副乳头对奶牛生产性能的影响及其相关基因LGR5的遗传表达分析

doi:10.16431/j.cnki.1671-7236.2019.12.022

Abstract

Abstract: This study was aimed to investigate the effect of supernumerary teat (SNT) on the milk performance of dairy cows and the relationship between mammogenesis gene LGR5 and supernumerary teat.Dairy herd improvement (DHI) data of 325 supernumerary teat and 737 non-supernumerary teat dairy cows in the same born year were collected to compare the differences in milk performance.The differential expression of LGR5 gene between supernumerary teat and non-supernumerary teat dairy cows was performed by Real-time quantitative PCR.The genetic mutation of LGR5 gene was detected by direct sequencing method.The results showed that the daily milk yield,peak milk yield and lactation persistence of non-supernumerary teat dairy cows were better than that of supernumerary teat dairy cows,but did not reach the statistical significant level.The expression of LGR5 gene in supernumerary teat dairy cows was significantly higher than that in non-supernumerary teat dairy cows (P<0.05).3 SNPs (rs42849472,rs42849471 and rs42849470) were identified in LGR5 gene intron 1.The frequency of AA genotype of 3 SNPs in supernumerary teat dairy cows were extremely significantly higher than that in non-supernumerary teat dairy cows (P<0.01).The results suggested that LGR5 gene might be involved in the occurrence of supernumerary teat in dairy cows,and could be used as an effective candidate gene.

Key words: dairy cows; supernumerary teat; non-supernumerary teat; milk performance; LGR5 gene

CLC Number:

S813.3

WEN Hui, LUO Hanpeng, MI Siyuan, LIU Xueqin, WANG Yachun, XIAO Wei, YU Ying. Effect of Supernumerary Teat on Milk Performance and Genetic Expression Analysis of LGR5 Gene in Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3642-3649.

