利用DNA条形码对海南特种野猪的鉴定研究

doi:10.16431/j.cnki.1671-7236.2018.05.020

Abstract

Abstract:

This study was aimed to explore the feasibility and effectiveness of using a specific segment of pig cytochrome C oxidase subunit Ⅰ (COXⅠ) gene as DNA barcode to identify Hainan special wild boar and other species.COXⅠgene was detected in Hainan special wild boar,Hainan wild boar,Tunchang pig,Wuzhishan pig and Landrace pig,the COXⅠ gene sequences of 13 representative native pig breeds were downloaded from NCBI,the genetic diversity and genetic distance were analyzed between Hainan special wild boar and other species,and phylogenetic tree was constructed.The results showed that the sequence of pig COXⅠgene was 1 419 bp,and 67 variation sites of COXⅠ gene were detected in 45 individuals of 5 pig breeds,accounting for 4.7% of the analysis sites,and which 22 sites were parsimony information sites.The intraspecies genetic distance of 5 pig breeds was 0.001 to 0.019,the highest intraspecific genetic diversity was 0.019 of Hainan special wild boar.The interspecific genetic distance of 5 pig breeds was 0.009 to 0.123,the genetic distance between Hainan special wild boar and Landrace pig was the largest(0.123).There was obvious hybrid in Hainan special wild boar,which had clustered with Hainan native breed (Wuzhishan pigs,Tunchang pigs and Hainan wild boar).Hainan special wild boar was located in a branch with Wuyi Black pigs,Sandu Black pigs,Luchuan pig,the other part of the cluster was located in a branch with Dahuabai pig,and there was no cluster with Landrace pig.The results would provide references for the further breeding of Hainan special wild boar.

Key words: Hainan special wild boar; DNA barcode; identification

CLC Number:

S828.9⁺9

CAI Keqi, GUAN Qingfeng, PENG Wenchuan, WU Kebang. Study on Identification of Hainan Special Wild Boar by DNA Barcoding[J]. , 2018, 45(5): 1283-1288.

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Study on Identification of Hainan Special Wild Boar by DNA Barcoding

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