腺病毒载体转染水牛不同原代体细胞的效果比较

doi:10.16431/j.cnki.1671-7236.2016.10.022

Abstract

Abstract:

Buffalo mammary epithelial cell,cumulus cell and fibroblasts were transfected by adenovirus vectors and compared their transfection efficiency.293 cells were transfected with pBHGloxdelE13cre and pDC316-eGFP by liposome,the virus was collected and titer was detected.Buffalo mammary epithelial cell,cumulus cell and fibroblasts were exposed to different multiplicity of infection (MOI) of adenovirus vectors.After 72 h,the cells were observed with inverted fluorescence microscope,and transfection efficiency was calculated.When the MOI was 25,50,100,200 and 400,the transfection efficiency of fibroblasts were 0.7%,7.0%,9.0%,12.5% and 34.0%,the transfection efficiency of cumulus cells were 42.5%,55.3%,57.4%,76.0% and 80.0%,the transfection efficiency of mammary epithelial cells were 88.7%,100%,100%,100% and 100%.The results showed that the transfection efficiency of mammary epithelial cell was the best,followed by cumulus cell,and fibroblast was poor.

Key words: buffalo; adenovirus vector; mammary epithelial cell; cumulus cell; fibroblasts

CLC Number:

S852.6

DUAN An-qin, PANG Chun-ying, ZHU Peng, LU Xing-rong, DENG Ting-xian, LIANG Xian-wei. Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells[J]. , 2016, 43(10): 2661-2665.

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Effect Comparison of Adenovirus Vector Transfer into Different Buffalo Cells

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