奶牛乳腺上皮细胞培养体系中疑似干/祖细胞的鉴定

doi:10.16431/j.cnki.1671-7236.2016.08.023

Abstract

Abstract:

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.

Key words: dairy cow; mammary stem/progenitor cell; label-retaining; lactation

CLC Number:

S813.1⁺1

HAO Yu-meng, ZHAO Feng, ZHANG Na, CHEN Dan, LI Qing-zhang. Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System[J]. , 2016, 43(8): 2095-2103.

References

[1] Deome K B,Faulkin L J,Bern H A,et al.Development of mammary tumors from hyper plastic alveolar nodules transplanted into gland-free mammary fat pads of female C3H mice[J].Cancer Research,1959,19(5):515-520.
[2] Daniel C W,Young L J T,Medina D,et al.The influence of mammogenic hormones on serially transplanted mouse mammary gland[J].Experimental Gerontology,1971,6(1):95-101.
[3] Martignani E,Cravero D,Miretti S,et al.Bovine mammary stem cells:New perspective for dairy science[J].Vet Q,2014,34(1):52-58.
[4] Li J X,Zhang Y,Ma L B,et al.Isolation and culture of bovine mammary epithelial stem cells[J].Journal of Veterinary Medical Science,2009,71(1):15-19.
[5] Capuco A V,Ellis S E,Hale S A,et al.Lactation persistency:Insights from mammary cell proliferation studies[J].Journal of Animal Science,2003,81(suppl 3):18-31.
[6] Capuco A V,Choudhary R K,Daniels K M,et al.Bovine mammary stem cells:Cell biology meets production agriculture[J].Animal,2012,6(3):382-393.
[7] Shinin V,Gayraud-Morel B,Gomès D,et al.Asymmetric division and cosegregation of template DNA strands in adult muscle satellite cells[J]. Nature Cell Biology,2006,8(7):677-682.
[8] Smith G H.Label-retaining epithelial cells in mouse mammary gland divide asymmetrically and retain their template DNA strands[J]. Development,2005,132(4):681-687.
[9] Capuco A V,Evock-Clover C M,Minuti A,et al.In vivo expansion of the mammary stem/progenitor cell population by xanthosine infusion[J].Experimental Biology and Medicine,2009,234(4):475-482.
[10] Motyl T,Bier?a J B,Koz?owski M,et al.Identification,quantification and transcriptional profile of potential stem cells in bovine mammary gland[J]. Livestock Science,2011,136(2):136-149.
[11] Zeng Y A,Nusse R.Wnt proteins are self-renewal factors for mammary stem cells and promote their long-term expansion in culture[J]. Cell Stem Cell,2010,6(6):568-577.
[12] Wang D,Cai C,Dong X,et al.Identification of multipotent mammary stem cells by protein C receptor expression[J].Nature,2014,517(7532):81-84.
[13] Nishizuka M,Kishimoto K,Kato A,et al.Disruption of the novel gene fad104 causes rapid postnatal death and attenuation of cell proliferation,adhesion,spreading and migration[J].Experimental Cell Research,2009,315(5):809-819.
[14] Fan X,Chen X,Deng W,et al.Up-regulated microRNA 143 in cancer stem cells differentiation promotes prostate cancer cells metastasis by modulating FNDC3B expression[J].BMC Cancer,2013,13(1):61.
[15] Choudhary R K.Identification and characterization of presumptive bovine mammary stem cells[D].The University of Maryland,Maryland,America,2011.
[16] Monzani P S,Bressan F F,Mesquita L G,et al.beta-casein gene expression by in vitro cultured bovine mammary epithelial cells derived from developing mammary glands[J].Genetics and Molecular Research,2011,10(2):604-614.
[17] Xu M N,Li S G,Zhao J Y,et al.The detection method of mammary gland bioreactor expression vector[J].Biotechnol Bull,2003,5:23.
[18] Ebner K E,Hoover C R,Hageman E C,et al.Cultivation and properties of bovine mammary cell cultures[J].Experimental Cell Research,1961,23(2):373-385.
[19] Sharma N,Kim J H,Sodhi S S,et al.Differentiation dynamics of mammary epithelial stem cells from Korean Holstein dairy cattle under ECM-free conditions[J].J Biomol Struct Dyn,2015,33(12):2633-2654.
[20] Capuco A V,Ellis S.Bovine mammary progenitor cells:Current concepts and future directions[J].Journal of Mammary Gland Biology and Neoplasia,2005,10(1):5-15.
[21] Zhou Y.Effects of growth hormone and insulin-like growth factor-1 on milk protein gene expression and nutrient uptake and cell proliferation in clonal bovine mammary epithelial cells[D].Virginia Polytechnic Institute and State University,America,2007.
[22] Sakamoto K,Komatsu T,Kobayashi T,et al.Growth hormone acts on the synthesis and secretion of alpha-casein in bovine mammary epithelial cells[J].J Dairy Res,2005,72(3):264-270.
[23] Fukudome K,Esmon C T.Molecular cloning and expression of murine and bovine endothelial cell protein C/activated protein C receptor (EPCR).The structural and functional conservation in human,bovine,and murine EPCR[J].J Biol Chem,1995,270(10):5571-5577.
[24] Schaffner F,Yokota N,Carneiro-Lobo T,et al.Endothelial protein C receptor function in murine and human breast cancer development[J].PLoS One,2013,8(4):e61071.
[25] Esmon C T.Structure and functions of the endothelial cell protein C receptor[J].Crit Care Med,2004,32(5):298-301.

Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	FAN Yimeng, WEI Yuanyuan, WANG Huiru, GA yu, ZHANG Yannan, ZHAO Qingyu, HAO Zhihui. Potential Mechanisms of Chinese Veterinary Medicine Compound Rukang Granules in the Treatment of Dairy Cow Mastitis Based on Network Pharmacology and Molecular Docking Technology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2507-2517.
[2]	HU Honglian, LI Chao, CHEN Liqing, SONG Liwen, ZHAO Meng, ZHANG Jianxia, WANG Dian, GAO Min. Effects of Dietary β-carotene Supplementation on Lactation Performance, Blood Routine,Serum Biochemical and Immune Indexes of Dairy Cows during the Lactating Peak Period [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1375-1384.
[3]	ZHANG Xinke, ZHU Xuedan, CHEN Yun, ZHANG Shouquan. Research Progress on Bone Mineral Density and Its Relationship with Mammalian Production [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1422-1433.
[4]	LI Dan, ZHANG Haisen, WANG Yiqun, GAO Dengke, ZHAO Hongcong, LI Chao, JIN Yaping, CHEN Huatao. Eukaryotic Expression Vector Construction,Bioinformatics and Tissue Expression Profiles Analysis of CRY1 Gene in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 870-881.
[5]	KONG Weiyi, WANG Feifei, YUE Kang, QIN Junjie, ZHU Hao, GUO Yansheng. Effect of Guiqi Yimu Oral Liquid on Plasma Metabolites in Postpartum Dairy Cows Based on UPLC-MS/MS Metabolomics Technology [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 913-923.
[6]	HE Yue, WANG Hui, CHEN Ruipeng, YU Zhixue, TANG Xiangfang, GUO Yuming, XIONG Benhai. Research Progress on Optical and Electrochemical Biosensors for the Diagnosis of Important Diseases in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 827-837.
[7]	FU Qi, YU Houmo, ZHANG Hailiang, XU Jingyi, HU Yuyang, DUAN Yujiao, ZHOU Yueling, HUANG Siqi, ZHANG Yayi, GUO Gang, YU Ying, WANG Yachun. Influencing Factors Analysis and Genetic Parameters Estimation of Muzzle Width in Holstein Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 230-237.
[8]	AN Xiaopeng, LIU Nan, HU Zhengjiamei, HU Jincao, XU Xiaolong, ZHANG Lei, SONG Yuxuan. Comparative Analysis of Lactation Performance of East Friesen Sheep, Hu Sheep and East Friesen-Hu Hybrid Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 238-245.
[9]	SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.
[10]	JI Haonan, GAO Qing, CHANG Yao, ZHANG Hailiang, WU Hongjun, CHAI He, YU Ying, WANG Yachun. Analysis of Influencing Factors on Milking Speed Indicators in Holstein Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3475-3483.
[11]	SHAO Mingzhu, ZHAO Yunqing, LI Songli, HOU Shaohua, ZHANG Yanying. Isolation and Identification of Escherichia coli from Dairy Cows in Hebei Province and Its Pathogenicity and Antibacterial Effect of Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3683-3691.
[12]	CAI Meng, ZHU Xiaoyan, WANG Mengling, LIU Zihao, XIONG Benhai, YANG Liang. Effect of Staphylococcus aureus on Exosome Characterization of Mammary Epithelial Cells in Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3091-3098.
[13]	LI Shangru, SONG Jiamei, ZHANG Chengrui, SUN Yukun, ZHANG Yonggen. Study on Dairy Cow Disease Prediction Model Based on Machine Learning Algorithm [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2534-2546.
[14]	XU Xiaoling, BAI Jiahua, XIAO Linli, QIN Yusheng, SONG Yuqing, LIU Yan. Effects of Compound Additives on the Quality and Conception Rate of Dairy Cows Thawed Sex Controlled Frozen Semen [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2654-2660.
[15]	SUN Yan, FENG Xiaowei, LIU Jiawei, LIU Bei, JIN Tianxiong, FENG Mingque, LIU Mingchao. Isolation,Identification and Antibacterial Activity of Lactic Acid Bacteria from Reproductive Tract of Healthy Dairy Cows [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1852-1859.