外源生物制剂对玉米秸秆和马铃薯渣混贮品质及瘤胃降解率的影响

doi:10.16431/j.cnki.1671-7236.2022.02.014

Abstract

Abstract: 【Objective】 The purpose of the experiment was to study the effects of exogenous biological agents on the fermentation quality, nutritional value and 72 h rumen degradation rate of corn stalk and potato pulp mixed silage, screen efficient feed biological agents, and explore practical corn straw feed conversion technology.【Method】 The straw of grain and feed maize variety Zhongyuandan 32 was used as the experimental material.The corn variety Zhongyuan Dan 32 straw and Kexin 1 potato were used as experimental materials to mix silage in the ratio of 3∶1. A complete randomized experimental design was adopted. There were 15 treatments: enzyme preparation (CE₁, CE₂, CE₃ and CE₄), bacteria preparation (LAB) and enzyme bacteria compound treatment group (MCL₁, MCL₂, MCL₃, MCL₄, MCL₅, MCL₆, MCL₇, MCL₈ and MCL₉), at the same time, the control group (CK) without any enzyme bacteria preparation was set.After 60 days of silage fermentation, the silage quality index and rumen degradation rate of mixed silage were measured.【Result】 The sensory evaluation of each treatment and control of mixed silage reached excellent grade.The contents of lactic acid and acetic acid in enzyme treated groups and enzyme bacteria treated groups were significantly higher than those in bacteria treated group and control (P < 0.05).The contents of lactic acid in MCL₂ and MCL₄ groups were the highest, 2.82% and 2.77% respectively.Compared with the control, the contents of cellulose, neutral detergent fiber (NDF) and acid detergent fiber (ADF) in the mixed silage of each treatment group decreased significantly (P < 0.05), and the MCL₂ group was the lowest, 22.86%, 53.43% and 33.99%, respectively.The 72 h rumen degradation rate of cellulose in 1 g/3 kg cellulase addition groups (CE₁, MCL₂, MCL₆, MCL₇) were significantly higher than that in other groups (P < 0.05).【Conclusion】 The synergistic effects of enzymes, bacteria and bacterial enzymes promoted the fermentation of corn straw and potato residue mixed silage to varying degrees, improved the nutritional value of mixed silage, improved the degradation efficiency of nutrients in rumen of ruminants.Synthesizing the fermentation quality, nutritional composition and rumen degradation rate of corn straw, enzyme bacteria complex treatments > enzyme treatments > bacteria treatments, in which MCL₂ group (xylanase 1 g/3 kg, pectinase 1 g/3 kg, β-glucanase 1 g/3 kg, Lactobacillus 3 g/3 kg and cellulase 1 g/3 kg) were the best.Cellulase could reduce the contents of cellulose, ADF and NDF in corn straw mixed silage, and the optimum amount of cellulase was 1 g/3 kg.

Key words: corn stalk; potato pulp; biological agent; silage quality; ruminal degradability

CLC Number:

S816.5⁺3

FENG Peng, WU Hongda, ZHENG Haiyan, YANG Zhao, WANG Di, SHI Chen, MENG Fankun. Effect of Exogenous Biological Agents on Silage Quality and Ruminal Degradability of Corn Stalk and Potato Pulp Mixed Silage[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 529-538.

