新疆地区2株驴源马流产沙门菌的分离鉴定及致病性分析

doi:10.16431/j.cnki.1671-7236.2022.01.042

Abstract

Abstract: [Objective] The suspected pathogenic Salmonella causing abortion in two large-scale donkey farms in Xinjiang was determined, and its pathogenicity and drug resistance were explored. [Method] In this experiment, the isolated bacteria were identified by bacterial isolation and culture, morphological observation, physiological and biochemical test, and the flagella gene FliC of the isolated bacteria was amplified by PCR and sequenced. The pathogenicity of the isolated bacteria was identified and analyzed by pathogenicity determination, bacterial load detection and histopathological observation, and its drug resistance was analyzed by drug sensitivity test. [Result] Through bacterial isolation and culture, morphological observation and physiological and biochemical test, it was determined that the two strains of bacteria were Salmonella abortus equi and named G1-1 and XD1-2, respectively. The genetic evolution analysis of flagellum gene FliC of the two isolates showed that the similarity of FliC amino acid sequences between the two isolates was 99.0%, and the similarity between FliC amino acid sequences of the two isolates and Salmonella abortus equi isolates Ireland-HE801373 and Ireland-HE801378 was the highest, both of which were 99.3%. The isolates G1-1 was closely related to Ireland-HE801373, Ireland-HE801378, China-KJ486797.1 and China-KJ486769.1, and the isolates XD1-2 was closely related to USA-EBQ1214032.1. The similarity analysis were consistent with the phylogenetic tree results. The mouse pathogenicity test showed that the two isolates had strong toxicity to mice and different pathogenicity. The pathogenicity of XD1-2 to mice was stronger than that of G1-1. The isolated strain XD1-2 was resistant to 11 kinds of antibiotics and sensitive to 5 kinds of antibiotics. The isolated strain G1-1 was resistant to 9 kinds of antibiotics and sensitive to 11 kinds of antibiotics. [Conclusion] In this study, two strains of Salmonella abortus equi from different donkey farms were successfully isolated and identified, which had different drug sensitivity and virulence. In addition, the genetic evolution analysis result indicated that they were genetic different, and the evolutionary analysis of flagella gene FLIC also showed differences. The results provided a reference for the prevention and treatment of Salmonella abortus equi in donkey.

Key words: Salmonella abortus equi from donkey; isolation and identification; FliC gene; pathogenicity

CLC Number:

S855.1⁺2

PU Xiaofeng, ZHANG Yuwei, LI Shengnan, ZHANG Xuejing, SU Yan. Isolation, Identification and Pathogenicity Analysis of Two Salmonella abortus equi Strains from Donkeys in Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 395-403.

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Isolation, Identification and Pathogenicity Analysis of Two Salmonella abortus equi Strains from Donkeys in Xinjiang

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