防治奶牛乳房炎中药组方的筛选及其抑菌杀菌作用研究

doi:10.16431/j.cnki.1671-7236.2018.07.033

Abstract

Abstract:

In order to provide new methods and approaches to the treatments of bovine mastitis,this study screened out the best prescription for inhibiting the main pathogens causing mastitis in dairy cows according to the principles of dialectical methods that Chinese veterinarians treat diseases.The Dandelion,Purslane,Honeysuckle,Purple Flower,Radix Paeoniae Rubra,Radix Paeoniae Alba,Chrysanthemi Flos,Scutellariae Radix,Reed and Carthami Flos were used as materials in this experiment.A preliminary screening of antibacterial Chinese herbal medicines was conducted through L₁₂ (2¹¹) orthogonal test,the drug group was further screened by the orthogonal test of L₁₆ (4⁵),and the anti-bacterial effect of Chinese medicine group on the main pathogenic bacteria of bovine mastitis was determined by Oxford cup method in vitro.The minimum antimicrobial concentration (MIC) and minimum bactericidal concentration (MBC) were detected by two-fold dilution method and plate counting.The results showed that the optimal factors of group were Paeoniae Radix Rubra, Scutellariae Radix,Carthami Flos,Paeoniae Radix Alba and Chrysanthemi Flos,and the proportion was 3:3:3:1:2.The bacterial inhibition diameters for Escherichia coli was 17.39 mm,and MIC and MBC were 62.50 and 125.00 mg/mL respectively.The bacterial inhibition diameters for Staphylococcus aureus was 25.44 mm, and MIC and MBC both were 31.25 mg/mL.In conclusion,the study successfully screened out a new traditional Chinese medicine that had good antibacterial and antiseptic effects for the bacteria causing bovine mastitis.

Key words: bovine mastitis; Chinese medicines formula; orthogonal test; MIC; MBC; Oxford cup method

CLC Number:

S859.3

SUN Jing, YE Dongyang, WANG Xurong, HOU Yanhua, WANG Lei, ZHANG Jingyan, ZHANG Kai, ZHANG Kang, LI Jianxi, YANG Zhiqiang. Study on Screening of Traditional Chinese Medicine Formula and Its Anti-bacterial Effects Preventing Bovine Mastitis[J]. , 2018, 45(7): 1990-2000.

