广西部分地区猪链球菌血清型及其毒力因子流行性调查分析

doi:10.16431/j.cnki.1671-7236.2019.04.019

Abstract

Abstract:

To investigate the distribution of major serotypes and virulence factors of Streptococcus suis (SS) strains in parts regions of Guangxi,116 samples of suspected streptococcal infections (brain,lung,lymph nodes,etc.) collected from different swine farms from January to June in 2018 were examined in this study.The serotypes and some virulence genes were identified by bacterial isolation,morphological observation,PCR amplification.The positive rate of Streptococcus suis in 116 samples was 27.59% (32/116).The main serotypes of positive samples were SS2 (40.63%,13/32) and SS9 (43.75%,14/32),and other serotypes accounted for 15.63% (5/32).The test results of virulence factors demonstrated that the detection rates of SLY,MRP,EPF and SBP2' factors were 81.25% (26/32),59.38% (19/32),50.00% (16/32) and 71.88% (23/32),respectively.Among the 32 strains of Streptococcus suis,SLY⁺MRP⁺EPF⁺SBP2'⁺(13 strains),SLY⁺MRP^-EPF^-SBP2'^-(5 strains) and SLY⁺MRP⁺EPF^-SBP2'⁺(5 strains) were the main virulence genotypes.And SS2 could be detected three or more virulence genes.SS9 was the prevalent type in Yulin and Liuzhou.SS2 was the prevalent type in Nanning and Baise.It was discovered that there were differences in the distribution of virulence factors in the four regions of Guangxi.The distribution of streptococcal virulence factors in different serotypes or the same serotype was different,and the detection rate of virulence gene of SS2 was higher than other serotypes.This study could provide a theoretical basis for the future research of Streptococcus suis vaccine and pathogenesis,and provide guidance for the selection of serotypes of Streptococcus suis vaccine in Guangxi.

Key words: Streptococcus suis; serotype; virulence gene; prevention and control

CLC Number:

S852.61

SHI Dali, GE Chenling, ZHU Yuanzhi, XIN Jialiang, GE Qiang, YUAN Jingzhi, WANG Xiaoye, LI Xun, ZHENG Min, WEI Xiankai. Investigation on the Prevalence of Serotypes and Virulence Factors of Streptococcus suis in Some Areas of Guangxi[J]. , 2019, 46(4): 1116-1126.

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Investigation on the Prevalence of Serotypes and Virulence Factors of Streptococcus suis in Some Areas of Guangxi

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