CsA对LPS诱导的子宫内膜基质细胞中IL-1β和IL-6表达的影响

doi:10.16431/j.cnki.1671-7236.2020.10.035

Abstract

Abstract: The aim of this study was to investigate the effect of immunosuppressant cyclosporine A (CsA) on the expression of proinflammatory cytokines interleukin 1 beta (IL-1β) and interleukin 6 (IL-6) in LPS-induced uterine endometrial stromas cells (ESCs).After cultured and identified in vitro,the inflammation model of the ESCs of female rabbits were successfully constructed by 1 μg/mL lipopolysaccharide (LPS),and 0 μg/mL LPS was used as the control.Then the expression levels of IL-1β and IL-6 were detected after treatment with 0,50,100,200 and 500 ng/mL CsA.The results showed that LPS significantly induced the expression of IL-1β and IL-6 in ESCs (P<0.05).Although CsA had no effect on the expression of IL-1β and IL-6,but 50,100,200 and 500 ng/mL CsA extremely significantly inhibited the upregulation of IL-1β and IL-6 induced by 1 μg/mL LPS (P<0.01).It suggested that a certain dose of CsA could effectively inhibit the LPS-induced immune stress in ESCs.This study provided a basis for the feasibility of CsA treatment after the genital tract of female infected by gram-negative bacteria,but its clinical application and mechanism still need to be further studied.

Key words: cyclosporine A; lipopolysaccharide; interleukin 1 beta; interleukin 6; immune stress

CLC Number:

S829.1

CHEN Mutian, REN Yan, ZHANG Lei, LI Jing, XIE Sijie, ZHANG Yan, ZHANG Ming, MEI Xiuli. Effect of CsA on the Expression of IL-1β and IL-6 in Endometrial Stomas Cells Induced by LPS[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3351-3360.

