添加不同水平糖蜜对全株“张杂谷”青贮体外瘤胃发酵特性和营养物质降解率的影响

doi:10.16431/j.cnki.1671-7236.2018.10.012

Abstract

Abstract:

The study was aimed to explore the effects of different molasses levels on in vitro ruminal fermentation characteristics and nutrient degradation of whole "Zhang Hybrid Millet" silage.Three Holstein dairy cows with permanent rumen fistula and similar body condition were chosen and the rumen fluid was collected.0(control group),2%,4% and 6% molasses were added to whole "Zhang Hybrid Millet" silage for 60 d fermentation at indoors temperature.After that the quality of whole "Zhang Hybrid Millet" silage were valued.The results showed that:① The DM of 4% molasses group was significantly higher than that of 2% and 6% molasses groups (P < 0.05),which was not significantly different with control group (P > 0.05).There was no significant difference of CP content among the groups (P > 0.05);The content of NDF of 4% molasses group was significantly lower than that of control group and 2% molasses group (P < 0.05),while the ADF was significantly lower than other group (P < 0.05).② After adding 4% molasses,DMD was significantly higher than that in control group and 2% molasses group (P < 0.05).The CPD and NDFD of 4% molasses group was significantly higher than that of control group and 6% molasses group (P < 0.05),while the ADFD was significantly higher than that in control group (P < 0.05).③Compared with the control group,ME,NE_L and GP_{72 h} of molasses adding groups were increased (P > 0.05).And the C value of 4% molasses group was significantly lower than that of control group (P < 0.05) when the gas production volume reached the theoretical maximum yield of 1/2.④There was no significant difference of pH,NH₃-N,MCP,TVFA,NGR,acetic acid,propionic acid,butyric acid,valeric acid and branched-chain fatty acid between molasses adding groups and control group (P > 0.05),while the fermentation parameters of 4% molasses group were better.Under the condition of this study, the optimum level of molasses was 4%.

Key words: molasses; whole “Zhang Hybrid Millet” silage; in vitro rumen fermentation; kinetic parameters of gas production

CLC Number:

S816.5⁺3

WU Jincai, LIU Tingting, YUE Chunwang, GUO Jinshuang, ZHAO Zhihai, FAN Guangyu, HAO Fengyong, KONG Xianghao, SUN Maohong. Effects of Different Molasses Levels on in vitro Ruminal Fermentation Characteristics and Nutrient Degradation of Whole “Zhang Hybrid Millet” Silage[J]. , 2018, 45(10): 2752-2760.

