中草药复方防治雏鸡沙门氏菌病的研究

doi:10.16431/j.cnki.1671-7236.2020.03.031

Abstract

Abstract: In order to evaluate the effect of Chinese herbal medicine compound on the prevention and treatment of S.Enteritidis in chicken,a compound of Chinese herbal medicine complex consisting of 23 Chinese herbal medicines such as Coptidis rhizome,wintergreen,and white-headed medicinal herbs was selected for in vitro and in vivo antibacterial experiments in broiler chicken.The antibacterial diameter of the Chinese herbal medicine complex for the in vivo bacteriostatic experiment was 17.4 mm,indicating that the S.Enteritidis was highly sensitive to it.In in vitro antimicrobial experiment,low (0.1%),medium (0.3%),and high (0.5%) dose of the Chinese herbal medicine complex were used.After orally-infected with S.Enteritidis to the broiler,compared with the control group,the survival rates increased extremely significantly (P<0.01) and the feed to gain ratio (F/G) decreased significantly in three experimental groups (P<0.05).The average daily weight gain (ADG) of the middle and high dose groups were significantly higher (P<0.05),and the average daily feed intake (ADFI) of the middle dose group was significantly lower (P<0.05).The spleen index,white blood cell count (WBC),platelet count (PLT),IgA,IgG,IgM,and complement C4 levels in the three experimental groups were significantly lower (P<0.05),while the thymus,bursa of Fabricius indexes,red blood cell count (RBC),hemoglobin concentration (HGB),hematocrit (HCT),and platelet count (PCT) were significantly higher (P<0.05).The results of Real-time quantitative PCR showed that the gene expression levels of antibacterial peptide gene cathelicidins (CATH1,CATH2,CATH3) in spleen,thymus and bursa of Fabricius in three treatment groups were higher than that in control group.Among Chinese herbal medicine doses,the meddle dose (0.3%) had the best control effect.From above mentioned results,it was concluded that the combination of Chinese herbal medicine in the diet can reduce the mortality of chickens infected with S.Enteritidis,enhance growth performance,immunity and antibacterial activity of the chickens against S.Enteritidis.

Key words: Salmonella; Chinese herbal complex; growth performance; cathelicidins genes

CLC Number:

S853.74

LI Zhengtian, DOU Tengfei, LI Qihua, GE Changrong, JIA Junjing, ZHANG Xi. The Prevention and Treatment of a Chinese Herbal Medicine Compound for Salmonellosis in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 911-921.

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The Prevention and Treatment of a Chinese Herbal Medicine Compound for Salmonellosis in Chickens

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