汤阴北艾精油对鼠CYP450酶活性和表达的影响

doi:10.16431/j.cnki.1671-7236.2019.11.035

Abstract

Abstract: Effect of essential oil from Artemisia vulgaris on CYP450 isoforms activity and expression in mouse was detected in this study.Essential oil was extracted from Artemisia vulgaris by steam extraction and was detected by GC-MS.In order to study the effect of essential oil on CYP450 activity,SD rats were randomly divided into control group (CON) and essential oil group (EO).The rats were given 0.1% Tween-80 100 μL/g and 0.1% Tween-80 diluted essential oil (5%) 100 μg/g respectively by gavage.After gavage,20 μg/g probes were given.Cocktail probes were used to measure the concentrations of three probe at 5,10,20 and 30 min,and 1,2,3,6,12,24,36 and 48 h after administration.To study the essential oil on the expression of CYP450,the Kunming mice were randomly divided into two groups.The control group was administrated with 1% Tween-80 (100 μL/g) and the essential oil group with 5% essential oil (100 μg/g).The administration was given twice daily for 3 days.The mRNA and protein expression were tested by Real-time PCR and Western blotting.The results show that:①The major components of essential oil were Eucalyptol;3-Cyclohexen-1-ol,4-methyl-1-(1-methylethyl)-3;(+)-2-Carene,4-.alpha.-isopropenyl-2;m-Mentha-4,8-diene,(1S,3S)-(+)-;Benzoic acid,2,4,6-trimethyl-,2,4,6-trimethylphenyl ester.②Pharmacokinetics of Phenacetin(CYP1A2 substrate),dextromethorphan(CYP2D6 substrate) and chlorzoxazone(CYP2E1 substrate) were analyzed.The results suggested that the essential oil could inhibit the activities of CYP2D6 and CYP2E1,while,had no effect on the activity of CYP1A2.③Real-time PCR and Western blotting results showed that essential oil significantly decreased the CYP2E1 and CYP2D6 expression.In conlusion,essential oil from Artemisia vulgaris could inhibit the activities and expressions of CYP2E1 and CYP2D6.It suggested that the essential oil from Artemisia vulgaris could not be taken together with the drugs in which metabolic pathway CYP2D6 and CYP2E1 were the main factors.The results of this study could provide data basis for the rational use of essential oil from Artemisia vulgaris.

Key words: Artemisia vulgaris; essential oil; pharmacokinetics; CYP450

CLC Number:

S853.74

JIANG Zhihui, ZHANG Jingmiao, QU Yicheng, ZHANG Shouquan, CHANG Xuemei, GUO Xiao. Effect of Essential Oil from Artemisia vulgaris on CYP450 Isoforms Activity and Expression[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3440-3448.

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Effect of Essential Oil from Artemisia vulgaris on CYP450 Isoforms Activity and Expression

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