鸡源致病性奇异变形杆菌的分离鉴定与遗传进化分析

doi:10.16431/j.cnki.1671-7236.2018.09.027

Abstract

Abstract:

In order to understand the drug resistance,virulence and genetic characteristics of chicken source Proteus mirabilis,2 strains of bacteria (YLF1 and WS strains) were prepared by isolation from the Liangshan area,and they were identified by culture characteristics,microscopic characteristics and 16S rRNA sequence determination,and pathogenicity test.Then the sensitivity of the bacteria to 14 commonly used antibiotics (including β-lactams,ceftenes,aminoglycosides,tetracyclines,fulro quinolones and macrolides) was determined using the (K-B) method;The phylogenetic analysis of the 16S rRNA gene sequences of Proteus from 21 different sources published in GenBank was carried out.The results showed that the YLF1 and WS strains all had migratory and β-hemolytic growth characteristics.The microscopic examination showed gram-negative bacilli or cocci varied in length,the homology of them with Proteus mirabilis in GenBank through the 16S rRNA gene identification was above 99%,YLF1 and WS strains were identified as Proteus mirabilis;YLF1 and WS strains showed multiple drug resistance,the drug resistance rates were 78.6%(11/14) and 71.4%(10/14),respectively,the mortality rate of chicks was 80%,the YLF1 and WS strains had the highest homology with Japanese isolate from human,Xinjiang isolate from cattle and Guangdong isolate from soil (99.9%),and they were highly homologous to chicken source Proteus mirabilis from Henan,Xinjiang and India (98.9% to 99.3%),and the genetic relationship was close.These results indicated that Proteus mirabilis infection existed in the chicken populations of Liangshan area.The isolates had high virulence and strong resistance,it also suggested that the isolates might be from the environment,infected chickens or other animals,and there was a potential risk of zoonotic diseases.

Key words: chicken; Proteus mirabilis; isolation and identification; genetic evolution analysis

CLC Number:

S852.61⁺7

XU Rui, LI Yantao. Isolation, Identification and Genetic Evolution Analysis of Chicken Pathogenic Proteus mirabilis[J]. , 2018, 45(9): 2550-2558.