References 52

[1]	茹改珍,黄元华,孙莹璞.环孢素A及其在产科和生殖领域应用的研究[J].国际生殖健康/计划生育杂志,2011,30(3):230-233. RU G Z,HUANG Y H,SUN Y P.Researches in CsA and it's application in obstetrics and reproduction[J].Journal of International Reproductive Health/Family Planning,2011,30(3):230-233.(in Chinese)
[2]	贾暖,董玉波.FPIA与CMIA测定环孢素A血药浓度的相关性[J].实用医药杂志,2015,32(11):1030-1032. JIA N,DONG Y B.The correlation between FPIA and CMIA in the determination of cyclosporine A in blood[J].Practical Journal of Medicine & Pharmacy,2015,32(11):1030-1032.(in Chinese)
[3]	杨燕宁,朱伽月,宋秀胜,等.环孢素A临床应用的研究进展[J].国际眼科杂志,2017,17(3):463-466. YANG Y N,ZHU J Y,SONG X S,et al.Current progress on clinical application of cyclosporine A[J].International Eye Science,2017,17(3):463-466.(in Chinese)
[4]	茹改珍.环孢素A对小鼠早期胚胎体外发育的影响[D].郑州:郑州大学,2010. RU G Z.The effect of cyclosporine A on the development of mouse early embryos in vitro[D] Zhengzhou:Zhengzhou University,2010.(in Chinese)
[5]	王雁玲.MDS患者骨髓MSCs分子遗传学分析和CsA对MDS患者骨髓MSCs增殖凋亡影响的研究[D].太原:山西医科大学,2011. WANG Y L.Molecular genetical analysis of the bone marrow mesenchynal stromal cells in myelodysplastic syndrome patients and the study on cyclosporine's effects on their proliferation and apoptiosis[D].Taiyuan:Shanxi Medical University,2011.(in Chinese)
[6]	MAOR W,HILMI A,YAEL R,et al.The use of cyclosporine in respiratory diseases[J].Current Respiratory Medicine Reviews,2013,9(5):344-348.
[7]	NULMAN I,SGRO M,BARRERA M,et al.Long-term neuro development of children exposed in utero to ciclosporin after maternal renal transplant[J].Pediatric Drugs,2010,12(2):113-122.
[8]	FU J H,LI L,QI L J,et al.A randomized controlled trial of etanercept in the treatment of refractory recurrent spontaneous abortion with innate immune disorders[J].Taiwanese Journal of Obstetrics & Gynecology,2019,58(5):441-445.
[9]	CHEN C,HE Z B,ZHANG M,et al.Expression pattern of Toll-like receptors (TLRs) in different organs and effects of lipopolysaccharide on the expression of TLR2 and TLR4 in reproductive organs of female rabbit[J].Developmental and Comparative Immunology,2014,46(5):431-348.
[10]	兰瑞霞.LPS对母兔下丘脑、卵巢和输卵管上生殖激素受体表达影响的研究[D] 成都:四川农业大学,2014. LAN R X.Studies on the effect of lipopolysaccharide on the expression of reproductive hormone receptors in hypothalamus,ovary and oviduct of female rabbits[D].Chengdu:Sichuan Agricultural Univer-sity,2014.(in Chinese)
[11]	GREENK A,ZAREKS M,CATHERINOW H.Gynecologic health and disease in relation to the microbiome of the female reproductive tract[J].Fertility and Sterility,2015,104(6):1351-1357.
[12]	李凤雪,周翼飞,宫建美,等.脂多糖对小鼠子宫内膜Toll样受体4介导的炎性信号通路的影响[J].畜牧与兽医,2013,45(4):22-26. LI F X,ZHOU Y F,GONG J M,et al.Effect of LPS on inflammatory signal pathway mediated by TLR4 in endometrium of mice[J].Animal Husbandry & Veterinary Medicine,2013,45(4):22-26.(in Chinese)
[13]	苏晓健,赵永旺,管远红.脂多糖对小鼠子宫内膜中TLR4和TNF-α的影响[J].黑龙江畜牧兽医,2015,24:152-153. SU X J,ZHAO Y W,GUAN Y H.Effects of lipopolysaccharide on TLR4 and TNF-α in mouse endometrium[J].Heilongjiang Animal Science and Veterinary Medicine,2015,24:152-153.(in Chinese)