References

[1] 张亚格,字学娟,李茂,等.绿汁发酵液对柱花草青贮营养成分及品质的影响[J].中国畜牧兽医,2016,43(6):1475-1480.ZHANG Y G,ZI X J,LI M,et al.Effects of previously fermented juice on the nutrient composition and quality of stylo silage[J].China Animal Husbandry & Veterinary Medicine,2016,43(6):1475-1480.(in Chinese)
[2] 叶均安,刘建新,叶宏伟.氨化稻草日粮补饲青贮紫云英对培育牛生长性能的影响[J].中国饲料,1995,17:24-25.YE J A,LIU J X,YE H W.Effects of ammoniated rice straw supplementation with Ziyunying silage on the growth performance of cattle[J].China Feed,1995,17:24-25.(in Chinese)
[3] 郭金双,赵广永,杨雅芳,等.添加蔗糖对大麦青贮品质及中、酸性洗涤纤维瘤胃降解率的影响[J].中国畜牧杂志,2000,36(4):18-20.GUO J S,ZHAO G Y,YANG Y F,et al.Effects of sugar supplementation on the quality of barley silage and NDF and ADF degradation in the rumen of cattle[J].Chinese Journal of Animal Science,2000,36(4):18-20.(in Chinese)
[4] JONES B A,SATTER L D,MUCK R E.Influence of bacterial inoculant and substrate addition to lucerne ensiled at different dry matter contents[J].Grass & Forage Science,1992,47(1):19-27.
[5] 董妙音,王曙阳,姜伯玲,等.添加不同的青贮菌剂对甜高粱青贮品质的影响[J].饲料工业,2016,1:28-31.DONG M Y,WANG S Y,JIANG B L,et al.Effects of different silage inoculants on the quality of sweet sorghum silage[J].Feed Industry,2016,1:28-31.(in Chinese)
[6] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.FENG Y L.Ruminant Nutrition[M].Beijing:Science Press,2004.(in Chinese)
[7] 杨红建,宋正河,祝仕平,等.一种发酵微量气体产生量数据自动采集存储装置及方法[P].2007.YANG H J,SONG Z H,ZHU S P,et al.An automatic data collection and storage device for the production of trace gas in fermentation[P].2007.(in Chinese)
[8] MENKE K H,STEINGASS H.Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J].Animal Research and Development,1988,28:47-55.
[9] ZHAO G Y,XUE Y,ZHANG W.Relationship between in vitro gas production and dry matter and organic matter digestibility of rations for sheep[J].Journal of Animal and Feed Sciences,2007,16(2):229-234.
[10] 张丽英.饲料分析及饲料质量检测技术[M].第3版.北京:中国农业大学出版社,2007.ZHANG L Y.Feed Analysis and Feed Quality Testing Technology[M].3rd edition.Beijing:China Agricultural Press,2007.(in Chinese)
[11] MAKKAR H P S,SHARMA O P,DAWRA R K.Simple determination of microbial protein in rumen liquor[J].Journal of Dairy Science,1982,65:2170-2173.
[12] GROOT J C J,CONE J W,WILLIAMS B A,et al.Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds[J].Animal Feed Science and Technology,1996,64:77-89.
[13] CUI J H, YANG H J,YU C Q,et al.Effect of urea fertilization on biomass yield,chemical composition, in vitro rumen digestibility and fermentation characteristics of forage oat straw in Tibet of China[J].Journal of Agricultural Science,2016,154(5):914-927.
[14] 丁武蓉,干友民,郭旭生,等.添加糖蜜对胡枝子青贮品质的影响[J].中国畜牧杂志,2008,44(1):61-64.DING W R,GAN Y M,GUO X S,et al.Effects of molasses on fermentation quality of ensiled Lespedza bicolor[J].Chinese Journal of Animal Science,2008,44(1):61-64.(in Chinese)
[15] CECILIA C,ALEJANDRO B,DANIEL G,et al.Temperate forages ensiled with molasses or fresh cheese whey:Effects on conservation quality,effluent losses and ruminal degradation[J].Animal Feed Science and Technology,2011,171(1):14-19.
[16] 原现军,王奇,李志华,等.添加糖蜜对青稞秸秆和多年生黑麦草混合青贮发酵品质及营养价值的影响[J].草业学报,2013,22(3):116-123.YUAN X J,WANG Q,LI Z H,et al.Effect of molasses addition on fermentation and nutritive quality of mixed silage of hulless barley straw and perennial ryegrass in Tibet[J].Acta Prataculturae Sinica,2013,3:161-123.(in Chinese)
[17] BAYTOK E,AKSU T,KARSLI M,et al.The effects of formic acid,molasses and inoculant as silage additives on corn silage composition and ruminal fermentation characteristics in sheep[J].Turkish Journal of Veterinary Animal Sciences,2005,29(2):469-474.
[18] XIA C,LIANG Y,BAI S,et al.Effects of harvest time and added molasses on nutritional content, ensiling characteristics and in vitro degradation of whole crop wheat[J].Asian-Australasian Journal of Animal Sciences,2018,31(3):354-362.
[19] 赵亮亮,董宽虎,张瑞忠.添加不同水平糖蜜对燕麦青贮的影响[J].草原与草坪,2007,5:49-53.ZHAO L L,DONG K H,ZHANG R Z.Effects of adding different levels of molasses on the quality of oat silage[J].Grassland and Turf,2007,5:49-53.(in Chinese)
[20] ARBABI S,GHOORCHI T.The effect of different levels of molasses as silage additives on fermentation quality of foxtail millet (Setaria italica) silage[J].Asian Journal of Animal Sciences,2008,2:43-50.
