体外产气法筛选基于真姬菇菌糟碱贮的山羊日粮配方

doi:10.16431/j.cnki.1671-7236.2019.07.010

Abstract

Abstract:

In this experiment,6 healthy Chuanzhong Black goats ((25±2.5) kg) were selected and rumen fistula was installed for rumen fluid collection.The ANKOM RFS in vitro gas production system was used to select the diet formula based on compound alkali storage of spent mushroom substrate.The test formula was prepared according to the nutrient requirement of NRC goats.The addition ratio of the compound alkali storage of spent mushroom substrate was 0,20%,30%,40% (CK,SMS2,SMS3,SMS4),and each level was repeated 3 times.The gas production (GP) at 0,6,12,18,24,30,36,42 and 48 h was measured,and the volatile fatty acids (VFA),microbial protein (MCP),ammonia nitrogen (NH₃-N),pH and in vitro true digestibility (IVTD) in fermentation broth at 48 h were determined.The results showed that the GP_{48 h},IVTD,MCP and TVFA of the experimental groups SMS2,SMS3 and SMS4 compared with control group,were increased by 6.87%(P<0.01),9.40%(P<0.01),4.22%(P<0.05);10.98%(P<0.01),16.11%(P<0.01),18.17%(P<0.01);4.01%(P<0.05),8.59%(P<0.05),10.08%(P<0.05) and 12.96%(P<0.05),24.93%(P<0.01),31.09%(P<0.01),respectively.The pH of four groups ranged from 6.66 to 6.70,NH₃-N ranged from 16.96 to 18.45 mg/100 mL,and A/P ranged from 3.68 to 4.33,they were all within the appropriate range.In conclusion,compound alkali storage of spent mushroom substrate could replace part of the roughage,and the recommended dosage was 30% for the fattening goat diet.

Key words: goat; spent mushroom substrate; alkali storage; gas production in vitro; formula screening

CLC Number:

S816.8

XU Jincong, PAN Shuangzi, MIAO Jing, HE Xiangbo, LIANG Xuewu, GAN Qianfu. Goat Formula Screening Based on Spent Mushroom (Hypsizygus marmoreus) Substrate Alkali Storage by In vitro Gas Production[J]. , 2019, 46(7): 1962-1968.

References

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Goat Formula Screening Based on Spent Mushroom (Hypsizygus marmoreus) Substrate Alkali Storage by In vitro Gas Production

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