唾液乳酸杆菌TCSL1对幼犬生长性能、血液学与血清生化指标及肠道健康的影响

doi:10.16431/j.cnki.1671-7236.2020.11.040

Abstract

Abstract: The aim of this study was to evaluate the effects of Lactobacillus salivarius TCSL1 on the growth performance,hematology and serum biochemical indexes and intestinal health of puppies,which was to lay a foundation for the research and development of probiotics for puppies.24 healthy six-week-old Chinese garden dogs were randomly divided into 4 groups,6 dogs were in each group.The experimental groups were fed with low (5×10⁶ CFU/mL),medium (5×10⁸ CFU/mL) and high dose (5×10¹⁰ CFU/mL) Lactobacillus salivarius TCSL1 orally for 28 days,respectively.At the same time,the control group took saline orally for 28 days.During the experiment,the behavior,diet and diarrhea were observed and recorded regularly.After 28 d,dogs were weighed respectively to calculate the gained weight.Blood samples were collected to detect hematology,serum biochemistry,intestinal barrier and inflammatory cytokines.Feces were collected to detect the content of secretory immunoglobulin sIgA and the number of main intestinal bacteria.The results showed that compared with the control group,all the experimental groups could promote the weight growth of puppies,reduced the diarrhea rate,and had a positive correlation with the oral dose.The number of lymphocyte,erythrocyte,immunoglobulin and alkaline phosphatase content increased,while alanine transaminase and creatinine decreased,but there were significant differences between the groups (P<0.05).With the increase of oral Lactobacillus salivarius dose,the content of intestinal barrier indicator factors such as diamine oxidase (DAO),D-lactate and lipopolysaccharide (LPS) in the serum of the experimental groups showed a significant decrease (P<0.05).The contents of TNF-α and IL-6 in serum of the experimental groups decreased significantly (P<0.05),while the contents of TGF-β1 and IL-10 increased significantly (P<0.05).The content of secretory of immunoglobulin sIgA in the feces of the experimental groups increased significantly (P<0.05),the number of coliform and Enterococcus in the feces of the experimental groups decreased significantly (P<0.05),the number of Lactobacillus and Bifidobacterium increased significantly (P<0.05).The above results showed that the oral Lactobacillus salivarius TCSL1 could improve the growth performance of puppies,enhanced the intestinal health and reduced the frequency of diarrhea.5×10¹⁰ CFU/mL per day of the oral Lactobacillus salivarius TCSL1 had the best effect.

Key words: puppies; Lactobacillus slivarius TCSL1; growth performance; intestinal health; probiotic

CLC Number:

S852.61

TIAN Qizhen, TANG Chenchen, SHEN Danqing, LU Wei, CAI Bingyan. The Effects of Lactobacillus salivarius TCSL1 on Growth Performance,Hematology, Serum Biochemical Indexes and Intestinal Health in Puppies[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3757-3766.