[14]	殷凡,宁铂涛.脂多糖在脓毒症中的研究进展[J].中国小儿急救医学,2019,26(7):533-536. YIN F,NING B T.Advanced research of lipopolysaccharide in sepsis[J].Chinese Pediatric Emergency Medicine,2019,26(7):533-536.(in Chinese)
[15]	钱国军.AOAH通过降解肠道LPS促进过敏性哮喘[A].中国免疫学会.第十三届全国免疫学学术大会分会场交流报告[C].2018. QIAN G J.AOAH promotes allergic asthma by degrading intestinal LPS[A].Chinese Society of Immunology.Exchange Report of the 13th National Immunology Academic Conference[C].2018.(in Chinese)
[16]	邢文明.分泌蛋白CRISPLD2在脂多糖固有免疫应答中的功能研究[D].上海:华东理工大学,2008. XING W M.Study on the function of secreted protein CRISPLD2 in innate immune response of lipopoly-saccharide[D].Shanghai:East China University of Science and Technology,2008.(in Chinese)
[17]	何自标.LPS对母兔下丘脑、卵巢和输卵管中TLR4、TLR2及其相关免疫细胞因子表达的影响[D].成都:四川农业大学,2014. HE Z B.Effects of lipopolysaccharide on the expression of toll-like receptor 2,4 and related immune cytokines in hypothalamus,ovary and oviduct of female rabbit[D].Chengdu:Sichuan Agricultural University,2014.(in Chinese)
[18]	黄俊,吴长有.环孢素A对LPS诱导的早期炎症反应的影响[J].免疫学杂志,2010,26(12):1022-1024. HUANG J,WU C Y.Effects of cyclosporin A on early inflammatory reaction induced by LPS[J].Immuno-logical Journal,2010,26(12):1022-1024.(in Chinese)
[19]	LOSA J E,MATEOS R F,JIMENEZ L A.Effect of cyclosporin A on inflammatory cytokine production by human alveolar macrophages[J].Respiratory Medicine,1998,92:722-728.
[20]	HARTEL C,FRICKE L,SCHUMACHER N,et al.Delayed cytokine mRNA expression kinetics after T-lymphocyte costimulation:A quantitative measure of the efficacy of cyclosporin A-based immunosuppre-ssion[J].Clinical Chemistry,2002,48(12):2225-2231.
[21]	胡先勤,付小哲,董星星,等.鳜亲环蛋白A在传染性脾肾坏死病毒增殖中的作用[J].水产学报,2016,40(1):100-109. HU X Q,FU X Z,DONG X X,et al.Effect of mandarin fish cyclophilin A on the multiplication of infectious spleen and kidney necrosis virus[J].Journal of Fisheries of China,2016,40(1):100-109.(in Chinese)
[22]	杨丽琴.CsA对LPS诱导的母兔生殖道中免疫细胞因子表达的影响[D].雅安:四川农业大学,2019. YANG L Q.Effects of cyclosporin A on lipopolysaccharide-induced immune cytokines production in genital tract of female rabbit[D].Ya'an:Sichuan Agricultural University,2015.(in Chinese)
[23]	李瑞梅.家兔子宫内膜及平滑肌细胞体外分离及扩增的研究[D].杨凌:西北农林科技大学,2007. LI R M.Isolation and amplification in vitro of rabbit endometrium and smooth muscle cells[D].Yangling:Northwest A&F University of Science and Technology,2007.(in Chinese)
[24]	董蓉蓉.人子宫内膜干/祖细胞的分离、培养与人子宫内膜细胞异种移植效果观察[D].福州:福建医科大学,2014. DONG R R.Isolation,cultivation of human endometrial stem/progenitor cells and observation of xenotransplantation human endometrial cells[D].Fuzhou:Fujian Medical University,2014.(in Chinese)
[25]	赵诚悦,马红,郭镇华,等.猪子宫内膜基质细胞和腺上皮细胞的分离培养[J].吉林农业大学学报,2013,35(4):471-474. ZHAO C Y,MA H,GUO Z H,et al.Isolation and culture of stromal cells and gland epithelial cells of endometrium in pig[J].Journal of Jilin Agricultural University,2013,35(4):471-474.(in Chinese)
[26]	陈继明,高红艳,李沁,等.小鼠子宫内膜基质细胞的分离纯化与体外培养[J].中国计划生育和妇产科,2015,7(1):37-40. CHEN J M,GAO H Y,LI Q,et al.Isolation,purification and culture in vitro of stromal cells of mouse endometrium[J].Chinese Journal of Family Planning & Gynecotokology,2015,7(1):37-40.(in Chinese)