References

[1] LIU Q H, SHAO T, ZHANG J G, et al. Determination of aerobic deterioration of corn stalk silage caused by aerobic bacteria[J]. Animal Feed Science and Technology, 2013, 183(3-4): 124-131.
[2] BOKULICH N A, SUBRAMANIAN S, FAITH J J, et al. Quality filtering vastly improves diversity estimates from Illumina amplicon sequencing[J]. Nature Methods, 2012, 10(1): 57.
[3] LI X X. Biomass energy effective utilization of crop straws[J]. Advanced Materials Research, 2014, 1072: 136-139.
[4] OKINE A, HANDA M, AIBIBULA Y. Ensiling of potato pulp with or without bacterial inoculantsand its effect on fermentation quality, nutrient composition and nutritive value[J]. Animal Feed Science and Technology, 2005, 121: 329-343.
[5] 贾军. 低温乳酸复合菌系的筛选及其在玉米秸秆和马铃薯渣混合发酵饲料中的应用[D]. 大庆: 黑龙江八一农垦大学, 2017.
JIA J. Screening of lactic acid bacterial community atlow temperature and its application in mixed fermentation fodder with cornstalks and potatoresidues[D]. Daqing: Heilongjiang Bayi Agricultural University, 2017. (in Chinese)
[6] 申瑞瑞. 微生物发酵剂对马铃薯渣与玉米秸秆和大豆秸秆混贮效果的研究[D]. 保定: 河北农业大学, 2018.
SHEN R R. Effects of microbial fermentation agents on the mixed silage of potato pulp corn straw and soybean straw[D]. Baoding: Heibei Agricultural University, 2018. (in Chinese)
[7] 陶莲. 利用生物技术提高玉米秸秆利用率的研究[D]. 北京: 中国农业科学院, 2017.
TAO L. The study on applying biotechnology to improving the utilization of corn straw[D]. Beijing: China Academy of Agricultural Sciences, 2017. (in Chinese)
[8] YANG W Z, BEAUCHEMIN K A, RODE L M. A comparison of methods of adding fibrolytic enzymes to lactating cow diets[J]. Journal of Dairy Science, 2000, 83(11): 2512-2520.
[9] 冯鹏. 品种与栽培技术对玉米产量及青贮质量的影响[D]. 北京: 中国农业科学院, 2011.
FENG P. Effect of varieties and cultivation techniques on maize yield and silage quality[D]. Beijing: China Academy of Agricultural Sciences, 2011. (in Chinese)
[10] 许庆方, 玉柱, 韩建国, 等. 高效液相色谱法测定紫花苜蓿青贮中的有机酸[J]. 草原与草坪, 2007, 2: 63-65.
XU Q F, YU Z, HAN J G, et al. Determining organic acid in alfalfa silage by HPLC[J]. Grassland and Turf, 2007, 2: 63-65. (in Chinese)
[11] 冯鹏, 孙启忠. 不同比例玉米与沙打旺混贮营养物质及有毒有害物质分析[J]. 畜牧兽医学报, 2011, 42(9): 1264-1270.
FENG P, SUN Q Z. Nutrient composition and content of poisonous substances in corn and Astragalus adsurgens mixed silage[J]. Acta Veterinaria et Zootechnica Sinica, 2011, 42(9): 1264-1270. (in Chinese)
[12] 许庆方, 周禾, 玉柱, 等. 贮藏期和添加绿汁发酵液对袋装苜蓿青贮的影响[J]. 草地学报, 2006, 14(2): 129-134.
XU Q F, ZHOU H, YU Z, et al. The Effect of different storage time and dilution previous fermented juice on bagged alfalfa silage[J]. Acta Agrestia Sinica, 2006, 14(2): 129-134. (in Chinese)
[13] 冯鹏, 王晓娜, 王清郦, 等. 水肥耦合效应对玉米产量及青贮品质的影响[J]. 中国农业科学, 2012, 45(2): 376-384.
FENG P, WANG X N, WANG Q L, et al. Coupling effect of water and fertilizer on yield and silage[J]. Scientia Agricultura Sinica, 2012, 45(2): 376-384. (in Chinese)
[14] SANDRA K, ANTJE H, ALOIS K, et al. Genotypic and environmental variation in water soluble carbohydrate content of silage maize[J]. Field Crops Research, 2008, 106(3): 191-202.
[15] 郭望山, 孟庆翔. 杜马斯燃烧法与凯氏法测定饲料含氮量的比较研究[J]. 畜牧兽医学报, 2006, 37(5): 464-468.
GUO W S, MENG Q X. Comparison of Kjeldahl and dumas combustion methods for determination of nitrogen content in feedstuffs[J]. Acta Veterinaria et Zootechnica Sinica, 2006, 37(5): 464-468. (in Chinese)
[16] 张丽英. 饲料分析及饲料质量检测技术[M]. 北京: 中国农业大学出版社, 2003.
ZHANG L Y. Feed Analysis and Feed Quality Testing Technology[M]. Beijing: China Agricultural University Press, 2003. (in Chinese)
[17] ZHANG L X, LI Y L, DIAO Q Y. Effects of different microorganisms and their combinations on the nutritional value of corn[J]. Animal Nutrition, 2014, 1: 32-35.
[18] 刘洁, 刁其玉, 赵一广, 等. 肉用绵羊饲料养分消化率和有效能预测模型的研究[J]. 畜牧兽医学报, 2012, 43(8): 1230-1238.
LIU J, DIAO Q Y, ZHAO Y G, et al. Prediction of nutrien digestibility and energy concentrations using chemical compositions in meat sheep feeds[J]. Acta Veterinaria et Zootechnica Sinica, 2012, 43(8): 1230-1238. (in Chinese)
[19] 王红梅, 屠焰, 张乃锋, 等. 饲用酶制剂在反刍动物营养中的应用进展[J]. 草业学报, 2017, 26(3): 199-213.
WANG H M, TU Y, ZHANG N F, et al. Application of exogenous enzymes in ruminant nutrition[J]. Acta Praculuturae Sinica, 2017, 26(3): 199-213. (in Chinese)
