柑橘提取物对肉鸡运输应激后血浆生化指标、激素水平和抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2020.09.014

Abstract

Abstract: In order to study the effects of citrus extract on the plasma biochemical indexes,hormone level and antioxidant function of broilers after transport stress,a total of 360 twenty-eight-day-old fast-growing Yellow feather broilers were randomly divided into two groups (6 replicates per group,30 per replicate).The control group was fed basic diet without antibiotics,the experimental group was fed basic diet supplemented with 10 mg/kg citrus extract.At 63 days,six chickens were selected from each replicate for transport stress test for 0,2,and 4 h,respectively,and plasma sampling were conducted after transportation.The results showed that:① For glucose,cholesterol and urea nitrogen,there was a significant interaction between food treatment and transportation time (P<0.05).Compared with the control group,the contents of urea nitrogen and cholesterol in the plasma of broilers in the experimental group were significantly lower than that in the control group,and the content of glucose in the plasma of broilers in the experimental group was significantly higher than that in the control group (P<0.05).Transport stress significantly affected the content of urea nitrogen,cholesterol and glucose in the plasma of broilers in the control group,after transportation,the content of urea nitrogen and cholesterol increased significantly,while the content of glucose decreased significantly (P<0.05).② For corticosterone,triiodothyronine and thyroxine,there was significant interaction between food treatment and transport time (P<0.05).Compared with the control group,the content of corticosterone in the plasma of broilers in the experimental group was significantly lower than that in the control group,while the content of triiodothyronine and thyroxine in the experimental group was significantly higher than that in the control group (P<0.05).Transport stress significantly affected the content of corticosterone,triiodothyronine and thyroxine in the plasma of the control group,after transportation,the content of corticosterone in the plasma of the broiler significantly increased,while the content of triiodothyronine and thyroxine significantly decreased (P<0.05).③ For glutathione peroxidase,there was a significant interaction between food treatment and transportation time (P<0.05).Compared with the control group,the glutathione peroxidase activity in the plasma of broilers in the experimental group was significantly higher than that in the control group (P<0.05);Transport stress significantly affected the activity of glutathione peroxidase in the plasma of broilers in the control group.With the prolongation of transport time,the activity of glutathione peroxidase in the plasma of broilers decreased significantly (P<0.05).Therefore,transportation stress affected the metabolism level,hormone secretion and antioxidant function of Yellow feather broiler.Adding citrus extract to the diet could improve the metabolism level,regulate hormone secretion and improve antioxidant capacity,and alleviate the impact of transportation stress on Yellow feather broiler.

Key words: citrus extract; broiler; transport stress; hormone; antioxidant

CLC Number:

S816.7

LI Zhenming, YU Miao, CUI Yiyan, RONG Ting, LIU Zhichang, DU Zongliang, MA Xianyong. Effects of Citrus Extract on Plasma Biochemical Indexes,Hormone Levels and Antioxidant Function of Broilers after Transport Stress[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2816-2823.

