酵母多糖对空肠弯曲菌感染獭兔生产性能、载菌量和抗菌肽表达的影响

doi:10.16431/j.cnki.1671-7236.2020.04.015

Abstract

Abstract: This study was aimed to investigate the effect of yeast polysaccharide (YPS) supplementation on growth performance,Campylobacter jejuni(C.jejuni) carriage and antimicrobial peptides in the epithelial tissue of cecum and skin in Rex rabbits.Five treatments included control and C.jejuni challenge with the addition of YPS at 0,200,400 or 600 mg/kg of diet.The trial lasted for 35 days and C.jejuni challenge was occurred at first day of feeding trial.The results showed that C.Jejuni challenge worsened final body weight,feed intake,body weight gain and feed efficiency (P < 0.05),whereas YPS supplementation compensated body weight gain and feed efficiency (P < 0.05).C.jejuni populations in the cecal digesta,skin,liver and spleen were decreased in the treatments containing YPS (P < 0.05). C.jejuni induced mRNA upregulation of Cathelicidin,Galectin-3 and LEAP2 gene (P < 0.05),but had no effect on DEFA4 gene,and addition of YPS further upregulated the gene expression (P < 0.05). Linear and quadratic trends of the three doses of YPS were found on final body weight and body weight gain (P<0.05),linear trends on C.jejuni carriage in tissues of skin,spleen and liver (P<0.05),linear trends on Cathelicidin and Galectin-3 in tissues of cecum and skin (P<0.05),and quadratic trends on Galectin-3,LEAP2 and DEFA4 gene in the cecal epithelium (P<0.05).It was concluded that YPS could effectively control C.jejuni infection by decreasing C.Jejuni carriage and activing endogenous epithelial antimicrobial peptides,and had no negative effect on the growth performance of rabbits.

Key words: yeast polysaccharide; Campylobacter jejuni; Rex rabbit; growth performance; antimicrobial peptide

CLC Number:

S829.1

ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Yeast Polysaccharide on Growth Performance,Bacterial Load and Endogenous Antimicrobial Peptides in Rabbits Infected with Campylobacter jejuni Expression[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1099-1108.

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Effect of Yeast Polysaccharide on Growth Performance,Bacterial Load and Endogenous Antimicrobial Peptides in Rabbits Infected with Campylobacter jejuni Expression

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