References

[1] PELLEGRINO M,BERARDO N,GIRAUDO J,et al.Bovine mastitis prevention:Humoral and cellular response of dairy cows inoculated with lactic acid bacteria at the dry-off period[J].Beneficial Microbesb,2017,8(4):589-596.
[2] MEKONNEN S A,KOOP G,LAM T J G M,et al.The intention of North-Western Ethiopian dairy farmers to control mastitis[J].PLoS One,2017,12(8):e0182727.
[3] 林杰,王旭荣,杨志强,等.青海省某奶牛场顽固性乳房炎主要病原菌的分离鉴定与耐药性研究[J].动物医学进展,2015,37(12):98-102. LIN J,WANG X R,YANG Z Q,et al.Isolation,identification and drug-resistance of main pathogens from milk of dairy cows with intractable mastitis in Qinghai province[J].Progress in Veterinary Medicine,2015,37(12):98-102.(in Chinese)
[4] 王海瑞,王旭荣,张景艳,等.甘肃省某奶牛场肠杆菌性乳房炎主要病原菌的分离鉴定与耐药性分析[J].动物医学进展,2016,37(5):63-68. WANG H R,WANG X R,ZHANG J Y,et al.Isolation,identification and drug resistance analysis of main pathogens from a farm of dairy cows with mastitis in Gansu province[J].Progress in Veterinary Medicine,2016,37(5):63-68.(in Chinese)
[5] WU F,LI H,JIN L,et al.Deer antler base as a traditional Chinese medicine:A review of its traditional uses,chemistry and pharmacology[J].Journal Ethnopharmacology,2013,145(2):403-415.
[6] 李会芳.乳房炎康复液的药效学研究[D].杨陵:西北农林科技大学,2014. LI H F.Study on the pharmacodynamics of the Rufangyan rehabilitation solution[D].Yangling:Northwest A & F University,2014.(in Chinese)
[7] 宫尚群,范银萍,李璐琪,等.乳房按摩结合中药治疗乳痈的Meta分析[J].护理学报,2017,24(1):9-13. GONG S Q,FAN Y P,LI L Q,et al.Breast massage combined with traditional Chinese medicine for acute mastitis:A meta-analysis[J].Journal of Nursing,2017,24(1):9-13.(in Chinese)
[8] ASHOUR M L,YOUSSEF F S,GAD H A,et al.Inhibition of cytochrome P450(CYP3A4) activity by extracts from 57 plants used in traditional Chinese medicine (TCM)[J].Pharmacognosy Magazine,2017,13(50):300-308.
[9] 杨健,严作廷,王东升,等.中药治疗奶牛乳房炎的系统评价与Meta分析[J].南方农业学报,2016,47(4):656-663. YANG J,YAN Z T,WANG D S,et al.The system evaluation and analysis of the Meta for treat cow mastitis with traditional Chinese medicine[J].Journal of Southern Agriculture,2016,47(4):656-663.(in Chinese)
[10] 李林育,秦鹏,冶冬阳,等.英黄散水煎液对奶牛乳房炎致病菌的体外抑菌及抗炎效果的观察[J].中国兽医科学,2017,9:1193-1199. LI L Y,QIN P,YE D Y,et al.Bacteriostasis and anti-inflammatory in vitro of main pathogens from a farm of dairy cows with mastitis by Dandelion and Scutellaria Baicalensis Georgi[J].Chinese Veterinary Science,2017,9:1193-1199.(in Chinese)
[11] 张敬礼,王双山,袁逢新.自拟中草药方剂治疗奶牛隐性乳房炎的试验[J].中国畜牧兽医,2007,34(6):67-68. ZHANG J L,WANG S S,YUAN F X.Self-made Chinese herbal prescription for the treatment of dairycow recessive mastitis[J].China Animal Husbandry & Veterinary Medicine,2007,34(6):67-68.(in Chinese)
[12] 张代玉,周庆民,徐馨,等.中药对奶牛乳房炎主要病原菌体外抑菌试验效果的研究[J].黑龙江畜牧兽医,2016,5:206-208. ZHANG D Y,ZHOU Q M,XU X,et al.Effects of traditional Chinese medicine bacteriostasis in vitro on main pathogens of bovine mastitis[J].Heilongjiang Animal Science and Veterinary Medicine, 2016,5:206-208.(in Chinese)
[13] 刘彦晶,张雅洁,纪雪,等.中药黄芩对NDM-1乙酸钙不动杆菌的抑菌及质粒消除的研究[J].中国药学杂志,2017,52(12):1018-1022. LIU Y J,ZHANG Y J,JI X,et al.Antibacterial and NDM-1 plasmid elimination effects of Radix Scutellariae baicalensis on Acinetobacter calicoaceticus[J].Chinese Pharmaceutical Journal,2017,52(12):1018-1022.(in Chinese)
[14] WANG W,JIANG H,ZHIWEI W U,et al.Study on the bacteriostatic effects of 7 kinds of Chinese herbal medicines such as Ophiopogon japonicus and comb[J].Agricultural Science & Technology,2016,17(11):2560-2563.
[15] KOBAYASHI I,MURAOKA H,SAIKA T,et al.Micro-broth dilution method with air-dried microplate for determining MICs of clarithromycin and amoxycillin for Helicobacter pylori isolates[J].Journal of Medical Microbiology,2004,53(5):403-406.
[16] 张胜燕,钟莲.金蒲消痈汤治疗黑白花奶牛临床型乳房炎的效果[J].中国兽医杂志,2016,52(2):50-51. ZHANG S Y,ZHONG L.The therapeutic effect for clinical mastitis by consumer soup with Honeysuckle and Dandelion[J].Chinese Journal of Veterinary Medicine,2016,52(2):50-51.(in Chinese)
[17] 王礼伟,施研进,屈勇刚,等.新疆石河子地区奶牛乳房炎大肠杆菌的分离鉴定及耐药性分析[J].中国畜牧兽医,2014,41(1):203-206. WANG L W, SHI Y J,QU Y G,et al.Isolation and identification of Escherichia coli from dairy cow mastitis in Shihezi region of Xinjiang[J].China Animal Husbandry & Veterinary Medicine,2014,41(1):203-206.(in Chinese)
[18] SULEIMAN T S,KARIMURIBO E D,MDEGELA R H.Prevalence of bovine subclinical mastitis and antibiotic susceptibility patterns of major mastitis pathogens isolated in Unguja island of Zanzibar, Tanzania[J].Tropical Animal Health and Production,2017,10:1-8.
[19] 格松.主要致病菌引发的奶牛乳房炎的防治[J].甘肃畜牧兽医,2016,46(7):66-67. GE S.The prevention and treatment of cow mastitis by the major pathogenic bacteria[J].Gansu Animal Husbandry and Veterinary,2016,46(7):66-67.(in Chinese)
[20] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015. CHINESE PHARMACOPOEIA COMMISSION.Pharmacopoeia of the People's Republic of China[M].Beijing:China Medical Science Press,2015.(in Chinese)
[21] 王勇,李春,仇琪,等.中药复方多成分多靶点协同增效药理药效评价体系[J].中国科学(生命科学),2016,46(8):1029-1032. WANG Y,LI C,QIU Q,et al.Study of collaborative evaluation system on synergistic pharmacological effect of Chinese formula with multi-components andmulti-targets[J].Scientia Sinica(Vitae),2016, 46(8):1029-1032.(in Chinese)
[22] 王国庆.防治奶牛隐性乳房炎中药"乳宁散"质控、毒理及药效学研究[D].北京:中国农业科学院,2013. WANG G Q.Study on the quality control, toxicology and pharmacodynamics of "Runingsan" for treatmentor prevention of subclinical mastitis in dairy cows[D].Beijing:Chinese Academy of Agricultural Sciences,2013.(in Chinese).
[23] 司红彬,宋剑武,黄凯,等.一种含有赤芍和氟苯尼考的畜禽用复方药物[P].2014. SI H B,SONG J W,HUANG K,et al.A compound medicine with Radix Paeoniae Rubra and florfenicol for animal[P].2014.(in Chinese)
[24] 荣霞,王自力,张翥,等.抑制奶牛乳腺炎致病菌的中药复方筛选及其抗炎效果研究[J].中国兽药杂志,2015,49(9):25-31. RONG X,WANG Z L,ZHANG Z,et al.Screening of Chinese medicine formula and anti-inflammatory effects of Chinese medicine formula with antibacterial activity to mastitis pathogens[J].Chinese Journal of Veterinary Drug,2015,49(9):25-31.(in Chinese)
[25] 张鹏鹏,李增顺,汤鹏,等.赤芍水提物对金黄色葡萄球菌肠毒素及蛋白A表达的影响[J].动物医学进展,2016,37(9):75-78. ZHANG P P,LI Z S,TANG P, et al.Influence of Radix Paeoniae Rubra water extract on expression of enterotoxins and protein A of Staphylococcus aureus[J].Progress in Veterinary Medicine,2016,37(9):75-78.(in Chinese)