References

[1] 陆承平.最新动物病毒分类简介[J].中国病毒学,2005,20(6):682-688.LU C P.An introduction to the animal viruses in newreport[J].Virologica Sinica,2005,20(6):682-688.(in Chinese)
[2] 胡传伟,贾赟,简中友,等.水貂奇异变形杆菌的分离与鉴定[J].经济动物学报,2006,10(2):77-79.HU C W,JIA Y,JIAN Z Y,et al.Isolation and identification of Proteus mirabilis from mink[J].Journal of Economic Animal,2006,10(2):77-79.(in Chinese)
[3] 史同瑞,李丹,刘宇,等.貂奇异变形杆菌的分离及其生物学鉴定[J].中国预防兽医学报,2013,10:817-820.SHI T R,LI D,LIU Y,et al.Isolation and biological identification of Proteus mirabilis from mink[J].Chinese Journal of Preventive Veterinary Medicine,2013,10:817-820.(in Chinese)
[4] 王承东,李德生,汤纯香,等.大熊猫生殖道感染奇异变形杆菌一例[J].四川动物,2007,26(1):167.WANG C D,LI D S,TANG C X,et al.A case genital tract infection with Proteus mirabilis of panda[J].Sichuan Journal of Zoology,2007,26(1):167.(in Chinese)
[5] BISGAARD M,DAM A.Salpingitis in poultry Ⅱ.Prevalence,bacteriology,and possible pathogenesis in egg-laying chickens[J].Nord Veterinary Medecine,1981,33(2):81-89.
[6] 江益民,龙塔,侯玉泽,等.鸡奇异变形杆菌病病原的分离与鉴定[J].河南农业科学,1990,7:31-33.JIANG Y M,LONG T,HOU Y Z,et al.Isolation and identification of pathogen of Proteus mirabilis disease in chicken[J].Journal of Henan Agricultural Sciences,1990,7:31-33.(in Chinese)
[7] 许海峰.雏鸡奇异变形杆菌病的诊断[J].中国畜禽传染病,1997,5:45-47.XU H F.Diagnosis of Proteus mirabilis disease in chicks[J].Chinese Infectious Disease of Livestock and Poultry,1997,5:45-47.(in Chinese)
[8] 谌南辉,邓舜洲,郭松林,等.珍禽源奇异变形杆菌分离株微生物学特征研究[J].中国预防兽医学报,1999,4:241-245.CHEN N H,DENG S Z,GUO S L,et al.Microbiological characteristics of Proteus mirabilis in rare birds[J].Chinese Journal of Preventive Veterinary Medicine,1999,4:241-245.(in Chinese)
[9] 段二珍,夏平安,张凤华,等.狐狸奇异变形杆菌的分离与鉴定[J].中国兽医科学,2008,38(12):1050-1054.DUAN E Z,XIA P A,ZHANG F H,et al.Isolation and identification of Proteus mirabilis in fox[J].Chinese Veterinary Science,2008,38(12):1050-1054.(in Chinese)
[10] 高正琴,王春玲,邢进,等.鼠奇异变形杆菌的鉴别诊断[J].实验动物科学,2005,22(4):46-49.GAO Z Q,WANG C L,XING J,et al.Identification of Proteus mirabilis in laboratory mice[J].Laboratory Animal Science,2005,22(4):46-49.(in Chinese)
[11] 赵振鹏,杨振,林伟东,等.猪源奇异变形杆菌的分离鉴定及集群运动分析[J].中国畜牧兽医,2014,41(10):219-224.ZHAO Z P,YANG Z,LIN W D,et al.Isolation,identification and cluster motion analysis of Proteus mirabilis in pigs[J].China Animal Husbandry & Veterinary Medicine,2014,41(10):219-224.(in Chinese)
[12] 陈曦,王远微,王永,等.山羊腹泻粪便中奇异变形杆菌的分离鉴定及系统发育分析[J].中国畜牧兽医,2014,41(8):215-218.CHEN X,WANG Y W,WANG Y,et al.Isolation,identification and phylogenetic analysis of Proteus mirabilis from goat diarrhea feces[J].China Animal Husbandry & Veterinary Medicine,2014,41(8):215-218.(in Chinese)
[13] 赵远,杨凤梅,陈丽雄,等.人工哺育期腹泻婴猴奇异变形杆菌的分离与鉴定[J].中国比较医学杂志,2012,22(4):19-22.ZHAO Y,YANG F M,CHEN L X,et al.Isolation and identification of Proteus mirabilis from infantile monkeys during artificial feeding[J].Chinese Journal of Comparative Medicine,2012,22(4):19-22.(in Chinese)
[14] 王瑞君,熊筱娟.棘胸蛙烂皮病奇异变形杆菌的分离、鉴定及对药物敏感性研究[J].淡水渔业,2012,42(4):31-34.WANG R J,XIONG X J.Isolation,identification and drug sensitivity of Proteus mirabilis from bark rot of frog[J].Freshwater Fisheries,2012,42(4):31-34.(in Chinese)
[15] 张庆华,熊清明,肖琳琳,等.大黄鱼溃烂症的一种致病菌——奇异变形杆菌ZXS02菌株[J].水产学报,2005,29(6):824-830.ZHANG Q H,XIONG Q M,XIAO L L,et al.Proteus mirabilis ZXS02——A pathogen of large yellow croaker ulceration[J].Journal of Fisheries of China,2005,29(6):824-830.(in Chinese)
[16] 潘玉善,苑丽,刘建华,等.鸡大肠杆菌和奇异变形杆菌的分离、鉴定及药敏分析[J].江西农业学报,2009,21(12):156-159.PAN Y S,YUAN L,LIU J H,et al.Isolation,identification and drug sensitivity analysis of Escherichia coli and Proteus mirabilis in chicken[J].Acta Agriculturae Jiangxi,2009,21(12):156-159.(in Chinese)
[17] 焦振泉,刘秀梅,孟昭赫.16S rRNA序列同源性分析与细菌系统分类鉴定[J].国外医学(卫生学分册),1998,25(1):12-16.JIAO Z Q,LIU X M,MENG Z H.Homology analysis of 16S rRNA sequence and identification of bacterial system[J].Foreign Medical Sciences(Section of Hygiene),1998,25(1):12-16.(in Chinese)
[18] 彭涛,温博海,蹇锐,等.分支杆菌临床分离株的16S rRNA基因序列分析[J].中国人兽共患病杂志,2004,20(9):772-776.PENG T,WEN B H,JIAN R,et al.Sequence analysis of 16S rRNA gene in mycobacterium clinical isolates[J].Chinese Journal of Zoonoses,2004,20(9):772-776.(in Chinese)
[19] 朱明华,朱瑞良,马荣德,等.鸡奇异变形杆菌的分离鉴定和16S rRNA基因序列测定与系统进化分析[J].中国兽医学报,2011,31(6):804-808.ZHU M H,ZHU R L,MA R D,et al.Isolation and identification of Proteus mirabilis from chicken and sequencing and phylogenetic analysis of 16S rRNA gene[J].Chinese Journal of Veterinary Science,2011,31(6):804-808.(in Chinese)
[20] CLARRIDGE J E.Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases[J].Clinical Microbiology Reviews,2004,17(4):840-862.
[21] DRANCOURT M,BOLLET C,CARLIOZ A,et al.16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates[J].Journal of Clinical Microbiology,2000,38(10):3623-3630.
[22] JANDA J M,ABBOTT S L.Bacterial identification for publication:When is enough[J].Journal of Clinical Microbiology,2002,40(6):1887-1891.
[23] 禹文海,赵远,黄芬,等.PCR方法检测猕猴奇异变形杆菌[J].中国人兽共患病学报,2013,29(3):278-281.YU W H,ZHAO Y,HUANG F,et al.PCR detection of Proteus mirabilis in rhesus monkeys[J].Chinese Journal of Zoonoses,2013,29(3):278-281.(in Chinese)
[24] 曾超锋,余冬令.一起奇异变形杆菌食物中毒的实验室诊断及分析[J].国际医药卫生导报,2008,14(21):89-90.ZENG C F,YU D L.Laboratory diagnosis and analysis of a Proteus mirabilis food poisoning[J].International Medicine and Health Guidance News,2008,14(21):89-90.(in Chinese)
[25] 李少彤,孙凤琪,曾德荣,等.一起奇异变形杆菌引起食物中毒的病原学分析[J].中国卫生检验杂志,2002,12(5):620.LI S T,SUN F Q,ZENG D R,et al.Etiological analysis of a food poisoning by Proteus mirabilis[J].Chinese Journal of Health Laboratory Technology,2002,12(5):620.(in Chinese)
[26] COHEN-NAHUM K,SAIDEL-ODES L,RIESENBERG K,et al.Urinary tract infections caused by multi-drug resistant Proteus mirabilis:Risk factors and clinical outcomes[J].Infection,2010,38(1):41-46.

Isolation, Identification and Genetic Evolution Analysis of Chicken Pathogenic Proteus mirabilis

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