[27]	张明,郑洁,甘潇,等.孕酮、干扰素τ对牛子宫内膜上细胞外基质及其相关配体表达的影响[J].畜牧兽医学报,2013,44(2):309-315. ZHANG M,ZHENG J,GAN X,et al.Effects of progesterone and interferon τ on the expression of extracellular matrix and related ligands in bovine endometrium[J].Chinese Journal of Animal and Veterinary Sciences,2013,44(2):309-315.(in Chinese)
[28]	徐以民,何俊琳,刘学庆,等.一种从子宫内膜高质量提取总RNA的方法[J].中国优生与遗传杂志,2006,14(5):39-40. XU Y M,HE J L,LIU X Q,et al.An improved method of extracting high guality total RNA from endometrium in mice[J].Chinese Journal of Birth Health and Heredity,2006,14(5):39-40.(in Chinese)
[29]	何金鑫,黄丽华.LPS-TLR4/NF-κB信号通路与抗炎免疫研究进展[J].中外健康文摘,2013,(37):27-29. HE J X,HUANG L H.Research progress of LPS-TLR4/NF-κB signaling pathway and anti-inflamma-tory immunity[J].World Health Digest,2013,(37):27-29.(in Chinese)
[30]	杨一新,杨云波,周鸣,等.鼻咽癌细胞5-8F通过TLR4介导的信号通路对革兰氏阴性细菌脂多糖的反应性研究[J].生物化学与生物物理进展,2007,34(2):138-145. YANG Y X,YANG Y B,ZHOU M,et al.The study of nasopharygeal carcinoma cell 5-8F responsiveness to negative gram's lipopolysaccharide via TLR4-regulating signaling pathway[J].Progress in Bioche-mistry and Biophysics,2007,34(2):138-145.
[31]	FENG G,YANG X Y,LI Y Q,et al.LPS enhances platelets aggregation via TLR4,which is related to mitochondria damage caused by intracellular ROS,but not extracellular ROS[J].Cellular Immunology,2018,328:86-92.
[32]	SHIBATA T,MOTOI Y,TANIMURA N,et al.Intracellular TLR4/MD-2 in macrophages senses Gram-negative bacteria and induces a unique set of LPS-dependent genes[J].International Immunology,2011,23(8):503-510.
[33]	SUN S P,ZHANG C Y,LI H P,et al.Research progress on effect of γ-aminobutyric acid on LPS-mediated inflammation to the body[J].China Animal Husbandry & Veterinary Medicine,2018,45(8):2128-2134.
[34]	成果,廖瑛,周君,等.脂多糖诱导大鼠吸入性肺炎动物模型的建立[J].中国预防医学杂志,2019,20(5):396-399. CHENG G,LIAO Y,ZHOU J,et al.Establishment of animal model of aspiration pneumonia in rats induced by lipopolysaccharide[J].China Preventive Medicine,2019,20(5):396-399.(in Chinese)
[35]	姜文清,戚艳,沙若荷,等.PTEN在多烯磷脂酰胆碱下调LPS诱导巨噬细胞炎症反应中的抑制作用[J].中国比较医学杂志,2019,8:44-49. JIANG W Q,QI Y,SHA R H,et al.Inhibitory effect of PTEN on polyene phosphatidylcholine in downregula-ting LPS-induced macrophage inflammatory response[J].Chinese Journal of Comparative Medicine,2019,8:44-49.(in Chinese)
[36]	DIENZ O,EATON S M,BOND J P,et al.The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells[J].Journal of Experimental Medicine,2009,206(1):69-78.
[37]	施荣,王倩,韩丹,等.炎调方抑制LPS诱导人肺泡上皮细胞炎症因子释放机制研究[J].中国中医急症,2016,25(4):572-574. SHI R,WANG Q,HAN D,et al.Mechanism of Yantiaofang inhibiting LPS induced inflammatory cytokines release in human alveolar epithelial cells[J].Journal of Emergency in Traditional Chinese Medicine,2016,25(4):572-574.(in Chinese)
[38]	李黛,刘云峰,梁栋,等.二甲双胍对LPS诱导THP-1细胞所产生的相关炎症因子的影响[J].中西医结合心脑血管病杂志,2014,12(6):737-739. LI D,LIU Y F,LIANG D,et al.The effect of metformin on the related inflammatory factors produced by LPS induced THP-1 cells[J].Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease,2014,12(6):737-739.(in Chinese)
