辽宁地区奶牛乳房炎源大肠杆菌毒力基因及耐药基因检测分析

doi:10.16431/j.cnki.1671-7236.2017.06.036

Abstract

Abstract:

In order to understand the virulence genes and drug resistance genes carried by E.coli strains from dairy cows with mastitis in large-scale dairy farms in Liaoning region, and also provide improved programs for control and treatment of mastitis to dairy farmers, 66 strains of E.coli isolated from milk of dairy cows with clinical mastitis in several large-scale farms in Liaoning region were examined to detect 4 virulence genes and 4 drug resistance genes using PCR methods. The results showed that none of the target genes was detected in only one strain, while at least 2 and up to 7 target genes were detected in the rest of 65 strains. The detection rates of the virulence genes stx2e, eaeA, K99 and astA were 56.1%, 47.0%, 34.8% and 31.8%, respectively. In addition, the detection rate of dual virulence gene reached 43.9%, in which the genotype with the highest detection rate was eaeA/stx2e. The detection rates of the drug resistance genes sul3, sul1, cmlA and aacA4 were 87.9%, 83.3%, 40.9% and 28.8%, respectively. And the dual resistance gene detection rate was 36.4%, in which the highest detection rate was sul1/sul3 genotype; Triple drug resistance gene detection rate was 37.9%, and cmlA/sul1/sul3 presented the highest detection rate. These results confirmed that the E.coli isolated from dairy cow mastitis in large-scale dairy farms in Liaoning region had high detecting rates of sulfonamide resistance genes and chloramphenicol resistance genes, which was directly related to the drug resistance of the E.coli. These results provided important guiding significance for the prevention and control of dairy cow mastitis in Liaoning region as well as the safety of public health.

Key words: dairy cow mastitis; E.coli; virulence genes; drug resistance genes

CLC Number:

S852.61

YAO Wei, GAO Feng, CAO Ming-hui, GU Gui-bo, ZHAO Xiao-tong, YU Xue-wu, CHEN Yao, SHEN Guan-nan, WEI Yuan-yuan. Detection and Analysis of Virulence Genes and Drug Resistance Genes in E. coli Strains Isolated from Dairy Cows with Clinical Mastitis in Liaoning Region[J]. , 2017, 44(6): 1832-1839.

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Detection and Analysis of Virulence Genes and Drug Resistance Genes in E. coli Strains Isolated from Dairy Cows with Clinical Mastitis in Liaoning Region

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