References

[1] MIRANDA-DE LA LAMA G C,VILLARROEL M,MARIA G A.Livestock transport from the perspective of the pre-slaughter logistic chain:A review[J].Meat Science,2014,98(1):9-20.
[2] PERAI A H,KERMANSHAHI H,MOGHADDAM H N,et al.Effects of chromium and chromium+vitamin C combination on metabolic,oxidative,and fear responses of broilers transported under summer conditions[J].International Journal of Biometeorology,2015,59(4):453-462.
[3] 赵泮峰.连翘提取物缓解不同应激原诱导的肉仔鸡生长抑制及氧化损伤的研究[D].北京:中国农业大学,2016. ZHAO P F.Effects of forsythia suspense extract on alleviating growth lihibition and oxidative injury in broilers induced by different stimuli[D].Beijing:China Agricultural University,2016.(in Chinese)
[4] 张林,张海军,岳洪源,等.运输应激对畜禽的影响及其应对措施[J].家畜生态学报,2009,2:106-109. ZHANG L,ZHANG H J,YUE H Y,et al.Effects of transportation stress on livestock and alleviating strategy[J].Acta Ecologiae Animalis Domastici,2009,2:106-109.(in Chinese)
[5] 李绍钰,魏凤仙,徐彬,等.环境应激对肉鸡的影响和对策[J].动物营养学报,2014,26(10):3114-3121. LI S Y,WEI F X,X B,et al.Effects of environmental stress on broilers and its countermeasures[J].Chinese Journal of Animal Nutrition,2014,26(10):3114-3121.(in Chinese)
[6] 张挺.止痢草油缓解运输应激及改善猪肉质的作用[D].武汉:华中农业大学,2016. ZHANG T.Effect of oregano essential oil on reducing transport stress and improving meat quality in pigs[D].Wuhan:Huazhong Agricultural University,2016.(in Chinese)
[7] YUAN D X,HUSSAIN T,TAN B,et al.The evaluation of antioxidant and anti-inflammatory effects of Eucommia ulmoides flavones using diquat-challenged piglet models[J].Oxidative Medicine and Cellular Longevity,2017,2017:8140962.
[8] 李贞明,余苗,崔艺燕,等.柑橘提取物对黄羽肉鸡器官指数,屠宰性能,肉品质和肌肉抗氧化指标的影响[J].动物营养学报,2019,31(8):3794-3800. LI Z M,Y M,CUI Y Y,et al.Effects of citrus extract on organ index,slaughtering performance,meat quality and muscle antioxidant activity of Yellow broilers[J].Chinese Journal of Animal Nutrition,2019,31(8):3794-3800.(in Chinese)
[9] 金鑫.山药多糖对小鼠的抗应激作用研究[J].河南农业科学,2015,44(1):143-145. JIN X.Anti-stress effect of chinese yam polysa-ccharides in mice[J].Journal of Henan Agricultural Sciences,2015,44(1):143-145.(in Chinese)
[10] 张彩虹,李文立,任慧英,等.酵母铬对热应激肉鸡生长性能和血清生化指标的影响[J].动物营养学报,2008,20(6):668-673. ZHANG C H,LI W L,REN H Y,et al.Effects of chromium yeast on growth performance and serum biochemical parameters in heat stressed broilers[J].Chinese Journal of Animal Nutrition,2008,20(6):668-673.(in Chinese)
[11] 陈志刚,雷红宇,李啟皓,等.黄酮类化合物在鸡饲料添加剂中的研究与应用进展[J].中国家禽,2015,37(14):49-52. CHEN Z G,LEI H Y,LI Q H,et al.Progress of research and application of flavonoids in chicken feed additives[J].China Poultry,2015,37(14):49-52.(in Chinese)
[12] 王书全,李丽.螺旋藻多糖抗疲劳作用研究[J].食品工业科技,2013,34(22):328-330. WANG S Q,LI L.Study on anti-fatigue effect of Spiru-lina polysaccharide[J].Science and Technology of Food Industry,2013,34(22):328-330.(in Chinese)
[13] 朱良,张石蕊,贺喜,等.茶籽提取物对生长猪生长性能及血清生化指标的影响[J].中国饲料,2010,17:12-14. ZHU L,ZHANG S R,HE X,et al.Effects of Camellia oleifera seed extract on growth performance and serum biochemical indices in growing pigs[J].China Feed,2010,17:12-14.(in Chinese)
[14] 胡贵丽,刘靖,刘勇强,等.博落回生物碱和茶籽多糖对黄羽肉鸡生长性能,血清生化指标及脂质过氧化的影响[J].动物营养学报,2018,30(11):4626-4634. HU G L,LIU J,LIU Y Q,et al.Effects of Macleaya cordata alkaloids and tea seed polysaccharide on growth performance,serum biochemical indexes and lipid peroxidation of Yellow-feathered broilers[J].Chinese Journal of Animal Nutrition,2018,30(11):4626-4634.(in Chinese)
