1,25-二羟维生素D3对肉鸡生长性能、骨骼矿化及小肠NaPi-Ⅱb和VDR mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2018.10.008

Abstract

Abstract:

The experiment was aimed to study the effects of 1,25-dihydroxycholecalciferol (1,25-(OH)₂-D₃) on growth performance,bone mineralization and duodenal phosphate transport gene expression.A total of 180 broilers (1 day old)were divided into 3 treatments with 6 replicates per treatment of 10 chickens per replicate.Three levels of 1,25-(OH)₂-D₃ were added to the basic diet:0,5 and 10 μg/kg,respectively.Broilers were slaughtered at 14 days of age,and femur,tibia,and duodenal mucosa were collected.Growth performance,bone mineralization and mRNA expressions of sodium phosphate transporter (NaPi-Ⅱb),nuclear vitamin D receptor (nVDR) and membrane vitamin D receptor (mVDR) were analyzed.The results showed that:Compared with the basic group,addition 5 μg/kg 1,25-(OH)₂-D₃ did not significantly affect the body weight gain,feed intake,feed and gain ration,and the weight,length,diameter,ash weight,ash content,calcium content,and phosphorus content of femur and tibia,and mRNA expression of NaPi-Ⅱb,nVDR and mVDR in the duodenum of broilers from 1 to 14 days of age (P > 0.05).Adding 10 μg/kg 1,25-(OH)₂-D₃ significantly reduced body weight gain,feed intake and feed conversion,the quality of femur and tibia,and the mRNA expression of NaPi-Ⅱb,nVDR,and mVDR in the duodenum of broilers (P < 0.05).In conclusion,adding 5 μg/kg 1,25-(OH)₂-D₃ to normal diets did not affect the growth,bone mineralization or the mRNA expression of NaPi-Ⅱb,nVDR and mVDR in duodenum of broilers.However,the addition of 10 μg/kg 1,25-(OH)₂-D₃ reduced the growth performance,skeletal mineralization and inhibits duodenal NaPi-Ⅱb,nVDR and mVDR gene expression at the transcriptional level.

Key words: 1,25-(OH)₂-D₃; broiler chicken; growth performance; bone mineralization; NaPi-Ⅱb; nVDR; mVDR

CLC Number:

S816.71

ZHANG Ning, YANG Xue, ZHANG Jinlong, WANG Zhixiang, HAN Jincheng, LU Hongmei, CHEN Cunnian. Effects of 1, 25-dihydroxycholecalciferol on Growth Performance, Bone Mineralization and mRNA Expression of NaPi-Ⅱb and VDR in Small Intestine of Broiler[J]. , 2018, 45(10): 2716-2723.

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Effects of 1, 25-dihydroxycholecalciferol on Growth Performance, Bone Mineralization and mRNA Expression of NaPi-Ⅱb and VDR in Small Intestine of Broiler

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