驯鹿干扰素β1基因的克隆及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2017.12.025

Abstract

Abstract:

This study was aimed to clone the full-length sequence of interferon beta 1 (IFNβ1) gene from the top of reindeer antler, and analyze its molecular characteristics, to lay a foundation for its biological activity and practical application. One pair of primers was designed based on the conserved sequence of IFNβ1 gene of cattle and sheep published in GenBank. IFNβ1 gene of reindeer was amplified by PCR from genome DNA extracted from mesenchyme of reindeer antler tip, and the product was cloned and sequenced. The result showed that the full length sequence of reindeer IFNβ1 gene was 561 bp, encoded 186 amino acids and contained 3 glycosylation sites. The encoded protein contained 4 alpha helixes, 4 beta fold zones and 7 beta turn angles. Compared the amino acids of IFNβ1 gene with other mammal species, the homology was 45.1% to 92.0%. The results of sequence and phylogenetic tree analysis showed that IFNβ1 gene had species diversity, the relationship was proportional to homology. This result laid the foundation for further research on the expression, biological activity and application of reindeer IFNβ1 gene.

Key words: reindeer; IFNβ1 gene; sequence analysis; genetic evolution

CLC Number:

Q785

ZHAI Jian-cheng, WANG Qiang-hui, XIA Yan-ling, LI He-ping. Cloning and Phylogenetic Analysis of IFNβ1 Gene of Reindeer[J]. , 2017, 44(12): 3570-3577.

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Cloning and Phylogenetic Analysis of IFNβ1 Gene of Reindeer

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