猪DNM2基因克隆、序列分析与组织表达谱研究

doi:10.16431/j.cnki.1671-7236.2017.09.002

Abstract

Abstract:

This study was aimed to clone porcine dynamin-2(DNM2) gene and analyze the gene structure using bioinformatics methods, the DNM2 gene mRNA level in different tissues was also investigated. We got the DNM2 gene cDNA sequence through stitching the expressed sequence tags (EST) and partial cloned sequence of DNM2 gene using RT-PCR and RACE methods. The mRNA level of porcine DNM2 gene in different tissues were detected with Real-time quantitative PCR. The results showed that DNM2 gene included an 2 616 bp whole length open reading frame (encoding 871 amino acids). DNM2 had a relative molecular mass of 98 071.30 and an isoelectric point (pI) of 7.04,and there was no signal peptide and transmembrane domain, therefore, it did not belong to the secretory protein. The DNM2 second structure contained α-helix (361 amino acids), β-sheet (53 amino acids), random coil (335 amino acids) and extended chain (122 amino acids). The sequence multi-aligned results showed that porcine DNM2 gene shared 92.6%, 91.8%, 88.6% and 89.3% of similar nucleotide sequence with that of cattle, human, mouse and rat, respectively. The phylogenetic analysis showed that DNM2 gene was highly conserved among species. Real-time quantitative PCR results indicated that the expression level of DNM2 gene was relatively higher in spleen while that was relatively lower in breast, leg muscle, fallopian tube, ovary and uterus. This research could provide the basis for the further study of the biological function of DNM2 gene.

Key words: pig; DNM2 gene; clone; sequence analysis; tissue expression

CLC Number:

PEI Yue, ZHOU Xiao-long, YANG Song-bai, ZHAO A-yong. Cloning, Sequence Analysis and Tissue Expression of Porcine DNM2 Gene[J]. , 2017, 44(9): 2549-2557.

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Cloning, Sequence Analysis and Tissue Expression of Porcine DNM2 Gene

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