References

[1] CHAUCHEYRAS-DURAND F,DURAND H.Probiotics in animal nutrition and health[J].Beneficial Microbes,2010,1(1):3-9.
[2] ZAWISTOWSKA-ROJEK A,TYSKI S.Are probiotic really safe for humans?[J].Polish Journal of Microbiology,2018,67(3):251-258.
[3] STROMPFOVA V,MARCINAKOVA M,SIMONOVA M.Application of potential probiotic Lactobacillus fermentum AD1 strain in healthy dogs[J].Anaerobe,2006,12(2):75-79.
[4] BIAGI G,CIPOLLINI I,POMPEI A,et al.Effect of a Lactobacillus animalis strain on composition and metabolism of the intestinal microflora in adult dogs[J].Veterinary Microbiology,2007,124(1-2):160-165.
[5] 殷亚杰,聂春雨,翟健程,等.圈养驯鹿粪便中免疫球蛋白含量测定与相关性分析[J].黑龙江畜牧兽医,2019,11:158-161. YIN Y J,NIE C Y,ZAI J C,et al.Determination and correlation analysis of immunoglobulin content in feces of reindeer in captivity[J].Heilongjiang Animal Science and Veterinary Medicine,2019,11:158-161.(in Chinese)
[6] 赵东方.罗伊氏菌乳酸杆菌的分离鉴定及其抵抗仔猪感染F4⁺ ETEC效果的分析[D].哈尔滨:东北农业大学,2019. ZHANG D F.Isolation and identification of Lactobacillus reuteri and its effection on F4⁺ ETEC infection piglets[D].Harbin:Northeast Agricultural University,2019.(in Chinese)
[7] HEENEY D D,GAREAU M G,MARCO M L.Intestinal Lactobacillus in health and disease,a driver or just along for the ride?[J].Current Opinion in Biotechnology,2018,49:140-147.
[8] BAE J Y,KIM J I,PARK S,et al.Effects of Lactobacillus plantarum and Leuconostoc mesenteroides probiotics on human seasonal and avian influenza viruses[J].Journal Microbiol Biotechnol,2018,28(6):893-901.
[9] TIMMERMAN H M,VELDMAN A,ELSENE E V D,et al.Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics[J].Poultry Science,2006,85(8):1383-1388.
[10] MCCOY S,GILLILAND S E.Isolation and characterization of Lactobacillus species having potential for use as probiotic cultures for dogs[J].Journal of Food Science,2007,72(3):M94-M97.
[11] 孙宁.乳酸菌对仔犬生长性能、发病率、粪中大肠杆菌数量的影响[J].经济动物学报,2012,16(1):16-21. SUN N.Effects of L.acidophilus on the growth,morbidity and the number of E.coli of puppy[J].Journal of Economic Animal,2012,16(1):16-21.(in Chinese)
[12] 陆江,朱道仙,卢鹏飞,等.补喂复合益生菌制剂对幼犬生长性能、肠道动力及肠道屏障功能的影响[J].动物营养学报,2019,31(9):4242-4250. LU J,ZHU D X,LU P F,et al.Effects of supplementary feeding complex-probiotic-preparation on growth performance,intestinal motility and intestinal barrier function of puppies[J].Chinese Journal of Animal Nutrition,2019,31(9):4242-4250.(in Chinese)
[13] BAILLON M L,MARSHALL-JONES Z V,BUTTERWICK R F.Effects of probiotic Lactobacillus acidophilus strain DSM13241 in healthy adult dogs[J].American Journal of Veterinary Research,2004,65(3):338-343.
[14] STROMPFOVÁ V,KUBASOVA I,LAUKOVA A.Health benefits observed after probiotic Lactobacillus fermentum CCM 7421 application in dogs[J].Applied Microbiology and Biotechnology,2017,101(16):6309-6319.
[15] 胡红莲,高民.肠道屏障功能及其评价指标的研究进展[J].中国畜牧杂志,2012,48(17):78-82. HU H L,GAO M.Research advance in intestinal barrier function and evaluation index[J].Chinese Journal of Animal Science,2012,48(17):78-82.(in Chinese)
[16] WANG H,GONG J,WAGN W,et al.Are there any different effects of Bifidobacterium,Lactobacillus and Streptococcus on intestinal sensation,barrier function and intestinal immunity in PI-IBS mouse model?[J].PLoS One,2014,9(3):e90153.
[17] KATSANOS K H,PAPADAKIS K A.Inflammatory bowel disease:Updates on molecular targets for biologics[J].Gut and Liver,2017,11(4):455-463.
[18] WANG B,CHEN J,WANG S,et al.Lactobacillus plantarum L9 but not Lactobacillus acidophilus LA reduces tumour necrosis factor induced bacterial translocation in Caco-2 cells[J].Beneficial Microbes,2017,8(3):497-505.
[19] WANG J,JI H,WANG S,et al.Probiotic Lactobacillus plantarum promotes intestinal barrier function by strengthening the epithelium and modulating gut microbiota[J].Frontiers in Microbiology,2018,9:1953.
[20] KOZAKOVA H,SCHWARZER M,TUCKOVA L,et al.Colonization of germ-free mice with a mixture of three Lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization[J].Cellular & Molecular Immunology,2016,13(2):251-262.
[21] SICHETTI M,DEMARCO S,PAGIOTTI R,et al.Anti-inflammatory effect of multistrain probiotic formulation (L. rhamnosus,B. lactis and B. longum)[J].Nutrition,2018,53:95-102.
[22] MARCINAKOVA M,SIMONOVA M,STROMPFOVA V,et al.Oral application of Enterococcus faecium strain EE3 in healthy dogs[J].Folia Microbiologica (Praha),2006,51(3):239-242.
[23] KELLEY R L,PARK J S,OMAHONY L,et al.Safety and tolerance of dietary supplementation with a canine-derived probiotic (Bifidobacterium animalis strain AHC7) fed to growing dogs[J].Veterinary Therapeutics,2010,11(3):E1-E14.
[24] NAKAMURA A,OHNISHI Y,SHIROTORI K,et al.Evaluation of viability Bifidobacterium animalis subsp.lactis LKM512 in dogs[J].Beneficial Microbes,2015,6(6):791-797.
[25] KUMAR S,PATTANAIK A K,SHARMA S,et al.Probiotic potential of a Lactobacillus bacterium of canine faecal-origin and its impact on select gut health indices and immune response of dogs[J].Probiotics and Antimicrobial Proteins,2017,9(3):262-277.
[26] XU H,HUANG W,HOU Q,et al.Oral administration of compound probiotics improved canine feed intake,weight gain,immunity and intestinal microbiota[J].Frontiers in Immunology,2019,4(10):666.
[27] ZHANG J H,DENG J,WANG Z S,et al.Modulatory effects of Lactobacillus salivarius on intestinal mucosal immunity of piglets[J].Current Microbiology,2011,62(5):1623-1631.

The Effects of Lactobacillus salivarius TCSL1 on Growth Performance,Hematology, Serum Biochemical Indexes and Intestinal Health in Puppies

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