[21] 杨桂英.添加糖蜜对燕麦青贮品质的影响[J].山西农业大学学报(自然科学版),2009,3:211-214.YANG G Y.Effects of adding cane molasses on the quality of oat silage[J].Journal of Shanxi Agricultural University(Natural Science Edition),2009,3:211-214.(in Chinese)
[22] NING T,LENG C,CHEN L,et al.Metabolomics analysis of serum in a rat heroin self-administration model undergoing reinforcement based on 1 H-nuclear magnetic resonance spectra[J].BMC Neuroscience,2018,19(1):4.
[23] 张文举,晏向华,龚月生,等.青贮对玉米秸营养价值及其瘤胃有效降解率的影响[J].中国草食动物,2003,1:8-9.ZHANG W J,YAN X H,GONG Y S,et al.Studies on the nutritive value and effective degradability of ensiled corn stover[J].Chinese Herbivores,2003,1:8-9.(in Chinese)
[24] 王海林,陈凤林,马慧.乳酸菌制剂对去穗玉米秸青贮营养成分及其奶牛瘤胃降解率的影响[J].中国乳业, 2013,5:26-29WANG H L,CHEN F L,MA H.Effects of lactic acid bacteria preparation on composition of corn stalk and the rumen degradability rate of dairy cows[J].China Dairy,2013,5:26-29.(in Chinese)
[25] 苗树君,马慧.不同生物制剂处理去穗玉米秸青贮对奶牛营养价值的影响[J].中国牛业科学,2008,5:27-30.MIAO S J,MA H.Effects of different biologic agents on the nutritional value of corn stalk silage[J].Chinese Cattle Science,2008,5:27-30.(in Chinese)
[26] LI M,ZI X,ZHOU H,et al.Effects of sucrose, glucose, molasses and cellulase on fermentation quality and in vitro gas production of king grass silage[J].Animal Feed Science and Technology,2014,197:206-212.
[27] 侯玉洁,徐俊,吴春华,等.5种不同牧草在奶牛瘤胃中降解特性的研究[J].中国奶牛,2013,16:4-8.HOU Y J,XU J,WU C H,et al.Study on the degradation characteristics of 5 different herbage in the rumen of dairy cows[J].China Dairy Cattle,2013,16:4-8.(in Chinese)
[28] 刘辉.优质紫花苜蓿青贮调制技术及其品质评定研究[D].兰州:甘肃农业大学,2015.LIU H.Research on quality evaluation of alfalfa silage and quality of alfalfa[D].Lanzhou:Gansu Agricultural University,2015.(in Chinese)
[29] CONTRERAS-GOVEA F E,MUCK R E,MERTENS D R,et al.Microbial inoculant effects on silage and in vitro ruminal fermentation, and microbial biomass estimation for alfalfa,and corn silages[J].Animal Feed Science and Technology,2011,163(1):2-10.
[30] PHESATCHA K,WANAPAT M.Improvement of nutritive value and in vitro ruminal fermentation of leucaena silage by molasses and urea supplementation[J].Asian-Australasian Journal of Animal Sciences,2016,29(8):1136-1144.
[31] ABDELFATTAH Z M S,ZHOU C S,TAN Z L,et al.In vitro ruminal gas production kinetics of four fodder trees ensiled with or without molasses and urea[J].Journal of Integrative Agriculture,2013,12(7):1234-1242.
[32] KANG S,WANAPAT M,NUNOI A.Effect of urea and molasses supplementation on quality of cassava top silage[J].Journal of Animal and Feed Sciences,2018,27:74-80.
[33] 辛杭书,刘凯玉,张永根,等.不同处理水稻秸秆对体外瘤胃发酵模式、甲烷产量和微生物区系的影响[J].动物营养学报,2015,27(5):1632-1640.XIN H S,LIU K Y, ZHANG Y G,et al.Effects of different treatment of rice straw on rumen fermentation mode, methane production and microflora in vitro ruminal fermentation[J].Chinese Journal of Animal Nutrition,2015,27(5):1632-1640.(in Chinese)
[34] 范华,裴彩霞,董宽虎.不同保存方法对秸秆营养价值的影响[J].中国农学通报,2007,23(11):24-28.FAN H,PEI C X,DONG K H.Effects of convervation methods on the nutritional value of straw[J].Chinese Agriculture Science Bulletin,2007,23(11):24-28.(in Chinese)
[35] 卢德勋.系统动物营养学导论[M].北京:中国农业出版社,2004.LU D X.Introduction to Animal Nutrition in Sys-tems[M].Beijing:China Agriculture Press,2004.(in Chinese)
[36] WANAPAT M,BOONNOP K,PROMKOT C,et al.Effects of alternative protein sources on rumen microbes and productivity of dairy cows[J].Maejo International Journal of Science and Technology,2011,5(1):271-276.
[37] BERGMAN E N.Energy contribution of volatile fatty acids from the gastrointestinal tract in various species[J].Physiological Reviews,1990,70:567-590.
[38] LEMOSQUET S,RIGOUT S,BACH A,et al.Glucose metabolism in lactating cows in response to isoenergetic infusions of propionic acid or duodenal glucose[J].Journal of Dairy Science,2004,87(6):1767-1777.
[39] LIMA R,LOURENÇO M,DÍAZ R F,et al.Effect of combined ensiling of sorghum and soybean with or without molasses and Lactobacilli on silage quality and in vitro rumen fermentation[J].Animal Feed Science and Technology,2010,155(2-4):122-131.
[40] MAHALA A G,KHALIFA I M.The effect of molasses levels on quality of sorghum (Sorghum bicolor) silage[J].Research Journal of Animal and Veterinary Sciences,2007,2:43-46.

