小鼠Oct-1基因CDS区克隆与生物信息学分析

doi:10.16431/j.cnki.1671-7236.2016.08.005

Abstract

Abstract:

In order to explore the biological characteristics of Oct-1 gene in mouse,CDS region of Oct-1 gene in mouse was cloned.And the sequence,physicochemical property,subcellular localization,target genes and conservative structure domain of Oct-1 gene were analyzed by bioinformatics method to predict whether the Oct-1 gene could regulate the production of melanin.The results showed that the length of Oct-1 gene in mouse was 2 313 bp.The formula of Oct-1 protein was C₃₄₂₅H₅₆₀₆N₉₇₆O₁₁₆₃S₁₅,which was an unstable and soluble protein.The secondary structure of Oct-1 mainly contained random coil and α-helices.Subcellular localization of Oct-1 was mainly in the nucleus,which Oct-1 might play a role of signal transducer or transcription regulation in the energy metabolism and cofactor biosynthesis.The Oct-1 gene regulated the expression of 13 genes associated with the melanin formation of mouse.The protein was encoded by Oct-1 gene including a homeo-domain and a POU domain.There were binding sites of Oct-1 on the promoters of MITF,TYR,TYRP1 and TYRP2. Oct-1 might interact with Brf2,Pou2af1 and Tbp to regulate the colour gene expression.We inferred that the transcription factor Oct-1 played an important role in melanogenesis and these results provided a theoretical basis for studying the coat colour formation mechanism.

Key words: Oct-1 gene; melanocytes; gene cloning; bioinformatics

CLC Number:

Q785

YANG Yu-jing, NIE Rui-qiang, XIE Jian-shan, FAN Rui-wen, DONG Chang-sheng. Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse[J]. , 2016, 43(8): 1960-1966.

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Cloning and Bioinformatics Analysis on CDS of Oct-1 Gene in Mouse

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