Study on Screening of Traditional Chinese Medicine Formula and Its Anti-bacterial Effects Preventing Bovine Mastitis

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Yanghui, XU Tao, ZHANG Shuxian, LIU Ke, JIA Tianqing, CHEN Ning, YIN Junliang, XI Linqiao, WANG Dong, ZHOU Xiaoling. Effect of Replacing Wheat Straw with Cumin Straw on Growth Performance, Slaughter Performance and Plasma Biochemical Parameters of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2296-2304.
[2]	XU Ziyan, JIANG Yang, LIU Xuetao, LIU Bohan, ZHANG Wen, YAN Changguo, CUI Lianhua. Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2312-2320.
[3]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[4]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[5]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[6]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[7]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[8]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[9]	CHEN Xi, LI Xinyi, CHEN Fashun, DUAN Yong, DONG Ling, WU Shujie, FAN Jiancheng, XIANG Bin, YANG Liangyu, ZHANG Limei. Study on the Probiotic Properties and Antimicrobial Activity of 5 Strains of Bacillus amyloliquefaciens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2082-2091.
[10]	XU Chenghui, ZHANG Xumei, YANG Tong, LI Jiahui, SUN Yawei, SHI Huijun, FU Qiang, YANG Li. Molecular Mechanism of Leonurine in the Treatment of Intestinal Inflammation Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2103-2113.
[11]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[12]	TANG Yu, YANG Yifeng, ZHANG Ying, XUE Hailong, FENG Huailiang, XU Baozeng. Cytological Study on Oocyte Maturation of Raccoon Dog [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1434-1443.
[13]	WANG Leping, PAN Yan, TAN Yizhou, LI Lei, HAN Kaiou, CAO Yajie, LI Jun, ZHOU Yuqing, LIAO Yuying, MA Dongxin, GONG Yu, PENG Hao, WANG Xiaoye. Isolation,Identification and Genome Analysis of Escherichia coli Phage vB_EcoP_GN07 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1522-1531.
[14]	HUO Mingkai, GUAN Feihu, QIU Runhui, WEI Chunyan, YU Haitao, ZHU Jiale, ZHANG Hui. Study on the Mechanism of DDX1 Inhibiting Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1556-1566.
[15]	LIU Jiaxing, HAN Xueying, ZHANG Xinru, DENG Jiaxin, YAO Lunguang, LIU Yangkun. Preparation and Immunogenicity Analysis of Ferritin Nanoparticles Carrying Antigenic Epitopes of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1567-1574.