酪氨酸相关蛋白酶1基因编码区系统发育分析和正选择位点检测

doi:10.16431/j.cnki.1671-7236.2015.09.032

Abstract

Abstract: Tyrosinase-related protein 1 (TYRP1) is one of the tyrosinase family members, which was also the first successfully cloned pigment gene.The study aimed at exploring the selective pressure on TYRP1 gene in the process of biological evolution.The study used NCBI to download nucleotide and protein sequences of TYRP1 gene coding regions in different species, through the Clustal X to compare TYRP1 gene encoding protein sequences, using Pal2nal online tool to generate codon multiple sequence alignment, DAMBE software was used to determine the base substitution saturation for constructing phylogenetic trees, using maximum likelihood method and PAML software to analyse TYRP1 gene in different species phylogenetic relationships and selection pressure.The results showed as follows:TYRP1 gene evolution and evolutionary classification of mammals was most close, and the site specific model revealed that TYRP1 genes were mainly affected by purifying selection, and it had been subjected to positive selection in its adaptive evolution, the software found thirteen positive selection amino acid sites.The study analyzed the evolutionary pattern of the TYRP1 gene from the molecular evolutionary perspective, providing a new way for the study of the TYRP1 gene structure and function.

Key words: TYRP1 gene; coat color; base substitution; genetic relationship; positive selection sites

CLC Number:

S813.3

LI Li-sha, LI Xiang-long, ZHOU Rong-yan, LI Lan-hui, ZHANG Yong-gai, MA Meng-yun. Phylogenetic Analysis and Detection of Positively Selected Site on the Coding Regions of TYRP1 Gene[J]. , 2015, 42(9): 2427-2435.

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Phylogenetic Analysis and Detection of Positively Selected Site on the Coding Regions of TYRP1 Gene

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