[20] WANG F Q, XIE H, CHEN W. Biological pretreatment of corn stover with ligninolytic enzyme of high efficient enzymatic hydrolysis[J]. Bioresource Technology, 2013, 144(3): 572-577.
[21] TAYLAN A, EROL B M, AKIF K. Effects of formic acid, molasses and inoculant additives on corn silage composition, organic matter digestibility and microbial protein synthesis in sheep[J]. Small Ruminant Research, 2006, 61: 29-33.
[22] SOUZA C M, OLIVERIRA R L, VOLTOLONI T V, et al. Lambs fed cassava silage with added amarind residue: Silage quality, intake, digestibility, nitrogen balance, growth performance and carcass quality[J]. Animal Feed Technology, 2017, 235: 50-59.
[23] 任海伟, 窦俊伟, 赵拓, 等. 微生物发酵剂对玉米秸秆和莴笋叶混贮品质的影响[J]. 草业学报, 2016, 25(10): 142-152.
REN H W, DOU J W, ZHAO T, et al. Quality and lactic acid bacteria of mixed corn stalk and cabbage waste silage[J]. Acta Praculuturae Sinica, 2016, 25(10): 142-152. (in Chinese)
[24] FISUN K, LEVENT C M, LEVENT O. The Effect of bacteria enzyme mixture silage inoculant on the fermentation characteristic, cell wall con tents an daerobic stabilities of maize silage[J]. Pakistan Journal of Nutrition, 2008, 7(2): 222-226.
[25] 陶莲, 刁其玉. 青贮发酵对玉米秸秆品质及菌群构成的影响[J]. 动物营养学报, 2016, 28(1): 198-207.
TAO L, DIAO Q Y. Effects of ensiling on quality and bacteria composition of corn stalk[J]. Chinese Journal of Animal Nutrition, 2016, 28(1): 198-207. (in Chinese)
[26] TAO L, ZHOU H, ZHANG N F, et al. Effects of different source additives and wilt conditions on the pH value, aerobic stability, and carbohydrate and protein fractions of alfalfa silage[J]. Animal Science Journal, 2017, 88(1): 99-107.
[27] 吕文龙, 刁其玉, 闫贵龙. 不同添加剂对不带穗玉米秸秆青贮发酵品质的影响[J]. 中国畜牧兽医, 2010, 37(3): 22-26.
LYU W L, DIAO Q Y, YAN G L. Effects of silage additives on the fermentation quality of corn stover silages[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(3): 22-26. (in Chinese)
[28] 申瑞瑞, 李秋凤, 李运起, 等. 不同添加剂对薯渣与玉米秸秆混贮饲料发酵品质及牛瘤胃降解率的影响[J]. 草业学报, 2018, 27(11): 200-208.
SHEN R R, LI Q F, LI Y Q, et al. Effect of additives on fermentation quality and cattle rumende gradability of mixed potato pulpand corn straw silage[J]. Acta Praculuturae Sinica, 2018, 27(11): 200-208. (in Chinese)
[29] ADESOGAN A T, MA Z X, ROMERRO J J. Ruminant nutrition symposium: Improving cell wall digestion and animal performance with fibrolytic enzymes[J]. Journal of Animal Science, 2014, 92(4): 1317-1330.
[30] DURMIC Z, BLACHE D. Bioactive plants and plant products: Effects on animal function, health and welfare[J]. Animal Feed Science and Technology, 2012, 176(4): 150-162.
[31] SCHMIDT R J, KUNG L J. The effects of Lactobacillus buchneri with or without a homolactic bacterium on the fermentation and aerobic stability of corn silages made at different locations[J]. Journal of Dairy Science, 2010, 93, 1616-1624.
[32] 魏海燕, 闫琦, 王宪举, 等. 添加玉米粉、草粉及乳酸菌对马铃薯淀粉渣青贮发酵品质的影响[J]. 草业科学, 2019, 36(6): 1653-1661.
WEI H Y, YAN Q, WANG X J, et al. Effect of ground corn, grass meal and Lactobacillus on fermentation quality of tamato pulp silage[J]. Pratacultural Science, 2019, 36(6): 1653-1661. (in Chinese)
[33] GADO H M, SALEM A Z M, ODONGO N E. Influence of exogenous enzymes ensiled with orange pulp on digestion and growth performance in lambs[J]. Animal Feed Science and Technology, 2011, 165(1/2): 131-136.
[34] TITI H H, LUBBADEH W F. Effect of feeding cellulase enzyme on productive responses of pregnant and lactating ewes and goats[J]. Small Ruminant Research, 2004, 52(1/2): 137-143.
[35] 何长芳, 王建涛, 辛总秀. 两种处理小麦秸秆饲喂育肥羊效果试验[J]. 黑龙江畜牧兽医, 2010, 9: 82-83.
HE C F, WANG J T, XIN Z X. Effect sheep feeding on two treatments of wheat straw[J]. Heilongjiang Animal Science and Veterinary Medicine, 2010, 9: 82-83. (in Chinese)
[36] 刘瑞玲, 苏希孟. 复合酶制剂-秸秆分解剂的应用研究[J]. 畜牧兽医杂志, 2012, 31(1): 12-14.
LIU R L, SU X M. Application study on complex enzyme-straw decomposition agent[J]. Journal of Animal Science and Veterinary Medicine, 2012, 31(1): 12-14. (in Chinese)
[37] LI L, SUN Y, YUAN Z, et al. Effect of microalgae supplementation on the silage quality and anaerobic digestion performance of Manyflower silvergrass[J]. Bioresource Technology, 2015, 189: 334-340.

Effect of Exogenous Biological Agents on Silage Quality and Ruminal Degradability of Corn Stalk and Potato Pulp Mixed Silage

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