牦牛源肠球菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2017.04.037

Abstract

Abstract:

17 feces samples of yak which were collected in Hongyuan county were measured with Gram staining method and 16S rRNA molecular identification in this study.8 suspected Enterococcus were separate from feces samples by bacteria purification and PCR amplification with 1 500 bp specific band. 6Enterococcus faecalis and 2Enterococcus faecium were identified through 16S rRNA sequencing.The homology analysis of the strains revealed that the homology between Enterococcus faecalis and reference strains sequence were 99.7% to 100%,that of Enterococcus faecium and reference sequence were 98.2% to 99.2%,indicating that the yak Enterococcus was highly conserved in the process of genetic evolution.The drug sensitive test results showed that the isolated strains were highly resistance to aminoglycoside antibiotics.Enterococcus faecium 11-1-2 strain was not only 5 multi-resistant,but also showed resistence to vancomycin.Enterococcus faecalis strains was most 3 multi-resistant.The antibiotics resistance results revealed that the resistance of yak Enterococcus was serious and should be taken seriously.

Key words: yak; Enterococcus; isolation and identification; antibiotics resistance test

CLC Number:

S855.1⁺1

XU Feng, ZHANG Huan-rong, LIANG Hua, ZHU Ming-xin, TANG Cheng. Isolation，Identification and Antibiotics Resistance Analysis of Enterococcus from Yak[J]. , 2017, 44(4): 1203-1210.

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Isolation，Identification and Antibiotics Resistance Analysis of Enterococcus from Yak

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