猪繁殖与呼吸综合征病毒(PRRSV)协同感染细菌性疾病的分析研究

doi:10.16431/j.cnki.1671-7236.2018.03.028

Abstract

Abstract:

To investigate the genetic variation and clinical infections of porcine reproductive and respiratory syndrome virus (PRRSV) pandemic strain in Hubei province,we collected 10 clinical samples (lung,lymph nodes) which were suspected infected with PRRSV,samples were detected using RT-PCR amplification of PRRSV Nsp2 gene.Futhermore,the nucleotide sequence of ORF5 gene from two PRRSV positive samples were determined,and homology comparison analysis based on Nsp2 were performed.Multiple PCR were used to detect particular pathogenic from 10 copies of pig lung disease and 12 copies of the associated nasal swab samples.The results showed that five copies in 10 clinical samples were identified as American type variable PRRSV,positive rate was 50%.ORF5 gene sequence analysis showed that all the nucleotide homology between two pandemic strain was 99.7%,and showed a highly nucleotide homology with TJM-F92,JXA1-R,HuN4-F112 about 96.7% to 97.0%;The homology rate with American standard strain VR2332 was 87.6% and 87.9%,respectively;The homology rate with classical strains of the separation of the earlier domestic (CH-1R and R98 strains) was 92.9% and 87.4%,87.7%,respectively.The main clinical common infection pattern were PRRSV+PM+SS,PRRSV+PM or PRRSV+HPS.Bacteria drug sensitive test results showed that the multiple kill pasteurella was highly sensitive against ceftriaxone,amoxicillin,etc,while Streptococcus suis was highly sensitive against amoxicillin,ampicillin,azithromycin,etc.This study revealed the clinical PRRSV strains in particular farms in Hubei province.The genetic relationship between clinical PRRSV strains and vaccine strains were also revealed.These results would provide theoretical basis for the development of new vaccines and drugs.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); bacterial disease; co-infection; prevention and control

CLC Number:

S852.65⁺9.6

LI Mingbo, KU Xugang, ZHAO Haizhong, SONG Zhongxu, SUN Hua, MEI Shuqi. Study on Co-infection Analysis of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Bacterial[J]. , 2018, 45(3): 781-789.

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Study on Co-infection Analysis of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and Bacterial

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