高非蛋白氮利用能力酵母菌的筛选与诱变

doi:10.16431/j.cnki.1671-7236.2018.10.009

Abstract

Abstract:

The shortage of protein feed resources has always been one of the bottlenecks restricting the healthy and stable development of Chinese feed industry.Single-cell proteins have great potential in solving the problem of shortage of food and protein in the world.After years of research,single-cell proteins have made great progress in strain selection,production techniques,and fermentation raw materials.However,the cost of single-cell protein feeds remains high,and has not been widely used in the feed industry.Increasing the utilization efficiency and growth rate of non-protein nitrogen (NPN) production strains for production of single cell proteins is the key to reducing the production cost of single cell proteins.In this test,strains with strong NPN use ability were screened out from multiple strains of yeast and subjected to UV irradiation to obtain mutant strains with high yeast protein yield.Through the study of 14 kinds of yeasts using different carbon and nitrogen sources,the most suitable carbon and nitrogen sources were determined.According to the ranking of the growth potential,yeast protein production and NPN utilization ability of 14 strains of yeast,yeast M(Saccharomyces cerevisiae strain YI59) and N2 (Saccharomyces cerevisiae isolate AA2) with better overall performance were screened out.Yeast M and N2 were used as starting yeast for UV irradiation mutagenesis.Using glucose,ammonium sulfate as carbon and nitrogen source,preliminary screening and rescreening were performed according to the plate colony size and the liquid fermentation cell protein production.Three strains of MU23,MU3 and MU5 with the largest colonies were finally obtained.After liquid fermentation,compared to the original yeast M,the protein contents of MU3 and MU5 were increased by 12.93% and 11.82%,respectively (P < 0.05),while the yeast protein production had no significant difference (P > 0.05);The yeast protein content of strain MU23 had no significant difference,and it's yeast protein production increased by 13.04%,reached to 0.26 g/L (P > 0.05).In conclusion,the use of ultraviolet irradiation mutagenesis could effectively improve the utilization ability of yeast NPN and the production of yeast protein.

Key words: yeast; screening; ultraviolet mutagenesis; non-protein nitrogen utilization; single cell protein

CLC Number:

S816.79

MA Xiafei, GUO Yanli, ZHANG Tieying. High Non-protein Nitrogen Utilization Capacity Yeast Screening and Mutagenesis[J]. , 2018, 45(10): 2724-2732.

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High Non-protein Nitrogen Utilization Capacity Yeast Screening and Mutagenesis

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