利用miRNA芯片筛选不同月龄香猪肝脏中差异表达的miRNA

doi:10.16431/j.cnki.1671-7236.2017.03.008

Abstract

Abstract:

To determine the roles of miRNA in the developmental stages of Xiang pig,we investigated the miRNA expression patterns in different months old of Xiang pigs.The total RNA from 3 and 7 months old of Xiang pigs were collected separately. Then,the total RNA were hybridized with the miRNA microarray and the threshold (fold-change>2, P<0.05) were used to identify the different expression miRNA. The quantitative Real-time PCR was performed to verify the microarray result. Compared to the 3 months old group,there were 11 miRNA down regulated and 9 miRNA were up regulated in the 7 months old group. The lowest down regulated miRNA was miR-27b whereas the highest up regulated one was miR-155.The result of quantitative Real-time PCR consistented with the microarray result. The result suggested that some of the dysregulated expression of miRNA might participate in the lipid metabolism in hepatic cells.

Key words: miRNA expression profile; Xiang pig; liver

CLC Number:

S828

LI Sheng, XU Ya-nan, NIU Xi, RAN Xue-qin. Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray[J]. , 2017, 44(3): 673-678.

References

[1] Bartel D P.microRNAs:Target recognition and regulatory functions[J].Cell,2009,136(2):215-233.
[2] Friedman R C,Farh K K,Burge C B,et al.Most mammalian mRNAs are conserved targets of microRNAs[J].Genome Res,2009,19(1):92-105.
[3] Sood P,Krek A,Zavolan M,et al.Cell-type-specific signatures of microRNAs on target mRNA expression[J].Proc Natl Acad Sci USA,2006,103(8):2746-2751.
[4] Landgraf P,Rusu M,Sheridan R,et al.A mammalian microRNA expression atlas based on small RNA library sequencing[J].Cell,2007,129(7):1401-1414.
[5] Krichevsky A M,King K S,Donahue C P,et al.A microRNA array reveals extensive regulation of microRNAs during brain development[J].RNA,2003,9(10):1274-1281.
[6] Miska E A,Alvarez-Saavedra E,Townsend M,et al.Microarray analysis of microRNA expression in the developing mammalian brain[J].Genome Biol,2004,5(9):R68.
[7] Zhao S,Zhang J,Hou X,et al.OLFML3 expression is decreased during prenatal muscle development and regulated by microRNA-155 in pigs[J].International Journal of Biological Sciences,2012,8(4):459-469.
[8] Sheng X,Wang L,Ni H,et al.Comparative analyses between skeletal muscle miRNAomes from Large White and Min pigs revealed micrornas associated with postnatal muscle hypertrophy[J].PLoS One,2016,11(6):e0156780.
[9] 李美航,仇杨,刘帅,等.过表达miR-103促进猪前体脂肪细胞分化[J].生物工程学报,2012,28(8):927-936.
[10] 刘畅,罗志宇,冉雪琴,等.贵州地方猪骨骼肌中3种microRNAs的表达差异[J].贵州农业科学,2014,42(3):112-116.
[11] 刘丽娟,杨飞,鲁慧文,等.miR-26b和miR-224在梅山与杜洛克猪卵泡中的差异表达分析[J].石河子大学学报(自然科学版),2014,32(2):164-168.
[12] 刘帅,宁小敏,李美航,等.miR-191通过调控C/EBPβ转录影响猪前体脂肪细胞分化[J].生物化学与生物物理进展,2013,40(2):165-176.
[13] 习欠云,周莲莲,李虹仪,等.不同品种猪肌肉组织miR-1和miR-133基因的表达分析[J].畜牧兽医学报,2012,43(6):843-848.
[14] Wang X.A PCR-based platform for microRNA expression profiling studies[J].RNA,2009,15(4):716-723.
[15] Fernandez-Hernando C,Suarez Y,Rayner K J,et al.microRNAs in lipid metabolism[J].Current Opinion in Lipidology,2011,22(2):86-92.
[16] Kim S Y,Kim A Y,Lee H W,et al.miR-27a is a negative regulator of adipocyte differentiation via suppressing PPARgamma expression[J].Biochemical and Biophysical Research Communications,2010,392(3):323-328.
[17] Qi R,Long D,Wang J,et al.microRNA-199a targets the fatty acid transport protein 1 gene and inhibits the adipogenic trans-differentiation of C2C12 myoblasts[J].Cell Physiol Biochem,2016,39(3):1087-1097.
[18] 孙金梅,路兴中,杨公社,等.不同经济类型猪种脂肪细胞的发育及其脂肪合成能力的比较研究[J].畜牧兽医学报,2000,31(2):131-136.
[19] 马义涛,李艳华,周辉云,等.阉割对金华猪肝脏miR-122和miR-378表达量和膻味性状的影响[J].农业生物技术学报,2013,21(8):957-964.
[20] 赵拴平,昝林森,唐中林,等.microRNA-155在长白猪和通城猪骨骼肌发育过程中的表达分析[J].畜牧兽医学报,2012,43(7):1170-1176.
[21] 朱师云,谢炳坤,梁如意,等.microRNA-21和TGFBI基因在猪骨骼肌生长发育中的表达分析[J].中国畜牧兽医,2014,41(11):191-197.
[22] 李艳华,马义涛,周辉云,等.microRNA-21参与调控金华猪肝脏生长发育[J].中国生物化学与分子生物学报,2013,29(8):791-795.

Determine the Different Expression miRNAs in Different Months Old Xiang Pigs by miRNA Microarray

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