内皮素3对小鼠黑色素沉着相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2018.03.003

Abstract

Abstract:

The study was conducted to explore whether endothelin 3 (EDN3) could expressed in mouse skin of different hair follicle period and its influence on the expression of melanin-related genes.The expression of EDN3 and EDNRB in mouse skin of different hair follicle period were analyzed by RT-PCR technology.The results showed that EDN3 and EDNRB were both expressed in mouse skin samples in different hair follicle period,and the relative expression of EDN3 mRNA in anagen and catagen were 2.18 times (P < 0.05) and 1.15 times (P > 0.05) as much as telogen,respectively,while the EDNRB mRNA in anagen and catagen were 16.8 times (P < 0.01) and 9.9 times (P < 0.01),respectively.In order to further reveal the important role of EDN3 in pigmentation,the EDN3 were overexpressed in mice melanocytes by cell transfection technique,the melanin content of melanocytes and the relative expression levels of pigmentation-related genes were tested.The results showed that the melanin content was obviously increased after transfecting EDN3 into mice melanocytes in vitro.Furthermore,MITF mRNA was decreased (P < 0.05),TYR,TYRP2,EDNRB,c-Kit and EDN1 mRNA were significantly increased 2.04 times (P < 0.05),1.44 times (P < 0.05),1.41 times (P < 0.05),5.21 times (P < 0.01) and 3.27 times (P < 0.01).The expression of MITF protein was significantly decreased (P < 0.05);TYR,TYRP2,c-Kit and EDNRB proteins were significantly increased to 1.48 times (P < 0.01),4.61 times (P < 0.01),1.27 times (P < 0.05) and 2.64 times (P < 0.01), respectively.In conclusion,EDN3 was effectively expressed in mouse skin of different hair follicle period,and its expression levels had significant differences,and overexpressing EDN3 could increase the expression level of melanin-related genes and melanin content.

Key words: endothelin 3(EDN3); coat color; melanocyte; melanin synthesis

CLC Number:

Q786

LIU Ying, XUE Linli, CHANG Lucheng, BAI Yun, LI Lixin, LI Jingwei, YU Xiuju, HE Xiaoyan, WANG Haidong. Effects of EDN3 on the Expression of Melanin-related Genes in Mouse Melanocytes[J]. , 2018, 45(3): 581-589.

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Effects of EDN3 on the Expression of Melanin-related Genes in Mouse Melanocytes

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