›› 2019, Vol. 46 ›› Issue (1): 305-312.doi: 10.16431/j.cnki.1671-7236.2019.01.036

• Basic Veterinary Medicine • Previous Articles     Next Articles

Studies on the Pharmacological Effects of Compound Myrobalan Oral Liquid Against Escherichia coli Diarrhea in Calves

HAN Yuwen1, LI Zijia1, YU Wenhui1, WANG Na1, JIANG Xiaowen1, HAO Jingyou1,2   

  1. 1. College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China;
    2. Harbin Ludasheng Animal Pharmaceutical Limited Liability Company, Harbin 150030, China
  • Received:2018-05-07 Online:2019-01-20 Published:2019-01-19

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Abstract:

The aim of the experiment was to study the pharmacological effects of compound Myrobalan oral liquid against Escherichia coli (E.coli) diarrhea in calves.We used neutralize enterotoxin test,anti-exudation test,antipyretic test,analgesic test,intestinal peristalsis test and immunomodulation test to verify the pharmacological effects of compound Myrobalan oral liquid.The results showed that in the neutralize enterotoxin test,there was extremely significant difference between compound Myrobalan oral liquid and negative control groups (P < 0.01).In the anti-exudation test,all drug groups could reduce the exudation of Evans blue to varying degrees,the inhibition rates of the aspirin group,compound Myrobalan oral liquid high,medium and low dose groups were 58.1%,42.8%,31.6% and 14.9%,respectively.Among them,the compound Myrobalan oral liquid low dose group was significantly different from blank group (P < 0.05),while other groups were extremely significantly different from blank group (P < 0.01).The compound Myrobalan oral liquid medium and low dose groups had extremely significant difference compared with aspirin group (P < 0.01),but the compound Myrobalan oral liquid high dose group had no significant difference(P > 0.05).In the antipyretic test,the high-dose group of compound Myrobalan oral liquid quickly inhibited the increase of body temperature 1 h after administration,and the increase of body temperature was inhibited 2 h after the administration in medium dose group;3 h after the administration,the body temperature was significantly reduced in high and medium dose groups compared with model group;The difference between low dose and model groups was not significant.In the anti-analgesic test,there were extremely significant differences in aspirin group,compound Myrobalan oral liquid high and medium dose groups compared with blank group (P < 0.01),the writhing inhibition rates were 64.55%,52.73% and 32.73%,respectively,the pain latency were 9.59,6.33 and 5.74 min;And there was no significant difference in low dose group (P > 0.05).In the intestinal peristalsis test,compared with blank group,compound Myrobalan oral liquid high dose and atropine sulphate groups had obviously inhibitory effect on intestinal peristalsis (P < 0.01),and the promotion rates were 44.18% and 52.55%,respectively;The effect of medium dose group of compound Myrobalan oral liquid was significant different (P < 0.05),low dose group of compound Myrobalan oral liquid was not significant (P > 0.05).In the immunomodulation test,the white blood cell (WBC) count,spleen and thymus organ indexes in model group were significantly lower than those in blank group (P < 0.05),and the WBC count,spleen and thymus organ indexes in compound Myrobalan oral liquid group were significantly higher than those in model group on the 14th day (P < 0.05).In conclusion,compound Myrobalan oral liquid had neutralize enterotoxin,anti-exudation of tissue fluid,antipyretic,analgesia and inhibitory effect on intestinal peristalsis of E.coli diarrhea,and could enhance the immune function and other pharmacological effects.

Key words: compound Myrobalan oral liquid; Escherichia coli; diarrhea; pharmacological effects

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