贵阳地区犬细小病毒VP2基因的克隆及遗传进化分析

doi:10.16431/j.cnki.1671-7236.2017.04.018

Abstract

Abstract:

This study was aimed to investigate popular genotype and phylogenesis of VP2 gene of canine parvovirus (CPV) in Guiyang area. Ten strains of viruses were isolated from Guiyang and the VP2 gene was amplified by PCR, cloned and sequenced. Results showed that the isolate virus grew could produce typical CPE in F81 cells, seven strains of CPV were CPV type 2a and others were CPV type 2c, named as GY-1 to GY-10. The nucleotide homologies of the isolated strains compared with 3 vaccine strains were 98.4% to 99.4%, with others reference strains nucleotide homologies within 97.7% to 99.9%.This research firstly reported the prevalence of CPV genotype was CPV type 2a in Guiyang area with such CPV type 2c, this was very important for monitoring the trend of CPV genetic variation and the development of vaccine.

Key words: canine parvovirus; genotype; VP2 gene; phylogenetic

CLC Number:

S852.65⁺5

CHEN Qiang, JI Xin-qin, DUAN Zhi-qiang, RUAN Yong, LEI Yun, LONG Dan-dan, HU Yan, WAN Biao. Cloning and Phylogenetic Analysis of VP2 Gene of Canine Parvovirus in Guiyang Area[J]. , 2017, 44(4): 1061-1068.

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Cloning and Phylogenetic Analysis of VP2 Gene of Canine Parvovirus in Guiyang Area

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