China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (1): 131-139.doi: 10.16431/j.cnki.1671-7236.2020.01.016

• Genetics and Breeding • Previous Articles     Next Articles

Expression of Porcine DHRS4 Gene in Skeletal Muscle and Its Association with Growth Traits

ZHANG Leixia1, YANG Yanzhao1, LI Wentong2, LIU Ying2, ZHOU Rong2, YANG Yalan1, LI Kui2   

  1. 1. Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan University, Foshan 528231, China;
    2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2019-05-06 Online:2020-01-20 Published:2020-01-17

Abstract: In order to investigate the expression of porcine DHRS4 gene in skeletal muscle and its association with growth traits,this study analyzed the expression of DHRS4 gene in different types of skeletal muscles in Landrace pigs at 30,180 and 300 d.The single nucleotide polymorphism (SNP) of DHRS4 gene in Lansi White pig resource population were identified and genotyped by the Sequenom genotyping platform.The association of the SNP in DHRS4 gene was analyzed with growth traits,including feed gain ratio,average daily gain and average backfat thickness.The results showed that DHRS4 gene was expressed in breast,leg and dorsal muscles of Landrace pigs at 30,180 and 300 d.In breast and leg muscles,the expression of DHRS4 was significantly or extremely significantly higher at 30 d than that at 180 and 300 d (P<0.05;P<0.01),but there was no significant difference of dorsal muscles at different developmental stages (P>0.05).In addition,this study found 3 SNPs of DHRS4 gene in Lansi White pig resource population:rs334250151,rs342446613 and rs326982309.The association analysis results showed that rs334250151 loci was significantly associated with feed gain ratio,and the feed gain ratio of AA genotype individuals was significantly lower than that of TT genotype individuals (P<0.05).This results reveals that DHRS4 gene might be involved in porcine skeletal muscle development,and rs334250151 loci could be used as a new molecular marker for genetic improvement of growth traits in pig.

Key words: DHRS4 gene; SNP; growth traits; skeletal muscle

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