肉鸡养殖场中耐药相关基因散播情况的调查研究

doi:10.16431/j.cnki.1671-7236.2020.03.033

Abstract

Abstract: To investigate resistance genes and their spread related genes in broilers,totally 273 broiler fecal samples were collected by random sampling from two broiler farms in Hebei province.A total of eight kinds of resistance genes and two kinds of spread related genes including bla_NDM,bla_OXA,bla_CTX-M,armA,fexA,cfr,mcr-1,qnrS,and int1,ISCR1 which closely related to the spread of resistance gene,were detected by Real-time PCR,respectively.According to the results,all of the genes were existed in the colaca swab samples with positive rate 4.03%-97.07%.The aminoglycoside-resistance gene,armA,showed the lowest positive rate,and resistance gene spread related gene,int1,showed the highest positive rate.More than two (including two) kinds of resistance genes were detected in 273 of samples.Three kinds of resistance genes co-existing was the most common.Four samples harbored only one resistance gene,and six harbored none.No sample harbored eight kinds of resistance genes.There were 41 resistance gene type profiles through data analysis.The common resistance gene type profiles included bla_CTX-M-fexA-mcr-1 and bla_OXA-bla_CTX-M-fexA-cfr-mcr-1-qnrS.Based on statistics analysis,the more resistance genes detected,the higher int1/ISCR1 gene positive rate exhibited in samples which carried more than two (including two) kinds of resistance genes.The coexistence of resistance genes bla_OXA,bla_CTX-M,fexA,cfr,mcr-1,qnrS with int1 gene showed statistical significance (P<0.05),respectively.And,the coexistence of resistance genes bla_NDM,bla_OXA,bla_CTX-M,fexA,cfr,mcr-1,qnrS with ISCR1 gene showed statistical significance (P<0.05),respectively.This study provided new Real-time PCR methods for drug resistance surveillance of animal husbandry and veterinary realms,and gave data support to drug resistance prevention and control in future.

Key words: broiler; resistance gene; gene spread; relationship

CLC Number:

S831

CHEN Xia, CHE Jie, LUO Pengjie, ZHANG Yunfei, ZHAO Xiaofei, YUAN Min, BAI Xuemei, LI Wenge, LI Juan. Investigation on the Spread of Resistance-related Genes in Broiler Farms[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 931-939.

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Investigation on the Spread of Resistance-related Genes in Broiler Farms

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