[39]	闫宝琪.黄藤素对LPS诱导的子宫内膜上皮细胞炎症反应的抗炎作用及机制研究[D].北京:中国农业科学院,2017. YAN B Q.Study on anti-inflammation effect and anti-inflammatory mechanism in LPS induced endometrial epithelial cells of palmatine[D].Beijing:Chinese Academy of Agricultural Sciences,2017.(in Chinese)
[40]	LIU S J,SHI Y,LIU C,et al.The upregulation of pro-inflammatory cytokines in the rabbit uterus under the lipopolysaccaride-induced reversible immuno-response state[J].Animal Reproduction Science,2017,176:70-77.
[41]	CHEN C,HE Z B,ZHANG M,et al.Expression pattern of Toll-like receptors (TLRs) in different organs and effects of lipopolysaccharide on the expression of TLR 2 and 4 in reproductive organs of female rabbit[J].Developmental & Comparative Immunology,2014,46(2):341-348.
[42]	邝晓娇.丹参水提物对LPS诱导子宫内膜上皮细胞炎症的抗炎机制研究[D].北京:中国农业科学院,2015. KUANG X J.Study on anti-inflammatory mechanism of Salvia miltiorrhiza extract against endometrium epithelial cells inflammation induced by LPS[D].Beijing:Chinese Academy of Agricultural Sciences,2015.(in Chinese)
[43]	LE C C,BEIGNEUX A P,HUANG J,et al.Regulation of lipopolysaccharide-induced tumor necrosis factor production by cyclosporin A in mice primed with muramyl dipeptide[J].FEMS Immuno-logy & Medical Microbiology,1995,11(4):297-305.
[44]	胡俊锋,夏雪梅,李殿明,等.环孢菌素A对脂多糖诱导的小鼠急性肺损伤的保护作用[J].中国应用生理学杂志,2011,27(1):120-123. HU J F,XIA X M,LI D M,et al.The effect of cyclosporine A on lipopolysaccharide-induced acute lung injury in mice[J].Chinese Journal of Applied Physiology,2011,27(1):120-123.(in Chinese)
[45]	GE Y N,XU Y,SUN W J.The molecular mechanisms of the effect of dexamethasone and cyclosporin A on TLR4/NF-κB signaling pathway activation in oral lichen planus[J].Gene,2012,508:157-164.
[46]	郑敬.钙调神经磷酸酶/活化T细胞核因子信号通路在大鼠主动脉钙化中的作用[D].石家庄:河北医科大学,2009. ZHENG J.Role of calcineurin/nuclear factor of activated T cells pathway in rat aorta calcification[D] Shijiazhuang:Hebei Medical University,2009.(in Chinese)
[47]	STEFFAN H,NEIL C,LUIKA T,et al.The mechanism of action of cyclosporin A and FK506[J].Clinical Immunology and Immunopathology,1996,80(3):40-45.
[48]	方兰艳,黎燕,郭顺星,等.以活化T细胞核因子为靶点的免疫抑制剂的研究进展[J].国际药学研究杂志,2002,29(2):87-91. FANG L Y,LI Y,GUO S X,et al.Research progress of immunosuppressants targeting activated T-cell nuclear factor[J].Foreign Medical Sciences.Section of Pharmacy,2002,29(2):87-91.(in Chinese)
[49]	迟秀文.环孢素A对正常人PBMCs产生IFN-γ的抑制作用及其机制的研究[D].广州:中山大学,2006. CHI X W.Study on the inhibitory effect of cyclosporine A on IFN-γ production of PBMCs and its mechanism[D].Guangzhou:Zhongshan University,2006.(in Chinese)
[50]	侯健,孔宪涛.环孢菌素A和FK506免疫抑制的分子机制[J].国外医学.药学分册,1993,4:193-195. HOU J,KONG X T.Molecular mechanism of immunosuppression of cyclosporin A and FK506[J].Foreign Medical Sciences.Section of Pharmacy,1993,4:193-195.(in Chinese)
[51]	陈燕,严明山,魏群.钙调神经磷酸酶和NFAT在T细胞活化中的作用[J].国外医学免疫学分册,1999,4:3-5. CHEN Y,YAN M S,WEI Q.Role of calcineurin and NFAT in T cell activation[J].Foreign Medical Sciences Section of Immunology,1999,4:3-5.(in Chinese)
[52]	HARISH B M,SARASWATHI R,DEVARAJU K S,et al.Molecular docking study of auto inhibitory domain fragments to calcineurin A[J].International Journal of Scientific & Engineering Research,2014,5(1):602-611.

