环境因子及接种量对单增李斯特菌生长的影响

doi:10.16431/j.cnki.1671-7236.2017.05.033

Abstract

Abstract:

In order to explore the growth characteristics of the Listeria monocytogenes (LM90) strain under different environmental factors and inoculum size,the D_600nm value of bacteria culture broth were measured,the growth status of LM90 under different NaCl concentration,pH value,temperature and the interactions of NaCl concentration (3% to 8%,1% for per gradient),pH (6 to 9,one for per gradient),temperature (0 to 40℃,10℃ for per gradient) had been analyzed. The results showed that the strain was in the logarithmic phase when culturing for 8 to 16 h,then entered the stationary phase when culturing for 16 to 20 h,and finally went to decline phase when culturing after 20 h. It could grow well when the NaCl concentration was 0.5% to 4%. Its optimum growth pH and temperature were 7.5 and 37℃,respectively. The interaction effect of each two factor had been analyzed by variance analysis of SPSS software,and it found that the interaction effects was extremely significantly (P <0.01).The study lay a foundation for exploring the effects of environmental factors on LM90 growth and mechanisms of resistance to environmental stresses.

Key words: Listeria monocytogenes; temperature; NaCl concentration; pH; environmental factors

CLC Number:

R378

REN Jing-jing, YANG Ming-wei, YAN Gen-qiang, JIANG Jian-jun, WANG Peng-yan. Effect of Environmental Factors and Different Inoculum Size on Growth State of Listeria monocytogenes[J]. , 2017, 44(5): 1491-1497.

References

[1] 王静,刘玉敏,李春喜,等.SD-PMA-ddPCR检测食品中单核细胞增生李斯特氏菌[J].微生物学通报,2016,10:2306-2313.
[2] 夏叶,辛永萍,李肖梁,等.单核细胞增生李斯特菌σB启动子报告质粒的构建及其在应激研究中的应用[J].中国预防兽医学报,2016,38(3):211-215.
[3] 李丹丹,徐义刚,李梦圆,等.单核细胞增生李斯特氏菌实时荧光定量PCR快速检测方法的建立[J].中国畜牧兽医,2016,43(6):1453-1457.
[4] 徐义刚,李丹丹,张柏棋,等.基于双启动引物特异性检测单核细胞增生李斯特氏菌的PCR方法[J].食品工业科技,2014,35(10):86-89.
[5] Paul M,Baranzoni G M,Albonetti S,et al. Direct,quantitative detection of Listeria monocytogenes, in fresh raw whole milk by qPCR[J].International Dairy Journal,2015,41:46-49.
[6] 潘利华,刘竹青,于荟,等.单核细胞增生李斯特菌复合抑菌剂的研究[J].食品工业科技,2012,38(8):344-362.
[7] 李浩欣,段纲,项勋,等.仔猪李斯特杆菌的分离与鉴定[J].中国畜牧兽医, 2015,42(7):1883-1889.
[8] Poimenidou S V,Chatzithoma D N,Nychas G J,et al. Adaptive response of Listeria monocytogenes to heat,salinity and low pH,after habituation on cherry tomatoes and lettuce leaves[J].PLoS One,2016,11(10):1-16.
[9] Zhang Y,Carpenter C E,Broadbent J R,et al. Habituation to organic acid anions induces resistance to acid and bile in Listeria monocytogenes[J].Meat Science,2014,96(3):1152-1157.
[10] Smet C,Noriega E,Mierlo J V,et al. Influence of the growth morphology on the behavior of Salmonella, Typhimurium and Listeria monocytogenes under osmotic stress[J].Food Research International,2015,77:515-526.
[11] O'Byrne C P,Karatzas K A. The role of sigma B (sigma B) in the stress adaptations of Listeria monocytogenes:Overlaps between stress adaptation and virulence[J].Advances in Applied Microbiology, 2008,65(65):115-140.
[12] Mujahid S,Orsi R H,Boor K J,et al. Protein level identification of the Listeria monocytogenes Sigma H,Sigma L,and Sigma C regulons[J].BMC Microbiology,2013,13(1):1-15.
[13] Saucedo-Reyes D, Carrillo-Salazar J A, Reyes-Santamaría M I,et al. Effect of pH and storage conditions on Listeria monocytogenes,growth inoculated into sapote mamey (Pouteria sapota,(Jacq) H.E. Moore & Stearn) pulp[J].Food Control,2012,28(1):110-117.
[14] O'Donoghue B,Nicaogáin K,Bennett C,et al. Inhibition of Listeria monocytogenes growth by blue light is mediated by reactive oxygen species:A role for sigma B and the blue light sensor,Lmo0799[J]. Applied&Environmental Microbiology,2016,82(13):4017-4027.
[15] 付娇娇,王旭,刘海泉,等.不同培养条件下sigB对单增李斯特菌生物被膜形成的影响[J].上海海洋大学学报,2016,25(4):634-640.
[16] 张培培,刘媛,方春,等.单增李斯特菌在冷鲜猪肉中的生长预测模型比较[J].微生物学报,2011,51(12):1625-1631.
[17] 周伟,李平兰,周康,等.温度、pH及盐对植物乳杆菌素L-1作用单核细胞增生李斯特氏菌的影响[J].食品科学,2006,27(2):121-125.
[18] 张春琳,张家国.单增李斯特菌增殖的影响因素研究[J].食品研究与开发,2011,32(7):48-53.
[19] Smith J L, Liu Y,Paoli G C. How does Listeria monocytogenes combat acid conditions[J].Canadian Journal of Microbiology,2012, 59(3):141-152.
[20] 王旭,孙晓红,潘迎捷,等.单增李斯特菌WaX12及其sigB缺失突变株在不同pH下生长动力学的比较[J].食品与生物技术学报,2016,35(5):477-484.
[21] 周小红,李学英,杨宪时,等.环境因子及接种量对单增李斯特菌生长/非生长界面的影响[J].食品工业科技,2015,7:53-59.

