co-immunoprecipitation showed that NF-κB interacted with IAP.【Conclusion】 In the inflammatory process of LPS, the mRNA and protein expressions of NF-κB and IAP increased with the occurrence of intestinal inflammation, and reached the highest level at 6 h after inflammation, but gradually decreased with the extension of inflammatory time.There was a synergistic effect between IAP and NF-κB in the process of intestinal mucosal stress injury.

Key words: lipopolysaccharide; intestinal mucosal injury; IAP; NF-κB

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