References

[1] BUTTY A M,FRISCHKNECHT M,GREDLER B,et al.Genetic and genomic analysis of hyperthelia in Brown Swiss cattle[J].Journal of Dairy Science,2017,100(1):402-411.
[2] PAUSCH H,JUNG S,EDEL C,et al.Genome-wide association study uncovers four QTL predisposing to supernumerary teats in cattle[J].Animal Genetics,2012,43(6):689-695.
[3] PALACIOS C,ABECIA J A.Supernumerary teat removal can be avoided in dairy sheep[J].Journal of Applied Animal Welfare Science,2014,17(2):178-182.
[4] BRKA M,REINSCH N,KALM E.Frequency and heritability of supernumerary teats in German Simmental and German Brown Swiss cows[J].Journal of Dairy Science,2002,85(7):1881-1886.
[5] CARMON K S,LIN Q,GONG X,et al.LGR5 interacts and cointernalizes with Wnt receptors to modulate Wnt/β-catenin signaling[J].Molecular and Cellular Biology,2012,32(11):2054-2064.
[6] WU X S,XI H Q,CHEN L.LGR5 is a potential marker of colorectal carcinoma stem cells that correlates with patient survival[J].World Journal of Surgical Oncology,2012,10:244-251.
[7] STEINHART Z,ANGERS S.Wnt signaling in development and tissue homeostasis[J].Development,2018,145(11):146-154.
[8] DE LAU W,BARKER N,LOW T Y,et al.LGR5 homologues associate with Wnt receptors and mediate R-spondin signalling[J].Nature,2011,476(7360):293-297.
[9] CLEVERS H.Wnt/β-catenin signaling in development and disease[J].Cell,2006,127(3):469-480.
[10] NUSSE R,CLEVERS H.Wnt/β-Catenin signaling,disease,and emerging therapeutic modalities[J].Cell,2017,169(6):985-994.
[11] PLAKS V,BRENOT A,LAWSON D A,et al.LGR5 expressing cells are sufficient and necessary for postnatal mammary gland organogenesis[J].Cell Reports,2013,3(1):70-78.
[12] URBANI C E,BETTI R.The significance of familial polythelia[J].Journal of American Academy of Dermatology,1995,32(4):687-688.
[13] JANG B G,KIM H S,CHANG W Y,et al.Expression profile of LGR5 and its prognostic significance in colorectal cancer progression[J].American Journal of Pathology,2018:188(10):2236-2250.
[14] MORGAN R G,MORTENSSON E,WILLIAMS A C.Targeting LGR5 in colorectal cancer:Therapeutic gold or too plastic[J].British Journal of Cancer,2018,118(11):1410-1418.
[15] 俞清,曾艺.粉红丝带引发的思考——Wnt信号通路在乳腺发育与乳腺癌变中的角色[J].科技导报,2016,20:5-13. YU Q,ZENG Y.Mammary development and breast cancer:A Wnt prospective[J].Science and Technology Review,2016,20:5-13.(in Chinese)
[16] ROELNUSSE.Wnt signaling in disease and in development[J].Cell Research,2005,15(1):28-32.
[17] CHEN Q,CAO H Z,ZHENG P S.LGR5 promotes the proliferation and tumor formation of cervical cancer cells through the Wnt/beta-catenin signaling pathway[J].Oncotarget,2014,5(19):9092-9105.
[18] 张书勤,马薇,丰小敏,等.LGR5和MIF在结直肠癌组织中的表达及其临床意义[J].中国临床研究,2017,30(5):596-599. ZHANG S Q,MA W,FENG X M,et al.Expressions and clinical significance of LGR5 and MIF in colorectal cancer tissues[J].Chinese Journal of Clinical Research,2017,30(5):596-599.(in Chinese)
[19] TIMMERMANS-SPRANG E P M,GRACANIN A,MOL J A.Molecular signaling of progesterone,growth hormone,Wnt,and HER in mammary glands of dogs,rodents,and humans:New treatment target identification[J].Frontiers in Veterinary Science,2017,4:53-65.
[20] SONG W,WANG R,JIANG W,et al.Hormones induce the formation of luminal-derived basal cells in the mammary gland[J].Cell research,2019,29(3):206-220.
[21] GANDHOKE C S,SYAL S K,SINGH H,et al.Dorsal accessory ectopic breast with polythelia——A marker of occult spinal dysraphism[J].Surgical Neurology International,2018,9:143-149.
[22] CUNHA G R.Role of mesenchymal-epithelial interactions in normal and abnormal development of the mammary gland and prostate[J].Cancer,2015,74(S3):1030-1044.
[23] 曲波,李庆章.激光共聚焦显微技术在乳腺细胞凋亡研究中的应用[J].东北农业大学学报,2009,40(3):121-125. QU B,LI Q Z.Application of laser scanning confocal microscope in apoptosis in mammary gland[J].Journal of Northeast Agricultural University,2009,40(3):121-125.(in Chinese)
[24] HOWARD B A,GUSTERSON B A.The characterization of a mouse mutant that displays abnormal mammary gland development[J].Mammalian Genome,2000,11(3):234-237.
[25] KANDA A,CHEN W,OTHMAN M,et al.A variant of mitochondrial protein LOC387715/ARMS2,not HTRA1,is strongly associated with age-related macular degeneration[J].Proceedings of the National Academy of Sciences,2007,104(41):16227-16232.
[26] KIMCHI-SARFATY C,OH J M,KIM I W,et al.A ‘silent’ polymorphism in the MDR1 gene changes substrate specificity[J].Science,2007,315(5811):525-528.
[27] NACKLEY A G,SHABALINA S A,TCHIVILEVA I E,et al.Human catechol-O-methyltransferase haplotypes modulate protein expression by altering mRNA secondary structure[J].Science,2006,314(5807):1930-1933.

Effect of Supernumerary Teat on Milk Performance and Genetic Expression Analysis of LGR5 Gene in Dairy Cows

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