Effect of Environmental Factors and Different Inoculum Size on Growth State of Listeria monocytogenes

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yangyang, QIN Shizhen, TANG Defu, SHAO Yuxin, SHI Zhaoguo, WANG Zheng, LI Xing, ZHAO Dongdong. Effects of Zinc, Lactobacillus on Immune and Antioxidant Function of Squabs Infected with Salmonella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2276-2285.
[2]	WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.
[3]	LI Xiaoyu, LI Ruoyu, WANG Xiao, WANG Lili, LI Jibin, LI Shuying, XU Yongping. Research Progress on the Influence of Filamentous Phages on Host Bacterial Adaptability [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2496-2506.
[4]	FAN Yimeng, WEI Yuanyuan, WANG Huiru, GA yu, ZHANG Yannan, ZHAO Qingyu, HAO Zhihui. Potential Mechanisms of Chinese Veterinary Medicine Compound Rukang Granules in the Treatment of Dairy Cow Mastitis Based on Network Pharmacology and Molecular Docking Technology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2507-2517.
[5]	NIU Yeyu, SHEN Qi, BAI Yong, SUN Panpan, SUN Na, YIN Wei, FAN Kuohai, LI Hongquan, SUN Yaogui. Anti-inflammatory and Antioxidative Effects of Extracts of Physalis Calyx seu Fructus Based on Ulcerative Colitis Model [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2531-2539.
[6]	ZHAO Qianhui, LIU Ying, JIAO Yulan, SHI Wanyu, CHEN Fuxing. Exploring of the Mechanism of Cuscuta chinensis Flavonoids in Alleviating Reproductive Damage in Offspring Rats Exposed to Bisphenol A During Pregnancy Based on Network Pharmacology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2550-2561.
[7]	CHEN Jianshi, ZHANG Ning, PU Fangxiong, MIAO Jianjia, ZENG Nan, LI Bingxue. Prediction of Bacillus anthracis Carrying Prophages and Analysis of Drug Resistance and Virulence [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2573-2584.
[8]	ZHANG Xinyue, SUN Na, SUN Panpan, ZHANG Hua, FAN Kuohai, YIN Wei, SUN Yaogui, LI Hongquan. Mechanism of Scutellarin Against Follicular Atresia Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2585-2593.
[9]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[10]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[11]	SONG Chenglei, ZHANG Zhe, WEI Yilin, YANG Songbai, DOU Yaqing, LI Chenlei, LIU Yingke, LI Xinjian, YANG Feng, LI Xiuling, WANG Kejun, QIAO Ruimin, HAN Xuelei. Effects of Different Breeds,Seasons and Month Ages on Semen Quality of Boars [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1938-1946.
[12]	CHENG Qian, GAO Qingqing, WANG Yuhe, HUAN Changchao, GAO Song. Effects of Avian Pathogenic Escherichia coli Outer Membrane Protein OmpA on Autophagy of DF-1 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1971-1980.
[13]	ZENG Dongdong, LIU Caixia, KOU Lijun, QIN He, JIN Ruijie, WANG Jing, REN Jingjing, MA Xun, JIANG Jianjun. Construction and Biological Characteristics of Lm4b_02324 Gene Deletion Strains of Listeria monocytogenes [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1981-1990.
[14]	ZENG Chengrong, LIU Xin, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, WEN Ming, ZHOU Bijun, CHEN Jiangfeng, JIANG Haibo. Research Progress on Quorum Sensing System of Aeromonas hydrophila and Its Inhibitor Application [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1991-2000.
[15]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.