Effect of CsA on the Expression of IL-1β and IL-6 in Endometrial Stomas Cells Induced by LPS

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 52

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHONG Yueyun, YANG Shuzhan, CHEN Feiyue, LU Zhier, LI Bingxin, LI Wanyan, TIAN Yunbo, HUANG Yunmao, XU Danning, CAO Nan. Effects of LPS on Apoptosis of Goose Embryo-derived Hepatocytes ROS/p38/MAPK Signaling Pathway [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2224-2232.
[2]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[3]	FU Jingcheng, HE Junhui, TONG Jiang, WU Wei, GUO Shuang, YANG Yanbin, HAN Yingqian, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Roles of HO-1 in Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 807-816.
[4]	GAO Jiahao, QIAO Yanjie, LIAN Kexun, WANG Mengmeng, GU Xinli, SHAO Yongbin. Effects of Glycyrrhizic Acid on Immune Performance of Lipopolysaccharide-stressed Yellow Feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3419-3427.
[5]	TANG Lin, ZHANG Junfang, WANG Ying, SUN Bin, WANG Enze, SHIN Jongsuh, GUO Panpan, JIN Xin, YAN Changguo, LI Xiangzi, LI Qiang. Anti-apoptotic Effect of Canine Adipose-derived Mesenchymal Stem Cells on Endoplasmic Reticulum Stress Model of Severe Acute Pancreatitis in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1840-1851.
[6]	ZHANG Yuzhu, YU Chao, ZHENG Huihua, TANG Xinyue, HUANG Ronglei, XIE Guanghong. Mechanism Study of Protocatechuic Acid on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1585-1591.
[7]	MA Sijia, LIU Yuan, WANG Xuzhong, LI Tingting, DUAN Xing, YANG Songbai, SONG Dan, LI Xiangchen. Effects of Melatonin on the Expression of Inflammatory Factors in Skeletal Muscle Satellite Cells Stimulated by LPS in Tan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3378-3386.
[8]	WANG Qian, WANG Manli, NING Nan, WANG Shuaijie, TAN Zihan, WANG Lei, ZHAO Di, ZHANG Qian, HOU Yongqing. Effect of N-acetylcysteine on Immune Response and Intestinal Mucosal Energy Status in Piglets Challenged with Lipopolysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 3996-4003.
[9]	WU Qin, ZHANG Xingxing, GU Xiaoxiao, TAOQIAO Xiaoci, HUANG Xin, HAN Mengli, WU Tongzhong, ZHOU Xia, ZHONG Fagang. Study on Serotyping and Detection of Virulence-associated Genes of Pasteurella multocida Isolated from Bovine [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1910-1920.
[10]	TAN Ya, GAN Mailin, FAN Yuan, WANG Jing, ZHANG Xiong, HUANG Bo, ZHU Li, SHANG Yishun, SHI Kaizhi. A Protective Effects of Fagopyrum dibotrys on Lipopolysaccharide-induced Intestinal Inflammation in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 597-604.
[11]	FENG Yan, NI Hemin, GUO Yong, SHENG Xihui, QI Xiaolong, XING Kai, WANG Xiangguo. Effect of Lipopolysaccharide on Expression of Uterine Receptive Factors in Dairy Cow Endometrial Epithelial Cells Cultured in vitro [J]. , 2019, 46(8): 2288-2295.
[12]	SONG Zhuan, ZHAO Guangyu, DONG Yi, JI Changzheng, LI Siyuan, WANG Lei, YI Dan, HOU Yongqing. Effect of Compound Plant Essential Oils on Structure of Intestinal Mucosa and Antioxidant Capacity of Weaned Piglets Challenged with Lipopolysaccharide [J]. , 2019, 46(3): 684-689.
[13]	XU Haiwang, YU Kui, WU Mengjun, LIU Wenkai, LI Peng, YI Dan, WANG Lei, HOU Yongqing. The Effect of Compound Plant Essential Oil on LPS-induced Immune-stressed Weaned Pigs [J]. , 2018, 45(8): 2197-2203.
[14]	LIU Bo, SHEN Yuan, MAO Wei, GAO Rui-feng, CAO Jin-shan. Effect of Prostaglandin E₂ and Prostaglandin F_2α on Cyclooxygenase-1 and Cyclooxygenase-2 Expression in Lipopolysaccharide-treated Bovine Endometrial Epithelial Cells [J]. , 2017, 44(9): 2716-2723.
[15]	LUO You, WU Xin-tong, PANG Xin-xin, XIAO Ke, PENG Ke-mei, SONG Hui. Study on the Building Conditions of Acute Lung Injury in Mice [J]. , 2017, 44(8): 2269-2276.