玉米赤霉烯酮降解菌的分离、鉴定及其适宜降解条件的研究

doi:10.16431/j.cnki.1671-7236.2020.09.013

Abstract

Abstract: The aim of this study was to reduce zearalenone (ZEN) in feed by bio-degradation rapidly and effectively.A total of 70 samples of cow manure,sheep manure and soil were collected from different areas and then 164 strains were isolated from these samples.Cyclopentanone was used as the only carbon source to preliminarily screen the 164 strains isolated from the collected samples,and the second-screening was performed using zearalenone as the only carbon source.As a result,8 ZEN-degrading strains were obtained.The ZEN-degrading efficiency of 8 strains was determined by high performance liquid chromatography (HPLC),and a strain with high ZEN-degrading,namely NF-PJ-5 strain.The strain was identified as Sphingobacterium multivorum by 16S rDNA sequence comparison,phylogenetic tree construction,colony morphology observation and physiological and biochemical identification.The ability of NF-PJ-5 strain to degrade zearalenone at different temperature,different pH and different concentration of bacterial solution were studied.In addition,the mechanism of ZEN degradation by this strain was studied.The results showed that at 28-32 ℃,pH 6.5-7.5,and the concentration of bacterial solution above 2×10⁹ CFU/mL,the strain could maintain a high degradation rate.Besides,the strain could degrade 83% ZEN with an initial concentration of 10 μg/mL,after 48 h culture at 30 ℃,pH 7.0,and a shaking speed of 160 r/min.From the above,a highly efficient ZEN-degrading strain was screened in this study,and it was identified as Sphingobacterium multivorum.It was preliminarily speculated that the strain could degrade ZEN by producing extracellular enzyme,and the cell wall of the strain had a certain adsorption capacity for ZEN.

Key words: zearalenone; bio-degradation; Sphingobacterium multivorum; identification

CLC Number:

S816.3

JIN Bowen, XU Changchun, LI Gen, LI Xiaoyu, WANG Lili, LI Jibin, XU Yongping. Isolation,Identification and Its Suitable Degradation Conditions of a Zearalenone-degrading Bacteria[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2808-2815.

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Isolation,Identification and Its Suitable Degradation Conditions of a Zearalenone-degrading Bacteria

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