Effects of Different Molasses Levels on in vitro Ruminal Fermentation Characteristics and Nutrient Degradation of Whole “Zhang Hybrid Millet” Silage

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FENG Peng, WU Hongda, ZHENG Haiyan, YANG Zhao, WANG Di, SHI Chen, MENG Fankun. Effect of Exogenous Biological Agents on Silage Quality and Ruminal Degradability of Corn Stalk and Potato Pulp Mixed Silage [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 529-538.
[2]	WANG Zhenyu, TAN Zhongfang, WANG Weiwei, MA Hao, QIN Guangyong, WANG Yanping, PANG Huili. Research Progress on Aerobic Deterioration of Silage Feed and Its Preventive Measures [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4207-4217.
[3]	XU Jinyi, CHEN Chao, WANG Haijin, WANG Huanhuan, NA Binbin, ZHENG Yulong. Research Progress on Silage and Utilization of Woody Feed [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2838-2852.
[4]	SU Lingling, TUOHUTI Ajide, ZHANG Guoting, PAREHATIJIANG Wufuer, XIE Lirong, WANG Qiong, ABULAITI Sulaiman, NUERNISA Molaniyazi, REN Juan, ZHANG Yuhua, XIAO Hailong, XIE Peng, XIAO Haixia. Effects of Whole Corn Silage and Supplementary Sodium Bicarbonate on Growth Performance,Digestibility,Serum Biochemical Indexes and Economic Benefits in Jiangyue Donkey [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2388-2396.
[5]	REN Lijuan, CHEN Yakun, SHAN Liyan, ZHANG Xiaofeng, XU Fenglian, WANG Youyue, WANG Siwei, WANG Kun, BU Dengpan, WANG Jianping, ZHAO Heping, ZHAO Liansheng. Analysis of Comprehensive Quality of Whole Plant Corn Silage Based on Principal Component and Grey Correlation Degree [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1211-1221.
[6]	ZHANG Xinxin, ZHANG Fujin, ZHANG Yao, GUO Zhigang, SUN Lin, LIU Guanghua, SHA Na. Analysis of Mycotoxin Contamination and Animal Health Risk Assessment in Silage Corn of China [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4451-4459.
[7]	CHEN Long, WEI Bingdong, ZHENG Lin, ZHANG Ying, YAN Xiaogang, YU Wei. Screening of Pychrotrophic Lactic Acid Bacteria from Changbai Mountain Soil [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3103-3113.
[8]	NUERHATI·Silafuer, MAIMAITITUERSUN·Abudukeremu, WUSIMAN·Yimiti. Effects of Amino Acid By-products on Fermentation Quality and Digestibility of Silage [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3183-3192.
[9]	ZHANG Shi, WU Qiong, YOU Huan, YANG Hao, HU Zongfu, WANG Sizhen, NIU Huaxin. Effects of Different Enzyme Preparations on Fermentation Quality of Whole Corn Silage [J]. , 2019, 46(4): 1045-1052.
[10]	LIANG Xin, TANG Qingfeng, GUO Yanxia, LI Mengwei, TANG Zhenhua, XIE Fang, PENG Kaiping, YANG Chengjian. Nutrient and Bacterial Diversity Changes of Natural Fermented Sugarcane Tops Silage [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3547-3554.
[11]	LIANG Qi, GAO Zhanjun, YU Zhu, XU Qingfang, CHENG Long, SONG Wenqi, GAO Pan, PAN Xiaoyan. Effects of Different Addition Amounts of Portulaca oleracea L.Silage on Lactation Performance of Dairy Goats [J]. , 2018, 45(8): 2204-2211.
[12]	TANG Zhenhua, YANG Chengjian, LI Mengwei, LIANG Xin, GUO Yanxia, LIANG Xianwei. Effect of Applying Lactobacillus plantarum and Lactobacillus buchneri on Quality and Aerobic Stability of Sugarcane Tops Silage [J]. , 2018, 45(7): 1824-1832.
[13]	MU Shenglong, YANG Ranran, ZHOU Bo, REN Fengyun, LIANG Mingzhen, DU Xuezhen, ZOU Caixia, HE Renchun. Effects of Lactobacillus plantarum and Lactobacillus buchneri on the Quality of Sugarcane Tops Silage [J]. , 2018, 45(5): 1226-1233.
[14]	LIU Yanfang, MA Jian, LI Shengli, YU Xiong. Starch Degradation Characteristics of Five Kinds of Silage in the Rumen of Dairy Cows [J]. , 2018, 45(4): 925-932.
[15]	WEI Jintao, WANG Hongwu, YANG Xuehai, FU Cong, ZHAO Na, CHEN Fang, FAN Qiwen. Ramie Silage and Effects of Different Crude Protein Level of Concentrate Supplement on Growth Performance and Serum Biochemical Parameters of Simmental Cattle [J]. , 2018, 45(12): 3463-3470.