[15] 栗敏,李杰,王盼盼.代谢有机酸水平对生长育肥猪生长性能及血清生化指标的影响[J].中国饲料,2014,5:11-14. LI M,LI J,WANG P P,Effects of metabolic organic acid supplemented in dietary on growth performance and serum biochemical indices in growing-finishing pigs[J].China Feed,2014,5:11-14.(in Chinese)
[16] 李留安,杜改梅,金天明.运输应激对动物机体功能影响的研究进展[J].黑龙江畜牧兽医,2010,9:40-41. LI L A,DU G M,JIN T M.Research Progress on the effect of transport stress on animal body function[J].Heilongjiang Animal Science and Veterinary Medicine,2010,9:40-41.(in Chinese)
[17] 王国艮,吴鑫,孟庆翔,等.运输应激对反刍动物机体功能及肉品质影响的研究进展[J].中国畜牧兽医,2017,44(3):755-760. WANG G G,WU X,MENG Q X,et al.Research progress on effect of transport stress on ruminants body function and meat quality[J].China Animal Husbandry & Veterinary Medicine,2017,44(3):755-760.(in Chinese)
[18] SAEKI K,YOU A,TAKAKU F.Cell-cycle-regulated phosphorylation of cAMP response element-binding protein:Identification of novel phosphorylation sites[J].Biochemical Journal,1999,338(Pt 1):49-54.
[19] 朱振.应激对北京鸭血液指标,肉品质及组织HSPs mRNA转录水平的影响[D].扬州:扬州大学,2014. ZHU Z.Effects of stress on blood parameters,meat quality and HSPs mRNA transcript levels in Pekin ducks[D].Yangzhou:Yangzhou University,2014.(in Chinese)
[20] NIJDAM E,DELEZIE E,LAMBOOIJ E,et al.Feed withdrawal of broilers before transport changes plasma hormone and metabolite concentrations[J].Poultry Science,2005,84(7):1146-1152.
[21] 张舒翔,康大成,张丽丽,等.运输时间对扬州鹅应激程度和肉品质的影响[J].食品科学技术学报,2018,36(6):66-72. ZHANG S X,KANG D C,ZHANG L L,et al.Effects of transport time on stress level and meat quality in Yang-zhou goose[J].Journal of Food Science and Technology,2018,36(6):66-72.(in Chinese)
[22] 翁恺麒,张扬,陈博雯,等.运输应激和宰前休息对肉鸭血液指标和肉品质的影响[J].中国家禽,2017,39(14):37-40. WENG K Q,ZHANG Y,CHEN B W,et al.Effects of transport stress and rest before slaughter on blood parameters and meat quality in meat ducks[J].China Poultry,2017,39(14):37-40.(in Chinese)
[23] 程博琳.葫芦巴总黄酮抗抑郁主要药效学研究[D].郑州:河南中医药大学,2016. CHENG B L.Intervention of fenugreek flavonoid on depression animal models[D].Zhengzhou:Henan University of Chinese Medicine,2016.(in Chinese)
[24] 陈明珠,陈静,黄幼霞,等.绿萼梅总黄酮对慢性应激抑郁模型大鼠抑郁行为的影响及机制研究[J].中国药房,2017,28(13):1758-1762. CHEN M Z,CHEN J,HUANG Y X,et al.Study on the effects and mechanism of total flavonoids from Armeniaca mume on depression in chronic stress depression model rats[J].China Pharmacy,2017,28(13):1758-1762.(in Chinese)
[25] SUN L L,GAO S T,WANG K,et al.Effects of source on bioavailability of selenium,antioxidant status,and performance in lactating dairy cows during oxidative stress-inducing conditions[J].Journal of Dairy Science,2019,102(1):311-319.
[26] 单强,马峰涛,金宇航,等.富铬酵母对热应激奶牛生产性能、血清指标及血浆和牛奶中微量元素含量的影响[J].动物营养学报,2020,32(5):2198-2208. SHAN Q,MA F T,JIN Y H,et al.Effects of chromium yeast on performance,serum indexes and plasma and milk trace element contents of heat-stressed dairy cows[J].Chinese Journal of Animal Nutrition,2020,32(5):2198-2208.(in Chinese)
[27] BHATTACHARYYA A,CHATTOPADHYAY R,MITRA S,et al.Oxidative stress:An essential factor in the pathogenesis of gastrointestinal mucosal diseases[J].Physiological Reviews,2014,94(2):329-354.
[28] 李贞明,张贝贝,余苗,等.植物提取物的生物学功能及其在肉鸡生产中的应用进展[J].广东农业科学,2019,6:110-117. LI Z M,ZHANG B B,YU M,et al.Biological functions of plant extracts and their application in broiler production[J].Guangdong Agricultural Sciences,2019,6:110-117.(in Chinese)

Effects of Citrus Extract on Plasma Biochemical Indexes,Hormone Levels and Antioxidant Function of Broilers after Transport Stress

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