表达Ⅰ型耐热肠毒素的重组大肠杆菌对7日龄仔猪小肠功能的影响

doi:10.16431/j.cnki.1671-7236.2019.02.012

Abstract

Abstract:

This experiment was aimed to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa) on small intestinal function in 7 days old piglets.Twenty-four 7 days old piglets were randomly divided into four treatments of control,STa,LMG194 and K88 groups,which were fed with artificial milk throughout the experiment.Pre-feeding period was 4 d.STa,LMG194 and K88 groups were orally administered with 2×10⁹ CFU E.coli LMG194-pBAD-STa,LMG194 and K88 twice in the morning and evening on the 5th day,respectively.The control group was given the same amount of normal saline.On the 7th morning of the experiment,each group was given D-xylose and blood was collected.Then they were slaughtered and the samples of jejunum and ileum tissue were collected,blood routine indicators,the content of plasma D-xylose,the activity of diamine oxidase (DAO),and the expression of absorption and transport related genes and protein were measured.The results showed that compared with control group,the neutrophils and neutrophil ratio were significantly decreased in blood,the lymphocyte ratio was significantly increased in STa group (P<0.05),the neutrophil ratio was significantly decreased in blood,the lymphocyte ratio was significantly increased in LMG194 and K88 groups (P<0.05);The content of D-xylose in plasma was significantly decreased in STa and K88 groups (P<0.05);The activity of DAO in plasma was significantly increased in STa,LMG194 and K88 groups (P<0.05).In jejunum,compared with control group,the expression levels of AQP8, AQP10,KCNJ13 and b^0,+AT genes were significantly reduced in STa and LMG194 groups (P<0.05),the expression levels of AQP8 and AQP10 genes were significantly increased in K88 group (P<0.05).In ileum,the expression level of AQP8 gene was significantly reduced,the expression levels of AQP10 and SGLT-1 genes were significantly increased in STa group (P<0.05).The expression level of AQP8 gene was significantly increased in LMG194 and K88 groups (P<0.05),the expression levels of AQP10,KCNJ13,b^0,+AT and SGLT-1 genes were significantly reduced in K88 group (P<0.05).The relative expression level of HSP70 was significantly increased in STa,LMG194 and K88 groups (P<0.05).These results suggested that recombinant E.coli expressing STa could lead to inflammation in small intestinal,absorption and transfer capacity were decreased in 7 days old piglets.

Key words: heat-stable enterotoxin (STa); recombinant E.coli; piglets; intestinal

CLC Number:

S828

WU Mengjun, LI Siyuan, JI Changzheng, YU Kui, DONG Yi, ZHAO Guangyu, ZHAO Di, WU Tao, HOU Yongqing. Effects of Recombinant E.coli Expressing Heat-stable Enterotoxin on Small Intestinal Function in 7 Days Old Piglets[J]. , 2019, 46(2): 424-432.

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Effects of Recombinant E.coli Expressing Heat-stable Enterotoxin on Small Intestinal Function in 7 Days Old Piglets

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